一种新型 C2H2- 型锌指转录因子 CitZAT4 调节乙烯诱导的萨摩蜜柑(Citrus unshiu Marc.)

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Quan Sun, Zhengchen He, Junli Ye, Ranran Wei, Di Feng, Yingzi Zhang, Lijun Chai, Yunjiang Cheng, Qiang Xu, Xiuxin Deng
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引用次数: 0

摘要

乙烯处理可促进萨摩蜜柑(Citrus unshiu Marc.)果实黄皮的橙色着色,但相应的调控机制在很大程度上仍不清楚。在这项研究中,我们发现了一种 C2H2 型锌指转录因子 CitZAT4,它的表达受到乙烯的明显诱导。CitZAT4 直接与 CitPSY 启动子结合并激活其表达,从而促进类胡萝卜素的生物合成。在萨摩蜜柑果实中的瞬时表达和柑橘胼胝体的稳定转化表明,过表达 CitZAT4 会抑制 CitLCYE 的表达,从而抑制α-支黄色类胡萝卜素(叶黄素)的生物合成。过量表达 CitZAT4 还会提高 CitLCYB、CitHYD 和 CitNCED2 的转录水平,促进类胡萝卜素的β-橙色分支积累。分子生化实验,包括酵母单杂交(Y1H)、电泳迁移(EMSA)、染色质免疫沉淀定量聚合酶链反应(ChIP-qPCR)和荧光素酶(LUC)实验,证明了CitZAT4可直接与其靶基因的启动子结合并调控其表达。研究发现,乙烯信号诱导的乙烯响应因子 CitERF061 可直接与 CitZAT4 启动子结合并诱导其表达,从而正向调节 CitZAT4 介导的柑橘果实橙色着色。综上所述,我们的研究结果表明,乙烯通过 CitERF061 驱动 CitZAT4 介导的转录级联,将乙烯信号与类胡萝卜素代谢联系起来,促进萨摩柑果实黄皮中的橙色着色。本文揭示的分子调控机制是朝着开发提高柑橘作物品质和经济效益的战略迈出的重要一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel C2H2-type zinc-finger transcription factor, CitZAT4, regulates ethylene-induced orange coloration in Satsuma mandarin flavedo (Citrus unshiu Marc.).

Ethylene treatment promotes orange coloration in the flavedo of Satsuma mandarin (Citrus unshiu Marc.) fruit, but the corresponding regulatory mechanism is still largely unknown. In this study, we identified a C2H2-type zinc-finger transcription factor, CitZAT4, the expression of which was markedly induced by ethylene. CitZAT4 directly binds to the CitPSY promoter and activates its expression, thereby promoting carotenoid biosynthesis. Transient expression in Satsuma mandarin fruit and stable transformation of citrus calli showed that overexpressing of CitZAT4 inhibited CitLCYE expression, thus inhibiting α-branch yellow carotenoid (lutein) biosynthesis. CitZAT4 overexpression also enhanced the transcript levels of CitLCYB, CitHYD, and CitNCED2, promoting β-branch orange carotenoid accumulation. Molecular biochemical assays, including yeast one-hybrid (Y1H), electrophoretic mobility shift (EMSA), chromatin immunoprecipitation quantitative polymerase chain reaction (ChIP-qPCR), and luciferase (LUC) assays, demonstrated that CitZAT4 directly binds to the promoters of its target genes and regulates their expression. An ethylene response factor, CitERF061, which is induced by ethylene signaling, was found to directly bound to the CitZAT4 promoter and induced its expression, thus positively regulating CitZAT4-mediated orange coloration in citrus fruit. Together, our findings reveal that a CitZAT4-mediated transcriptional cascade is driven by ethylene via CitERF061, linking ethylene signaling to carotenoid metabolism in promoting orange coloration in the flavedo of Satsuma mandarin fruit. The molecular regulatory mechanism revealed here represents a significant step toward developing strategies for improving the quality and economic efficiency of citrus crops.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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