利用 YBX3 基因敲除和过表达细胞系,探索 YBX3 在 PEDV 感染中的作用。

IF 1.9 4区 医学 Q3 GENETICS & HEREDITY
Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI:10.1007/s11262-024-02109-z
Jiayun Wu, Huizhen Gao, Haoyu Rui, Pan Xu, Ligang Ni, Junsheng Zhang, Ligang Wang
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引用次数: 0

摘要

猪流行性腹泻(PED)是由猪流行性腹泻病毒(PEDV)引起的一种高度传染性疾病,会造成重大经济损失。PEDV 感染会严重破坏小肠的中肠屏障。YBX3 是紧密连接中的一种重要蛋白质,可促进上皮细胞增殖。然而,它在 PEDV 感染过程中的作用仍不清楚。本研究观察到,PEDV 感染后,YBX3 的 mRNA 表达量显著增加。此外,YBX3 的蛋白表达也出现了先增加后减少的现象。此外,用 2% DSS 处理会导致 YBX3 mRNA 和蛋白表达的显著下调。此外,我们还成功生成了 YBX3 基因敲除和过表达细胞系。初步实验表明,YBX3的高表达抑制了PEDV的复制,而YBX3的敲除则产生了相反的效果。总之,我们的研究初步揭示了 YBX3 在 PEDV 感染过程中的功能作用。这一发现为今后进一步研究其机制奠定了基础,同时也为研究 PEDV 与宿主的相互作用机制提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exploring the role of YBX3 in PEDV infection through the utilization of YBX3 knockout and overexpression cell lines.

Porcine epidemic diarrhea (PED) is a highly contagious disease caused by the porcine epidemic diarrhea virus (PEDV), which results in significant economic losses. PEDV infection causes severe damage to the midgut barrier in the small intestine. YBX3, an important protein in tight junctions, promotes epithelial cell proliferation. However, its role in the process of PEDV infection remains unclear. In this study, we observed a significant increase in mRNA expression of YBX3 following PEDV infection. Additionally, the protein expression of YBX3 showed an initial increase followed by a decrease over time. Furthermore, treatment with 2% DSS resulted in a significant down-regulation of YBX3 mRNA and protein expression. Furthermore, we successfully generated knockout and overexpression cell lines of YBX3. Preliminary assays indicated that elevated expression of YBX3 inhibited the PEDV replication, while knockout of YBX3 had the opposite effect. In conclusion, our study has preliminarily revealed the functional role of YBX3 during PEDV infection. This finding lays the foundation for further investigation into its mechanism in future and also provides new insights into the mechanism of PEDV-host interactions.

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来源期刊
Virus Genes
Virus Genes 医学-病毒学
CiteScore
3.30
自引率
0.00%
发文量
76
审稿时长
3 months
期刊介绍: Viruses are convenient models for the elucidation of life processes. The study of viruses is again on the cutting edge of biological sciences: systems biology, genomics, proteomics, metagenomics, using the newest most powerful tools. Huge amounts of new details on virus interactions with the cell, other pathogens and the hosts – animal (including human), insect, fungal, plant, bacterial, and archaeal - and their role in infection and disease are forthcoming in perplexing details requiring analysis and comments. Virus Genes is dedicated to the publication of studies on the structure and function of viruses and their genes, the molecular and systems interactions with the host and all applications derived thereof, providing a forum for the analysis of data and discussion of its implications, and the development of new hypotheses.
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