Minsoo Son, Marie L Laury, Kevin B Stephenson, Thaddaeus May, D Taylor Hendrixson, Aminata Shamit Koroma, Amara Stevens Ngegbai, Jong Hee Song, Nino Naskidashvili, Young Ah Goo, Mark J Manary
{"title":"牛奶对塞拉利昂中度营养不良儿童肠道通透性、粪便 16S rRNA 基因微生物群分析和粪便代谢组学的影响：一项双盲随机对照试验。","authors":"Minsoo Son, Marie L Laury, Kevin B Stephenson, Thaddaeus May, D Taylor Hendrixson, Aminata Shamit Koroma, Amara Stevens Ngegbai, Jong Hee Song, Nino Naskidashvili, Young Ah Goo, Mark J Manary","doi":"10.1016/j.ajcnut.2024.09.018","DOIUrl":null,"url":null,"abstract":"<p><strong>Background & aims: </strong>Our objectives were to determine the effect of dietary milk protein and milk carbohydrate on the intestinal permeability, fecal 16S rRNA gene configuration, and fecal metabolomics of children with moderate malnutrition.</p><p><strong>Methods: </strong>This was a randomized, double-blind, controlled trial among 413 children with wasting in rural Sierra Leone who received one of four supplementary foods. The foods differed in sources of protein and carbohydrate: milk protein and milk carbohydrate (MPMC), milk protein and vegetable carbohydrate (MPVC), vegetable protein and milk carbohydrate (VPMC), or a control group consuming entirely vegetable-based food (VPVC). After 4 weeks, urine and stool were collected from participants enrolled with mid-upper arm circumference < 12.1 cm. Urine was analyzed for lactulose excretion (%L). Stools underwent both 16S rRNA gene analysis to assess β diversity and untargeted metabolomic abundance.</p><p><strong>Results: </strong>Among the 386 children who completed permeability testing, the mean difference (95% CI) in %L excretion as compared with VPVC was 0.01 (-0.05, 0.07) for MPMC, 0.05 (-0.01, 0.11) for MPVC, and 0.01 (-0.05, 0.07) for VPMC. Of the 374 children who provided a stool sample that was analyzed , the β diversity among bacterial taxa was similar between dietary groups (P>0.05 for all comparisons). No significant differences between dietary groups were seen among the 20 most abundant bacterial taxa. Among the 5,769 unique metabolomic features identified, greater flavonoid levels in VPVC were seen.</p><p><strong>Conclusions: </strong>Abnormal intestinal permeability did not improve with 4 weeks of supplementary feeding. Fecal rRNA did not differ with consumption of different diets. Trial registration ClinicalTrials.gov (NCT04216043).</p><p><strong>Trial registration: </strong>Clinicaltrails.gov NCT04216043URL of registration: https://clinicaltrials.gov/study/NCT04216043?id=NCT04216043&rank=1.</p>","PeriodicalId":50813,"journal":{"name":"American Journal of Clinical Nutrition","volume":null,"pages":null},"PeriodicalIF":6.5000,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The impact of milk on gut permeability, fecal 16S rRNA gene microbiota profiling and fecal metabolomics in children with moderate malnutrition in Sierra Leone: a double-blind, randomized controlled trial.\",\"authors\":\"Minsoo Son, Marie L Laury, Kevin B Stephenson, Thaddaeus May, D Taylor Hendrixson, Aminata Shamit Koroma, Amara Stevens Ngegbai, Jong Hee Song, Nino Naskidashvili, Young Ah Goo, Mark J Manary\",\"doi\":\"10.1016/j.ajcnut.2024.09.018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background & aims: </strong>Our objectives were to determine the effect of dietary milk protein and milk carbohydrate on the intestinal permeability, fecal 16S rRNA gene configuration, and fecal metabolomics of children with moderate malnutrition.</p><p><strong>Methods: </strong>This was a randomized, double-blind, controlled trial among 413 children with wasting in rural Sierra Leone who received one of four supplementary foods. The foods differed in sources of protein and carbohydrate: milk protein and milk carbohydrate (MPMC), milk protein and vegetable carbohydrate (MPVC), vegetable protein and milk carbohydrate (VPMC), or a control group consuming entirely vegetable-based food (VPVC). After 4 weeks, urine and stool were collected from participants enrolled with mid-upper arm circumference < 12.1 cm. Urine was analyzed for lactulose excretion (%L). Stools underwent both 16S rRNA gene analysis to assess β diversity and untargeted metabolomic abundance.</p><p><strong>Results: </strong>Among the 386 children who completed permeability testing, the mean difference (95% CI) in %L excretion as compared with VPVC was 0.01 (-0.05, 0.07) for MPMC, 0.05 (-0.01, 0.11) for MPVC, and 0.01 (-0.05, 0.07) for VPMC. Of the 374 children who provided a stool sample that was analyzed , the β diversity among bacterial taxa was similar between dietary groups (P>0.05 for all comparisons). No significant differences between dietary groups were seen among the 20 most abundant bacterial taxa. Among the 5,769 unique metabolomic features identified, greater flavonoid levels in VPVC were seen.</p><p><strong>Conclusions: </strong>Abnormal intestinal permeability did not improve with 4 weeks of supplementary feeding. Fecal rRNA did not differ with consumption of different diets. Trial registration ClinicalTrials.gov (NCT04216043).</p><p><strong>Trial registration: </strong>Clinicaltrails.gov NCT04216043URL of registration: https://clinicaltrials.gov/study/NCT04216043?id=NCT04216043&rank=1.</p>\",\"PeriodicalId\":50813,\"journal\":{\"name\":\"American Journal of Clinical Nutrition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.5000,\"publicationDate\":\"2024-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American Journal of Clinical Nutrition\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.ajcnut.2024.09.018\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American Journal of Clinical Nutrition","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.ajcnut.2024.09.018","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
The impact of milk on gut permeability, fecal 16S rRNA gene microbiota profiling and fecal metabolomics in children with moderate malnutrition in Sierra Leone: a double-blind, randomized controlled trial.
Background & aims: Our objectives were to determine the effect of dietary milk protein and milk carbohydrate on the intestinal permeability, fecal 16S rRNA gene configuration, and fecal metabolomics of children with moderate malnutrition.
Methods: This was a randomized, double-blind, controlled trial among 413 children with wasting in rural Sierra Leone who received one of four supplementary foods. The foods differed in sources of protein and carbohydrate: milk protein and milk carbohydrate (MPMC), milk protein and vegetable carbohydrate (MPVC), vegetable protein and milk carbohydrate (VPMC), or a control group consuming entirely vegetable-based food (VPVC). After 4 weeks, urine and stool were collected from participants enrolled with mid-upper arm circumference < 12.1 cm. Urine was analyzed for lactulose excretion (%L). Stools underwent both 16S rRNA gene analysis to assess β diversity and untargeted metabolomic abundance.
Results: Among the 386 children who completed permeability testing, the mean difference (95% CI) in %L excretion as compared with VPVC was 0.01 (-0.05, 0.07) for MPMC, 0.05 (-0.01, 0.11) for MPVC, and 0.01 (-0.05, 0.07) for VPMC. Of the 374 children who provided a stool sample that was analyzed , the β diversity among bacterial taxa was similar between dietary groups (P>0.05 for all comparisons). No significant differences between dietary groups were seen among the 20 most abundant bacterial taxa. Among the 5,769 unique metabolomic features identified, greater flavonoid levels in VPVC were seen.
Conclusions: Abnormal intestinal permeability did not improve with 4 weeks of supplementary feeding. Fecal rRNA did not differ with consumption of different diets. Trial registration ClinicalTrials.gov (NCT04216043).
Trial registration: Clinicaltrails.gov NCT04216043URL of registration: https://clinicaltrials.gov/study/NCT04216043?id=NCT04216043&rank=1.
期刊介绍：
American Journal of Clinical Nutrition is recognized as the most highly rated peer-reviewed, primary research journal in nutrition and dietetics.It focuses on publishing the latest research on various topics in nutrition, including but not limited to obesity, vitamins and minerals, nutrition and disease, and energy metabolism.
Purpose:
The purpose of AJCN is to:
Publish original research studies relevant to human and clinical nutrition.
Consider well-controlled clinical studies describing scientific mechanisms, efficacy, and safety of dietary interventions in the context of disease prevention or health benefits.
Encourage public health and epidemiologic studies relevant to human nutrition.
Promote innovative investigations of nutritional questions employing epigenetic, genomic, proteomic, and metabolomic approaches.
Include solicited editorials, book reviews, solicited or unsolicited review articles, invited controversy position papers, and letters to the Editor related to prior AJCN articles.
Peer Review Process:
All submitted material with scientific content undergoes peer review by the Editors or their designees before acceptance for publication.
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