鉴定参与宫颈癌发展的 circRNA 介导的竞争性内源性 RNA 网络。

IF 2.3 3区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

Molecular and Cellular Probes Pub Date : 2024-10-10 DOI:10.1016/j.mcp.2024.101984

Shaosheng Lou , Wang Yang , Qian Zhao , Yunshan Ouyang , Lingling Cao , Chen Lin

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引用次数: 0

摘要

背景目的：本研究旨在鉴定宫颈癌中差异表达的circRNA，并验证circ_0008193 ceRNA在宫颈癌细胞中的网络表达：方法：利用 GEO2R 工具从 GSE102686 和 GSE113696 中获取宫颈癌组织和宫颈癌细胞中差异表达的 circRNAs，以折叠变化的绝对对数值大于 1 和 p 值小于 0.05 为标准。利用相关信息学数据库预测了下游 miRNA 和 mRNA。在 Cytoscape 的帮助下，进行了 circRNA-miRNA-mRNA 相互作用网络的研究。利用 RT-qPCR、双荧光素酶报告实验和细胞实验验证了 Circ_0008193-miR-182-5p-PTEN 轴的表达水平和细胞功能：结果：GSE102686和GSE113696数据库重叠了7个差异表达的circRNA，5个circRNA具有相同的表达模式。根据文献和表达模式，建立了 circRNA-miRNA-mRNA 网络。circ_0008193、miR-182-5p和PTEN的表达模式分别为下调、上调和下调。过表达的circ_0008193抑制了宫颈癌细胞的增殖、迁移和侵袭。MiR-182-5p削弱了circ_0008193对细胞行为的抑制作用，而PTEN则抵消了miR-182-5p的作用：结论：这项研究揭示了宫颈癌中存在一个circRNA-miRNA-mRNA网络，并初步验证了circ_0008193-miR-182-5p-PTEN轴在宫颈癌细胞中的功能，为研究宫颈癌的分子机制提供了更多的指导。

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Identification of circRNA-mediated competing endogenous RNA network involved in the development of cervical cancer

Background

The abnormal expression of circRNA may contribute to the progression of cervical cancer by influencing the biological processes.

Aim

This study aimed to identify the differentially expressed circRNAs in cervical cancer and validate the circ_0008193 ceRNA network in cervical cancer cells.

Methods

Using the absolute log2 value of fold change >1 and p-value of <0.05, the differentially expressed circRNAs were obtained from GSE102686 and GSE113696 from cervical cancer tissues and cervical cancer cells with the help of the GEO2R tool. Downstream miRNAs and mRNAs were predicted using relevant informatics databases. The circRNA-miRNA-mRNA interaction network was conducted with the assistance of Cytoscape. Circ_0008193-miR-182-5p-PTEN axis was validated with expression level and cell function using RT-qPCR, a dual-luciferase reporter assay, and cellular experiments.

Results

GSE102686 and GSE113696 databases overlapped 7 differentially expressed circRNAs and five circRNAs have the same expression pattern. Based on the literature and expression pattern, a circRNA-miRNA-mRNA network was conducted. The circ_0008193, miR-182-5p, and PTEN expression patterns were downregulation, upregulation, and downregulation, respectively. Overexpressed circ_0008193 suppressed proliferation, migration, and invasion of cervical cancer cells. MiR-182-5p diminished the inhibitory influence of circ_0008193 on cellular behaviors, while PTEN counteracted the effect of miR-182-5p.

Conclusion

This investigation revealed the existence of a circRNA-miRNA-mRNA network in cervical cancer, and preliminary verified the function of circ_0008193-miR-182-5p-PTEN axis in cervical cancer cells, which offers additional guidance on investigating the molecular mechanisms of cervical cancer.

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来源期刊

Molecular and Cellular Probes 生物-生化研究方法

CiteScore

6.80

自引率

0.00%

发文量

审稿时长

16 days

期刊介绍： MCP - Advancing biology throughâ€“omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.