Zhiqin Ren, Ding Ye, Naike Su, Chaofan Wang, Lijia He, Houpeng Wang, Mudan He, Yonghua Sun
{"title":"foxl2l 是斑马鱼卵子发生的生殖细胞内在守门员。","authors":"Zhiqin Ren, Ding Ye, Naike Su, Chaofan Wang, Lijia He, Houpeng Wang, Mudan He, Yonghua Sun","doi":"10.24272/j.issn.2095-8137.2024.046","DOIUrl":null,"url":null,"abstract":"<p><p>Zebrafish serve as a valuable model organism for studying germ cell biology and reproductive processes. The AB strain of zebrafish is proposed to exhibit a polygenic sex determination system, where most males initially develop juvenile ovaries before committing to male fate. In species with chromosomal sex determination, gonadal somatic cells are recognized as key determinants of germ cell fate. Notably, the loss of germ cells in zebrafish leads to masculinization, implying that germ cells harbor an intrinsic feminization signal. However, the specific signal triggering oogenesis in zebrafish remains unclear. In the present study, we identified <i>foxl2l</i> as an oocyte progenitor-specific gene essential for initiating oogenesis in germ cells. Results showed that <i>foxl2l</i>-knockout zebrafish bypassed the juvenile ovary stage and exclusively developed into fertile males. Further analysis revealed that loss of <i>foxl2l</i> hindered the initiation of oocyte-specific meiosis and prevented entry into oogenesis, leading to premature spermatogenesis during early gonadal development. Furthermore, while mutation of the pro-male gene <i>dmrt1</i> led to fertile female differentiation, simultaneous disruption of <i>foxl2l</i> in <i>dmrt1</i> mutants completely blocked oogenesis, with a large proportion of germ cells arrested as germline stem cells, highlighting the crucial role of <i>foxl2l</i> in oogenesis. Overall, this study highlights the unique function of <i>foxl2l</i> as a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 5","pages":"1116-1130"},"PeriodicalIF":4.0000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11491788/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>foxl2l</i> is a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.\",\"authors\":\"Zhiqin Ren, Ding Ye, Naike Su, Chaofan Wang, Lijia He, Houpeng Wang, Mudan He, Yonghua Sun\",\"doi\":\"10.24272/j.issn.2095-8137.2024.046\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Zebrafish serve as a valuable model organism for studying germ cell biology and reproductive processes. The AB strain of zebrafish is proposed to exhibit a polygenic sex determination system, where most males initially develop juvenile ovaries before committing to male fate. In species with chromosomal sex determination, gonadal somatic cells are recognized as key determinants of germ cell fate. Notably, the loss of germ cells in zebrafish leads to masculinization, implying that germ cells harbor an intrinsic feminization signal. However, the specific signal triggering oogenesis in zebrafish remains unclear. In the present study, we identified <i>foxl2l</i> as an oocyte progenitor-specific gene essential for initiating oogenesis in germ cells. Results showed that <i>foxl2l</i>-knockout zebrafish bypassed the juvenile ovary stage and exclusively developed into fertile males. Further analysis revealed that loss of <i>foxl2l</i> hindered the initiation of oocyte-specific meiosis and prevented entry into oogenesis, leading to premature spermatogenesis during early gonadal development. Furthermore, while mutation of the pro-male gene <i>dmrt1</i> led to fertile female differentiation, simultaneous disruption of <i>foxl2l</i> in <i>dmrt1</i> mutants completely blocked oogenesis, with a large proportion of germ cells arrested as germline stem cells, highlighting the crucial role of <i>foxl2l</i> in oogenesis. Overall, this study highlights the unique function of <i>foxl2l</i> as a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.</p>\",\"PeriodicalId\":48636,\"journal\":{\"name\":\"Zoological Research\",\"volume\":\"45 5\",\"pages\":\"1116-1130\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11491788/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zoological Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.24272/j.issn.2095-8137.2024.046\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zoological Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.24272/j.issn.2095-8137.2024.046","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
foxl2l is a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.
Zebrafish serve as a valuable model organism for studying germ cell biology and reproductive processes. The AB strain of zebrafish is proposed to exhibit a polygenic sex determination system, where most males initially develop juvenile ovaries before committing to male fate. In species with chromosomal sex determination, gonadal somatic cells are recognized as key determinants of germ cell fate. Notably, the loss of germ cells in zebrafish leads to masculinization, implying that germ cells harbor an intrinsic feminization signal. However, the specific signal triggering oogenesis in zebrafish remains unclear. In the present study, we identified foxl2l as an oocyte progenitor-specific gene essential for initiating oogenesis in germ cells. Results showed that foxl2l-knockout zebrafish bypassed the juvenile ovary stage and exclusively developed into fertile males. Further analysis revealed that loss of foxl2l hindered the initiation of oocyte-specific meiosis and prevented entry into oogenesis, leading to premature spermatogenesis during early gonadal development. Furthermore, while mutation of the pro-male gene dmrt1 led to fertile female differentiation, simultaneous disruption of foxl2l in dmrt1 mutants completely blocked oogenesis, with a large proportion of germ cells arrested as germline stem cells, highlighting the crucial role of foxl2l in oogenesis. Overall, this study highlights the unique function of foxl2l as a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.
期刊介绍:
Established in 1980, Zoological Research (ZR) is a bimonthly publication produced by Kunming Institute of Zoology, the Chinese Academy of Sciences, and the China Zoological Society. It publishes peer-reviewed original research article/review/report/note/letter to the editor/editorial in English on Primates and Animal Models, Conservation and Utilization of Animal Resources, and Animal Diversity and Evolution.