foxl2l 是斑马鱼卵子发生的生殖细胞内在守门员。

IF 4 1区 生物学 Q1 ZOOLOGY
Zhiqin Ren, Ding Ye, Naike Su, Chaofan Wang, Lijia He, Houpeng Wang, Mudan He, Yonghua Sun
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引用次数: 0

摘要

斑马鱼是研究生殖细胞生物学和生殖过程的重要模式生物。AB 株斑马鱼被认为是一种多基因性别决定系统,大多数雄性斑马鱼最初发育出幼年卵巢,然后才开始雄性命运。在染色体性别决定的物种中,性腺体细胞被认为是决定生殖细胞命运的关键因素。值得注意的是,斑马鱼失去生殖细胞会导致男性化,这意味着生殖细胞蕴藏着内在的女性化信号。然而,触发斑马鱼卵子发生的特定信号仍不清楚。在本研究中,我们发现 foxl2l 是卵母细胞祖细胞特异性基因,对启动生殖细胞的卵子发生至关重要。结果表明,敲除 foxl2l 的斑马鱼绕过了幼年卵巢阶段,完全发育成可育雄鱼。进一步的分析表明,foxl2l的缺失阻碍了卵母细胞特异性减数分裂的启动,阻止了卵子的发生,导致性腺发育早期精子发生过早。此外,虽然突变促雄性基因dmrt1会导致可育的雌性分化,但在dmrt1突变体中同时破坏foxl2l会完全阻断卵子的发生,大部分生殖细胞会作为生殖干细胞停止发育,这突显了foxl2l在卵子发生过程中的关键作用。总之,本研究强调了 foxl2l 在斑马鱼卵子发生过程中作为生殖细胞内在看门人的独特功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
foxl2l is a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.

Zebrafish serve as a valuable model organism for studying germ cell biology and reproductive processes. The AB strain of zebrafish is proposed to exhibit a polygenic sex determination system, where most males initially develop juvenile ovaries before committing to male fate. In species with chromosomal sex determination, gonadal somatic cells are recognized as key determinants of germ cell fate. Notably, the loss of germ cells in zebrafish leads to masculinization, implying that germ cells harbor an intrinsic feminization signal. However, the specific signal triggering oogenesis in zebrafish remains unclear. In the present study, we identified foxl2l as an oocyte progenitor-specific gene essential for initiating oogenesis in germ cells. Results showed that foxl2l-knockout zebrafish bypassed the juvenile ovary stage and exclusively developed into fertile males. Further analysis revealed that loss of foxl2l hindered the initiation of oocyte-specific meiosis and prevented entry into oogenesis, leading to premature spermatogenesis during early gonadal development. Furthermore, while mutation of the pro-male gene dmrt1 led to fertile female differentiation, simultaneous disruption of foxl2l in dmrt1 mutants completely blocked oogenesis, with a large proportion of germ cells arrested as germline stem cells, highlighting the crucial role of foxl2l in oogenesis. Overall, this study highlights the unique function of foxl2l as a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.

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来源期刊
Zoological Research
Zoological Research Medicine-General Medicine
CiteScore
7.60
自引率
10.20%
发文量
1937
审稿时长
8 weeks
期刊介绍: Established in 1980, Zoological Research (ZR) is a bimonthly publication produced by Kunming Institute of Zoology, the Chinese Academy of Sciences, and the China Zoological Society. It publishes peer-reviewed original research article/review/report/note/letter to the editor/editorial in English on Primates and Animal Models, Conservation and Utilization of Animal Resources, and Animal Diversity and Evolution.
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