使用流式细胞仪和成像流式细胞仪评估小鼠细胞双层接合及其后续影响的方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-09-21 DOI:10.1016/j.xpro.2024.103152

Yuval Shapir Itai, Ziv Porat, Rony Dahan

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引用次数: 0

摘要

两种免疫细胞之间或免疫细胞与癌细胞之间的物理相互作用在形成肿瘤微环境中的免疫反应方面起着重要作用，因此成为双特异性接合剂的主要治疗目标。在此，我们介绍一种使用流式细胞仪和成像流式细胞仪评估小鼠细胞双特异性接合及其后续效应的方案。我们描述了在细胞-细胞界面上识别双特异性细胞接合抗体、双特异性定量以及描述细胞蛋白形态和双特异性内过程的步骤。有关该方案使用和执行的完整细节，请参阅 Shapir Itai 等人的文章1。

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Protocol for assessing murine cell doublet engagement and subsequent effects using flow cytometry and imaging flow cytometry.

Physical interactions between two immune cells or between immune and cancer cells play a major role in shaping the immune response in the tumor microenvironment, making them prime therapeutic targets for bispecific engagers. Here, we present a protocol for assessing murine cell doublet engagement and subsequent effects using flow cytometry and imaging flow cytometry. We describe steps for identifying bispecific cell engager antibodies at the cell-cell interface, doublet quantification, and characterizing cellular protein morphology and processes within the doublet. For complete details on the use and execution of this protocol, please refer to Shapir Itai et al.¹.

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