犬原发性角膜鳞状细胞癌的活体共聚焦显微镜特征。

IF 1.7 4区 农林科学 Q2 VETERINARY SCIENCES
Callie M Rogers, Kelly E Knickelbein, Erin M Scott, Eric C Ledbetter
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引用次数: 0

摘要

目的:描述原发性角膜鳞状细胞癌(SCC)犬的体内共聚焦显微镜(IVCM)检查结果:描述原发性角膜鳞状细胞癌(SCC)犬的体内共聚焦显微镜(IVCM)检查结果:八只患有原发性角膜鳞状细胞癌的狗:手术干预前,使用改良海德堡视网膜断层显微镜和罗斯托克角膜模块对经组织病理学诊断为原发性角膜 SCC 的狗进行检查。IVCM 检查结果与眼科检查和诊断测试结果中的临床细节相关联:对 8 只患有单侧原发性角膜 SCC 的狗的 8 只眼睛进行了检查。通过 IVCM 检查,角膜病变的特征是上皮细胞异常,呈多角形且增大,形态差异明显,个别角膜内有异形细胞。异常细胞显示出不同的反射率,但大多数细胞边界反射率高,细胞质反射率中等。偶尔也能观察到明显的弥漫性高反射细胞,可能代表角质化和角化不良。在 IVCM 上,肿瘤细胞呈条状、簇状和片状,肿瘤细胞巢经常侵入角膜基质。角蛋白珍珠是一种常见的病变,表现为新生上皮细胞围绕同心层的高反光无定形物质形成的环状漩涡,位于暗色的囊状空间内。所有肿瘤中都有丰富的血管和散在的白细胞。所有病例的 IVCM 分析结果与组织病理学结果在形态学上高度一致:在 IVCM 检查中,原发性角膜 SCC 的特征与肿瘤切片组织病理学检查中的特征相似,包括增大和多形性鳞状上皮细胞、异形细胞增多、角蛋白珍珠形成和密集的血管。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vivo confocal microscopic features of canine primary corneal squamous cell carcinoma.

Objective: To describe the in vivo confocal microscopy (IVCM) findings in dogs with primary corneal squamous cell carcinoma (SCC).

Animals: Eight dogs with primary corneal SCC.

Procedures: Dogs diagnosed with primary corneal SCC by histopathology were examined with a modified Heidelberg Retina tomograph and Rostock Cornea Module prior to surgical intervention. The findings from the IVCM examination were correlated with clinical details from ophthalmic examinations and diagnostic test results.

Results: Eight eyes from eight dogs with unilateral primary corneal SCC were examined. Corneal lesions were characterized with IVCM by abnormal epithelial cells that were polygonal and enlarged with marked morphological variability and anisocytosis within individual corneas. The abnormal cells displayed variable reflectivity, but most had highly reflective cellular borders and moderately reflective cytoplasm. Cells that were markedly and diffusely hyperreflective were also occasionally observed possibly representing keratinization and dyskeratosis. On IVCM, neoplastic cells were grouped into cords, clusters, and sheets, and nests of neoplastic cells often invaded the underlying corneal stroma. Keratin pearls were a frequent finding and appeared as circular whirls of neoplastic epithelial cells arranged around concentric layers of hyperreflective amorphous material within a dark cyst-like space. Abundant blood vessels and scattered leukocytes were present in all tumors. There was a high degree of morphologic agreement between IVCM analysis and histopathological findings in all cases.

Conclusions: The distinguishing features of primary corneal SCC during IVCM examine were similar to those seen in histopathologic examination of tumor sections, including enlarged and pleomorphic squamous epithelial cells, anisocytosis, keratin pearl formation, and dense vascularization.

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来源期刊
Veterinary ophthalmology
Veterinary ophthalmology 农林科学-兽医学
CiteScore
2.70
自引率
37.50%
发文量
82
审稿时长
12-24 weeks
期刊介绍: Veterinary Ophthalmology is a peer-reviewed, international journal that welcomes submission of manuscripts directed towards academic researchers of veterinary ophthalmology, specialists and general practitioners with a strong ophthalmology interest. Articles include those relating to all aspects of: Clinical and investigational veterinary and comparative ophthalmology; Prospective and retrospective studies or reviews of naturally occurring ocular disease in veterinary species; Experimental models of both animal and human ocular disease in veterinary species; Anatomic studies of the animal eye; Physiological studies of the animal eye; Pharmacological studies of the animal eye.
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