小母牛对实验性犬新孢子虫感染的细胞介导免疫反应的品种差异。

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2024-09-16 DOI:10.1016/j.vetimm.2024.110828

F. Fiorani , B. Dallard , F.A. Cheuquepán , E. Sosa , A.M. Pardo , I. Gual , E.L. Morrell , M.S. Marín , S. Quintana , G.J. Cantón , B.S. Valentini , I.E. Echaide , S.M. Torioni , E.R. Cobo , P.M. Corva , D.P. Moore

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引用次数: 0

摘要

原生寄生虫犬新孢子虫会导致受感染的牛流产，而其他牛则没有症状。宿主免疫对牛新孢子虫病的结果起着关键作用。尽管进行了广泛的研究，但在治疗和预防措施方面仍存在巨大差距，而且目前还没有有效的疫苗。肉牛和奶牛都可能因牛新孢子虫病而流产，但累积的证据表明，奶牛品种的易感性高于肉牛品种。已经证实，对 N. caninum 感染的反应主要涉及细胞介导的免疫反应（CMIR），由 1 型 T 辅助细胞（Th1）和特定的细胞因子调节。延迟型超敏反应（DTH）皮试已被用于测量家畜产生 CMIR 的能力，以培育抗病力，并作为诊断多种疾病的一种方法。在这项研究中，我们评估了荷斯坦母牛（一种经过严格选育的奶牛品种）和阿根廷克里奥尔母牛（一种经过最少遗传选育的肉牛品种）之间通过金线梭菌诱导的 DTH 皮肤试验所引发的免疫反应，以评估实验性金线梭菌感染后 CMIR 的差异。通过皮褶厚度、组织学和免疫分子分析测试的免疫反应显示了不同品种之间的差异。我们的研究发现，与荷斯坦小母牛相比，阿根廷克里奥尔小母牛的CMIR有所增加。在 DTH 皮肤测试部位观察到关键细胞因子的基因表达存在差异。阿根廷克里奥尔小母牛的 IFN-γ、IL-12、IL-10 和 IL-4 表达升高，而荷斯坦小母牛仅 IL-17 表达升高。这一发现强调了对新孢子虫病反应的遗传多样性，可用于牛群抗病育种策略。

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Breed variability in the cellular mediated immune response to experimental Neospora caninum infection in heifers

Protozoan parasite Neospora caninum causes abortion in infected cattle while others remain asymptomatic. Host immunity plays a critical role in the outcome of bovine neosporosis. Despite extensive research, there is a critical gap in therapeutic and preventive measures, and no effective vaccines are available. Both beef and dairy cattle can suffer from N. caninum-induced abortions, but cumulative evidence suggests a breed susceptibility being higher in dairy compared with beef breeds. It has been established that the response to N. caninum infection primarily involves a cell-mediated immune response (CMIR) regulated by T-helper type 1 (Th1) cells and specific cytokines. The delayed-type hypersensitivity (DTH) skin test has been used to measure the ability of livestock to generate CMIR, in the context of breeding for disease resistance and as a method for diagnosis of several diseases. In this study, we evaluated the immune response triggered by an N. caninum-induced DTH skin test between Holstein – a dairy breed intensively selected- and Argentinean Creole heifers – a beef breed with minimal genetic selection- to assess differences in CMIR following experimental N. caninum infection. The immune response, measured through skinfold thickness and histological and immune molecular analysis, revealed variations between the breeds. Our study found an increased CMIR in Argentinean Creole heifers compared to Holstein heifers. Differential gene expression of key cytokines was observed at the DTH skin test site. Argentinean Creole heifers exhibited elevated IFN-γ, IL-12, IL-10, and IL-4, while Holstein heifers only showed higher expression of IL-17. This finding could underscore genetic diversity in response to neosporosis, which could be used in breeding cattle strategies for disease resistance in cattle populations.

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.