异体造血细胞移植中移植后微粒的监测和定量。

IF 1.6 4区医学 Q4 IMMUNOLOGY

Transplant immunology Pub Date : 2024-09-20 DOI:10.1016/j.trim.2024.102133

A. Xagorari , M. Iskas , V. Papadopoulos , C. Dimosthenous , E. Gavriilaki , D. Bougiouklis , I. Sakellari , D. Sotiropoulos

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引用次数: 0

摘要

背景：异基因造血干细胞移植（allo-HCT）是治疗各种血液相关疾病（包括血液恶性肿瘤和遗传性疾病）的一种治愈性疗法。该疗法的成功与否取决于调理方案的疗效以及移植物能否正常移植和发挥作用。微颗粒（MPs）是由受刺激、凋亡或活化的细胞产生的小囊泡，参与生理和病理过程。然而，人们对 MPs 对异体肝移植的影响仍知之甚少：本研究旨在调查异体肝移植后移植物和患者血浆中不同类型细胞的 MPs 存在情况及其与各种参数的关系。我们测量了移植后第 0 天到第 60 天移植物和患者血浆中来自 CD34+、CD56+、CD3+、CD19+ 和 CD33+ 细胞的 MPs。从血浆中分离出 MPs，并通过流式细胞术进行定量分析：MP水平随时间变化。值得注意的是，CD34+ MP水平与中性粒细胞和血小板的早期和晚期移植有关。此外，移植物第0天和第+4天患者血浆中CD34+和CD56+ MP水平高与晚期移植有关，而移植物和第+4天患者血浆中CD33+ MP水平高与早期移植有关。治疗方案会影响+14天时的CD19+ MP水平，移植后30天CD34+、CD56+和CD19+ MP的数量与急性移植物抗宿主病相关：这些研究结果表明，来自造血细胞的 MPs 可能在异体肝移植患者的临床病程中发挥重要作用。

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Post-transplantation monitoring and quantitation of microparticles in allogeneic hematopoietic cell transplantation

Background

Allogeneic hematopoietic stem cell transplantation (allo-HCT) represents a curative treatment for various blood-related disorders, including hematological malignancies and genetic disorders. The success of this procedure hinges on the efficacy of the conditioning regimen and the graft's ability to engraft and function properly. Microparticles (MPs), small vesicles produced from stimulated, apoptotic, or activated cells, are involved in both physiological and pathological processes. However, the impact of MPs on allo-HCT remains poorly understood.

Objectives

This study aimed to investigate the presence of MPs from different cell types in grafts and patient plasma after allo-HCT, as well as their association with various parameters. We measured MPs from CD34+, CD56+, CD3+, CD19+, and CD33+ cells in grafts and patient plasma from day 0 to day 60 after transplantation.

Methods

224 blood samples were collected from 19 consecutive allo –HCT recipients at 0, +4, +14,+30 and + 60 day as well as from their grafts. MPs isolated from the plasma and quantified by flow cytometry analysis.

Results

MP levels varied over time. Notably, CD34+ MP levels were linked to both early and late engraftment of neutrophils and platelets. Furthermore, grafts with high CD34+ and CD56+ MP levels in patient plasma on days 0 and + 4 were associated with late engraftment, whereas high CD33+ MP levels in both graft and patient plasma on day +4 were associated with early engraftment. Conditioning regimen affected CD19+ MP levels at day +14, and the number of CD34+, CD56+, and CD19+ MPs 30 days after transplantation was correlated with acute graft-versus-host disease.

Conclusion

These findings suggest that MPs derived from hematopoietic cells may play a significant role in the clinical course of patients following allo-HCT.

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来源期刊

Transplant immunology 医学-免疫学

CiteScore

2.10

自引率

13.30%

发文量

198

审稿时长

48 days

期刊介绍： Transplant Immunology will publish up-to-date information on all aspects of the broad field it encompasses. The journal will be directed at (basic) scientists, tissue typers, transplant physicians and surgeons, and research and data on all immunological aspects of organ-, tissue- and (haematopoietic) stem cell transplantation are of potential interest to the readers of Transplant Immunology. Original papers, Review articles and Hypotheses will be considered for publication and submitted manuscripts will be rapidly peer-reviewed and published. They will be judged on the basis of scientific merit, originality, timeliness and quality.