大容量全自动细胞重建生成植物组织细胞图谱。

IF 10 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Plant Cell Pub Date : 2024-09-16 DOI:10.1093/plcell/koae250
Zijian Hu, Jiazheng Liu, Shiya Shen, Weiqian Wu, Jingbin Yuan, Weiwei Shen, Lingyu Ma, Guangchao Wang, Shunyao Yang, Xiuping Xu, Yaning Cui, Zhenchen Li, Lijun Shen, Linlin Li, Jiahui Bian, Xi Zhang, Hua Han, Jinxing Lin
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引用次数: 0

摘要

单个细胞的几何形状和排列在塑造器官功能方面发挥着作用。然而,在厘米尺度的植物器官中分析多细胞特征和探索其连接组仍然具有挑战性。在此,我们建立了一套名为 "大体积全自动细胞重建(LVACR)"的框架,可用于探索植物组织中的三维(3D)细胞学特征和细胞连通性。通过基准测试,我们的框架在细胞分割和聚集方面表现出了卓越的效率,成功地解决了光片荧光显微镜(LSFM)成像带来的固有挑战。利用 LVACR,我们成功建立了不同植物组织的细胞图谱。细胞形态分析表明,不同杨树(P. simonii Carr. 和 P. canadensis Moench.此外,LVACR 在时空上显示出细胞增殖的初始爆发,并伴随着芽尖分生组织发育早期的形态转变。在随后的发育过程中,细胞分化产生了各向异性特征,从而形成了各种细胞形状。总之,我们的研究结果为了解多细胞生物的精确空间排列和细胞行为提供了宝贵的见解,从而加深了我们对植物生长和分化的复杂过程的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Large-volume fully automated cell reconstruction generates a cell atlas of plant tissues.

The geometric shape and arrangement of individual cells play a role in shaping organ functions. However, analyzing multicellular features and exploring their connectomes in centimeter-scale plant organs remain challenging. Here, we established a set of frameworks named Large-Volume Fully Automated Cell Reconstruction (LVACR), enabling the exploration of three-dimensional (3D) cytological features and cellular connectivity in plant tissues. Through benchmark testing, our framework demonstrated superior efficiency in cell segmentation and aggregation, successfully addressing the inherent challenges posed by light sheet fluorescence microscopy (LSFM) imaging. Using LVACR, we successfully established a cell atlas of different plant tissues. Cellular morphology analysis revealed differences of cell clusters and shapes in between different poplar (P. simonii Carr. and P. canadensis Moench.) seeds, whereas topological analysis revealed that they maintained conserved cellular connectivity. Furthermore, LVACR spatiotemporally demonstrated an initial burst of cell proliferation, accompanied by morphological transformations at an early stage in developing the shoot apical meristem. During subsequent development, cell differentiation produced anisotropic features, thereby resulting in various cell shapes. Overall, our findings provided valuable insights into the precise spatial arrangement and cellular behavior of multicellular organisms, thus enhancing our understanding of the complex processes underlying plant growth and differentiation.

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来源期刊
Plant Cell
Plant Cell 生物-生化与分子生物学
CiteScore
16.90
自引率
5.20%
发文量
337
审稿时长
2.4 months
期刊介绍: Title: Plant Cell Publisher: Published monthly by the American Society of Plant Biologists (ASPB) Produced by Sheridan Journal Services, Waterbury, VT History and Impact: Established in 1989 Within three years of publication, ranked first in impact among journals in plant sciences Maintains high standard of excellence Scope: Publishes novel research of special significance in plant biology Focus areas include cellular biology, molecular biology, biochemistry, genetics, development, and evolution Primary criteria: articles provide new insight of broad interest to plant biologists and are suitable for a wide audience Tenets: Publish the most exciting, cutting-edge research in plant cellular and molecular biology Provide rapid turnaround time for reviewing and publishing research papers Ensure highest quality reproduction of data Feature interactive format for commentaries, opinion pieces, and exchange of information in review articles, meeting reports, and insightful overviews.
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