钙调蛋白能与质膜细胞膜小叶的脂质结合吗?

IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Open Biology Pub Date : 2024-09-01 Epub Date: 2024-09-18 DOI:10.1098/rsob.240067
Federica Scollo, Carmelo Tempra, Hüseyin Evci, Miguel Riopedre-Fernandez, Agnieszka Olżyńska, Matti Javanainen, Arunima Uday, Marek Cebecauer, Lukasz Cwiklik, Hector Martinez-Seara, Pavel Jungwirth, Piotr Jurkiewicz, Martin Hof
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引用次数: 0

摘要

钙调蛋白(CaM)是真核细胞中无处不在的钙敏感信使。以前的研究表明,CaM 对不同的脂质分子(包括 CaM 结合蛋白上的脂质分子)具有亲和力。这些事实,再加上我们观察到 CaM 在细胞膜钙增加时聚集在 HeLa 细胞富含膜的突起中,促使我们系统地寻找 CaM 与模拟质膜细胞膜小叶的模型脂膜的非介导相互作用。一系列实验技术和分子动力学模拟明确证明,钙离子存在时,CaM 与脂质双层膜相互作用。磷脂酰丝氨酸(PS)和磷脂酰乙醇胺(PE)是 CaM 与膜相互作用的关键。钙诱导 CaM 发生重要的构象重排,但钙与 PS 的头基团结合也会中和膜表面负电荷。更有趣的是,PE 起着双重作用--它不仅能与 CaM 形成氢键,还能破坏脂质双分子层的稳定性,增加疏水酰基链与相互作用蛋白质的接触。我们的研究结果表明,当细胞内钙浓度升高时,CaM 和细胞膜的胞浆小叶会发生功能性连接。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Can calmodulin bind to lipids of the cytosolic leaflet of plasma membranes?

Calmodulin (CaM) is a ubiquitous calcium-sensitive messenger in eukaryotic cells. It was previously shown that CaM possesses an affinity for diverse lipid moieties, including those found on CaM-binding proteins. These facts, together with our observation that CaM accumulates in membrane-rich protrusions of HeLa cells upon increased cytosolic calcium, motivated us to perform a systematic search for unmediated CaM interactions with model lipid membranes mimicking the cytosolic leaflet of plasma membranes. A range of experimental techniques and molecular dynamics simulations prove unambiguously that CaM interacts with lipid bilayers in the presence of calcium ions. The lipids phosphatidylserine (PS) and phosphatidylethanolamine (PE) hold the key to CaM-membrane interactions. Calcium induces an essential conformational rearrangement of CaM, but calcium binding to the headgroup of PS also neutralizes the membrane negative surface charge. More intriguingly, PE plays a dual role-it not only forms hydrogen bonds with CaM, but also destabilizes the lipid bilayer increasing the exposure of hydrophobic acyl chains to the interacting proteins. Our findings suggest that upon increased intracellular calcium concentration, CaM and the cytosolic leaflet of cellular membranes can be functionally connected.

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来源期刊
Open Biology
Open Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
10.00
自引率
1.70%
发文量
136
审稿时长
6-12 weeks
期刊介绍: Open Biology is an online journal that welcomes original, high impact research in cell and developmental biology, molecular and structural biology, biochemistry, neuroscience, immunology, microbiology and genetics.
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