Zahira Abdljabbar Al-Zuhairi, Esraa Taher Muslim, Orooba Meteab Faja, Ziad M Alkhozai, Basima Jasim Mohammed
{"title":"奶酪样本中与金黄色葡萄球菌相关的肠毒素基因的分子鉴定。","authors":"Zahira Abdljabbar Al-Zuhairi, Esraa Taher Muslim, Orooba Meteab Faja, Ziad M Alkhozai, Basima Jasim Mohammed","doi":"10.5455/OVJ.2024.v14.i8.36","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Dairy products are considered some important sources of various nutritional compounds; however, pathogenic bacterial growth is a critical destructive factor to these products leading to consumer health and system financial crises.</p><p><strong>Aim: </strong>The current study was carried out to identify if there is any presence of <i>Staphylococcus aureus</i>-related enterotoxin genes in cheese samples.</p><p><strong>Methods: </strong>The research included the collection of 35 samples. The samples passed through conventional cultivation processes and a PCR method to detect the presence of <i>icaA</i>, <i>sea</i>, <i>hla</i>, and <i>fnbA</i> enterotoxin genes in these samples.</p><p><strong>Results: </strong>The conventional identification revealed the growth of <i>S</i>. <i>aureus</i> from the cheese samples. The PCR findings recorded the presence of the <i>icaA</i>, <i>sea</i>, <i>hla</i>, and <i>fnbA</i> in 31 (88.5%), 27 (77%), 19 (54%), and 12 (34%), respectively, of cheese samples. The sequencing revealed close similarities with global isolates, which reached up to 98.5% of identity.</p><p><strong>Conclusion: </strong>The current results indicate the presence of enterotoxin genes of <i>S. aureus</i> in high rates in the dairy products examined, which reveals critical problems of food safety due to the possible presence of enterotoxins in consumer dairy products.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415922/pdf/","citationCount":"0","resultStr":"{\"title\":\"Molecular identification of <i>Staphylococcus aureus</i>-related enterotoxin genes in cheese samples.\",\"authors\":\"Zahira Abdljabbar Al-Zuhairi, Esraa Taher Muslim, Orooba Meteab Faja, Ziad M Alkhozai, Basima Jasim Mohammed\",\"doi\":\"10.5455/OVJ.2024.v14.i8.36\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Dairy products are considered some important sources of various nutritional compounds; however, pathogenic bacterial growth is a critical destructive factor to these products leading to consumer health and system financial crises.</p><p><strong>Aim: </strong>The current study was carried out to identify if there is any presence of <i>Staphylococcus aureus</i>-related enterotoxin genes in cheese samples.</p><p><strong>Methods: </strong>The research included the collection of 35 samples. The samples passed through conventional cultivation processes and a PCR method to detect the presence of <i>icaA</i>, <i>sea</i>, <i>hla</i>, and <i>fnbA</i> enterotoxin genes in these samples.</p><p><strong>Results: </strong>The conventional identification revealed the growth of <i>S</i>. <i>aureus</i> from the cheese samples. The PCR findings recorded the presence of the <i>icaA</i>, <i>sea</i>, <i>hla</i>, and <i>fnbA</i> in 31 (88.5%), 27 (77%), 19 (54%), and 12 (34%), respectively, of cheese samples. The sequencing revealed close similarities with global isolates, which reached up to 98.5% of identity.</p><p><strong>Conclusion: </strong>The current results indicate the presence of enterotoxin genes of <i>S. aureus</i> in high rates in the dairy products examined, which reveals critical problems of food safety due to the possible presence of enterotoxins in consumer dairy products.</p>\",\"PeriodicalId\":19531,\"journal\":{\"name\":\"Open Veterinary Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415922/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Open Veterinary Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/OVJ.2024.v14.i8.36\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/31 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/OVJ.2024.v14.i8.36","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/31 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Molecular identification of Staphylococcus aureus-related enterotoxin genes in cheese samples.
Background: Dairy products are considered some important sources of various nutritional compounds; however, pathogenic bacterial growth is a critical destructive factor to these products leading to consumer health and system financial crises.
Aim: The current study was carried out to identify if there is any presence of Staphylococcus aureus-related enterotoxin genes in cheese samples.
Methods: The research included the collection of 35 samples. The samples passed through conventional cultivation processes and a PCR method to detect the presence of icaA, sea, hla, and fnbA enterotoxin genes in these samples.
Results: The conventional identification revealed the growth of S. aureus from the cheese samples. The PCR findings recorded the presence of the icaA, sea, hla, and fnbA in 31 (88.5%), 27 (77%), 19 (54%), and 12 (34%), respectively, of cheese samples. The sequencing revealed close similarities with global isolates, which reached up to 98.5% of identity.
Conclusion: The current results indicate the presence of enterotoxin genes of S. aureus in high rates in the dairy products examined, which reveals critical problems of food safety due to the possible presence of enterotoxins in consumer dairy products.
期刊介绍：
Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
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