Neta Ambar, Mary I Thurber, Fabiano Montiani-Ferreira, Carolyn Cray
{"title":"评估管理环境中健康长臂猿的急性期蛋白和蛋白电泳。","authors":"Neta Ambar, Mary I Thurber, Fabiano Montiani-Ferreira, Carolyn Cray","doi":"10.1638/2023-0101","DOIUrl":null,"url":null,"abstract":"<p><p>Acute phase proteins (APP) and protein electrophoresis (EPH) offer crucial insights into inflammation and overall health in various species. In this study, we validated serum amyloid A (SAA) and C-reactive protein (CRP) reagents for use with serum samples from gibbons (Hylobatidae, <i>n</i> = 50), spanning five species across four gibbon genera: eastern hoolock (<i>Hoolock leuconedys</i>), Javan (<i>Hylobates moloch</i>), pileated (<i>Hylobates pileatus</i>), siamang (<i>Symphalangus syndactylus</i>), and white-cheeked (<i>Nomascus leucogenys</i>). Preliminary reference intervals (<i>n</i> = 50) were calculated for SAA (1.8-48.1 mg/L), CRP (0.1-11.1 mg/L), and EPH via capillary zone electrophoresis, in healthy gibbons. Comparing clinically normal (<i>n</i> = 38) and abnormal (<i>n</i> = 12) individuals, significant differences were observed in the albumin/globulin ratio (<i>P</i> = 0.0003), prealbumin (<i>P</i> = 0.0345), and albumin (<i>P</i> = 0.0094), with abnormal individuals exhibiting statistically significantly higher γ-globulins (<i>P</i> = 0.0224), SAA (<i>P</i> = 0.0001), and CRP (<i>P</i> = 0.0003). Despite significant chromosomal rearrangements among different gibbon species, we found no statistically significant differences of SAA and CRP levels across species. However, some differences between species were observed in EPH fractions. This study presents the first report of the evaluation of APP and EPH in gibbons, underscoring the potential use of these biomarkers in gibbon health monitoring. Further research with larger sample sizes of both normal and abnormal gibbons is recommended to solidify the clinical utility of these biomarkers in these species.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"ASSESSMENT OF ACUTE PHASE PROTEINS AND PROTEIN ELECTROPHORESIS IN HEALTHY GIBBONS (HYLOBATIDAE) IN MANAGED SETTINGS.\",\"authors\":\"Neta Ambar, Mary I Thurber, Fabiano Montiani-Ferreira, Carolyn Cray\",\"doi\":\"10.1638/2023-0101\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Acute phase proteins (APP) and protein electrophoresis (EPH) offer crucial insights into inflammation and overall health in various species. In this study, we validated serum amyloid A (SAA) and C-reactive protein (CRP) reagents for use with serum samples from gibbons (Hylobatidae, <i>n</i> = 50), spanning five species across four gibbon genera: eastern hoolock (<i>Hoolock leuconedys</i>), Javan (<i>Hylobates moloch</i>), pileated (<i>Hylobates pileatus</i>), siamang (<i>Symphalangus syndactylus</i>), and white-cheeked (<i>Nomascus leucogenys</i>). Preliminary reference intervals (<i>n</i> = 50) were calculated for SAA (1.8-48.1 mg/L), CRP (0.1-11.1 mg/L), and EPH via capillary zone electrophoresis, in healthy gibbons. Comparing clinically normal (<i>n</i> = 38) and abnormal (<i>n</i> = 12) individuals, significant differences were observed in the albumin/globulin ratio (<i>P</i> = 0.0003), prealbumin (<i>P</i> = 0.0345), and albumin (<i>P</i> = 0.0094), with abnormal individuals exhibiting statistically significantly higher γ-globulins (<i>P</i> = 0.0224), SAA (<i>P</i> = 0.0001), and CRP (<i>P</i> = 0.0003). Despite significant chromosomal rearrangements among different gibbon species, we found no statistically significant differences of SAA and CRP levels across species. However, some differences between species were observed in EPH fractions. This study presents the first report of the evaluation of APP and EPH in gibbons, underscoring the potential use of these biomarkers in gibbon health monitoring. Further research with larger sample sizes of both normal and abnormal gibbons is recommended to solidify the clinical utility of these biomarkers in these species.</p>\",\"PeriodicalId\":17667,\"journal\":{\"name\":\"Journal of Zoo and Wildlife Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Zoo and Wildlife Medicine\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1638/2023-0101\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Zoo and Wildlife Medicine","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1638/2023-0101","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
ASSESSMENT OF ACUTE PHASE PROTEINS AND PROTEIN ELECTROPHORESIS IN HEALTHY GIBBONS (HYLOBATIDAE) IN MANAGED SETTINGS.
Acute phase proteins (APP) and protein electrophoresis (EPH) offer crucial insights into inflammation and overall health in various species. In this study, we validated serum amyloid A (SAA) and C-reactive protein (CRP) reagents for use with serum samples from gibbons (Hylobatidae, n = 50), spanning five species across four gibbon genera: eastern hoolock (Hoolock leuconedys), Javan (Hylobates moloch), pileated (Hylobates pileatus), siamang (Symphalangus syndactylus), and white-cheeked (Nomascus leucogenys). Preliminary reference intervals (n = 50) were calculated for SAA (1.8-48.1 mg/L), CRP (0.1-11.1 mg/L), and EPH via capillary zone electrophoresis, in healthy gibbons. Comparing clinically normal (n = 38) and abnormal (n = 12) individuals, significant differences were observed in the albumin/globulin ratio (P = 0.0003), prealbumin (P = 0.0345), and albumin (P = 0.0094), with abnormal individuals exhibiting statistically significantly higher γ-globulins (P = 0.0224), SAA (P = 0.0001), and CRP (P = 0.0003). Despite significant chromosomal rearrangements among different gibbon species, we found no statistically significant differences of SAA and CRP levels across species. However, some differences between species were observed in EPH fractions. This study presents the first report of the evaluation of APP and EPH in gibbons, underscoring the potential use of these biomarkers in gibbon health monitoring. Further research with larger sample sizes of both normal and abnormal gibbons is recommended to solidify the clinical utility of these biomarkers in these species.
期刊介绍:
The Journal of Zoo and Wildlife Medicine (JZWM) is considered one of the major sources of information on the biology and veterinary aspects in the field. It stems from the founding premise of AAZV to share zoo animal medicine experiences. The Journal evolved from the long history of members producing case reports and the increased publication of free-ranging wildlife papers.
The Journal accepts manuscripts of original research findings, case reports in the field of veterinary medicine dealing with captive and free-ranging wild animals, brief communications regarding clinical or research observations that may warrant publication. It also publishes and encourages submission of relevant editorials, reviews, special reports, clinical challenges, abstracts of selected articles and book reviews. The Journal is published quarterly, is peer reviewed, is indexed by the major abstracting services, and is international in scope and distribution.
Areas of interest include clinical medicine, surgery, anatomy, radiology, physiology, reproduction, nutrition, parasitology, microbiology, immunology, pathology (including infectious diseases and clinical pathology), toxicology, pharmacology, and epidemiology.