慢性心力衰竭患者 GPX3 Promoter 中甲基化水平的动态变化及其与主要临床参数的关系

IF 3.7 3区医学 Q2 NUTRITION & DIETETICS

Journal of Nutrition Pub Date : 2024-11-01 DOI:10.1016/j.tjnut.2024.08.033

Yanmei Liu , Xu Zhao , Chuanyong Qu , Mengli Chen , Rongqiang Zhang

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引用次数: 0

摘要

研究目的本研究调查了慢性心力衰竭（CHF）患者谷胱甘肽过氧化物酶 3（GPX3）启动子区域甲基化水平的变化。研究收集了 20 名慢性心力衰竭患者和 20 名健康人的外周血样本进行分析：方法：使用基质辅助激光解吸电离飞行时间质谱对 GPX3 启动子区域内 11 个 CpG 位点的甲基化水平进行量化：结果发现，与对照组相比，CHF 患者 GPX3_FA10_CpG_24 位点的甲基化水平明显升高（P < 0.05）。此外，该位点的甲基化水平与血清载脂蛋白 A-1、D-二聚体、氯(Cl)、钾(K)和钠(Na)等主要临床指标之间存在非线性剂量反应关系（P < 0.05）：这些研究结果表明，GPX3 启动子的异常甲基化可能会影响血脂、凝血和电解质等生理功能，从而影响疾病的进展。我们有必要进一步研究GPX3启动子甲基化在CHF发病机制中的作用，从而为CHF的预防、诊断和治疗提供有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Dynamics of Methylation Concentrations in Glutathione Peroxidase 3 Promoter from Patients with Chronic Heart Failure and Their Association with Key Clinical Parameters

Objective

This study investigated changes in methylation concentrations within the glutathione peroxidase 3 (GPX3) promoter region among patients diagnosed with chronic heart failure (CHF). Peripheral blood samples were collected from 20 CHF patients and 20 healthy individuals for analysis.

Methods

Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, methylation concentrations of 11 CpG sites within the GPX3 promoter region were quantified.

Results

Results showed a significant increase in methylation at the GPX3_FA10_CpG_24 site in patients with CHF compared with the control group (P < 0.05). Furthermore, a nonlinear dose–response relationship was observed between methylation concentrations at this site and key clinical parameters including serum apolipoprotein A-1, D-dimer, chlorine, potassium, and sodium (Na) (P < 0.05).

Conclusions

These findings suggest that aberrant methylation of the GPX3 promoter may impact disease progression by influencing physiological functions such as blood lipids, coagulation, and electrolytes. Further investigations are warranted to elucidate the role of GPX3 promoter methylation in CHF pathogenesis, potentially contributing valuable insights for its prevention, diagnosis, and treatment.

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来源期刊

Journal of Nutrition 医学-营养学

CiteScore

7.60

自引率

4.80%

发文量

260

审稿时长

39 days

期刊介绍： The Journal of Nutrition (JN/J Nutr) publishes peer-reviewed original research papers covering all aspects of experimental nutrition in humans and other animal species; special articles such as reviews and biographies of prominent nutrition scientists; and issues, opinions, and commentaries on controversial issues in nutrition. Supplements are frequently published to provide extended discussion of topics of special interest.