利用新型眼部示踪剂鉴定青光眼患者体内视网膜淀粉样蛋白-Beta

IF 2 4区 医学 Q2 OPHTHALMOLOGY
Julie Pilotte, Sami Khoury, Ali Tafreshi, Zachary T Mandel, Svasti V Sharma, Peter Vanderklish, Stella T Sarraf, Alfredo A Sadun, Robert N Weinreb, Alex S Huang
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引用次数: 0

摘要

目的:描述人类青光眼视网膜中淀粉样蛋白-β(Aβ)的存在特征,并测试使用新型荧光 Aβ 结合小分子(AMDX-2011)对视网膜 Aβ 的识别:方法:从眼库获取患有(n=4)和未患有(n=4)青光眼的死后人类眼睛。对视网膜进行解剖、平装片和固定。使用平片对 Aβ 进行免疫荧光,对 AMDX-2011 进行染色,并使用荧光显微镜获取图像:结果:荧光显微镜显示青光眼视网膜中存在与 AMDX-2011 染色共定位的 Aβ 信号。共标记点出现在视网膜的所有象限,包括视神经颞侧视网膜。这些点主要位于视网膜的内层。与对照组视网膜相比,青光眼视网膜在所有位置都有更多的共标记点(P = 0.002-0.02)。与对照组视网膜相比,青光眼视网膜上象限的共标记点也更大(P = 0.02):结论:在人类青光眼视网膜中检测到了Aβ,并绘制了其分布图。AMDX-2011对Aβ的鉴定可能有助于未来检测青光眼患者Aβ的诊断测试。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of Retinal Amyloid-Beta in Ex-Vivo Human Glaucoma Eyes Using a Novel Ocular Tracer.

Purpose: To characterize the presence of amyloid-beta (Aβ) in human glaucoma retina and to test identification of retinal Aβ using a novel fluorescent Aβ-binding small molecule (AMDX-2011).

Methods: Post-mortem human eyes with (n=4) and without (n=4) glaucoma were acquired from an eye bank. Retinas were dissected, flat-mounted, and fixed. Using the flat-mounts, immunofluorescence was performed against Aβ, AMDX-2011 staining was conducted, and images were acquired using fluorescence microscopy.

Results: Fluorescence microscopy demonstrated presence of Aβ signal that co-localized with AMDX-2011 staining in glaucoma retina. Co-labeled puncta appeared in all quadrants of the retina, including retina temporal to the optic nerve. The puncta were mainly located within the inner layers of the retina. Glaucoma retinas had more co-labeled puncta than control retinas in all locations (P = 0.002-0.02). Co-labeled puncta were also larger in the superior quadrant of glaucoma compared to control retinas (P = 0.02).

Conclusions: Aβ was detected in human glaucomatous retina, and its distribution was mapped. AMDX-2011 identification of Aβ may lead to future diagnostic tests aimed at detecting Aβ in glaucoma patients.

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来源期刊
Journal of Glaucoma
Journal of Glaucoma 医学-眼科学
CiteScore
4.20
自引率
10.00%
发文量
330
审稿时长
4-8 weeks
期刊介绍: The Journal of Glaucoma is a peer reviewed journal addressing the spectrum of issues affecting definition, diagnosis, and management of glaucoma and providing a forum for lively and stimulating discussion of clinical, scientific, and socioeconomic factors affecting care of glaucoma patients.
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