Dhiya Altememy, Maryam Haji Ghasem Kashani, Amirahmadi Fateme, Pegah Khosravian
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引用次数: 0
摘要
迷迭香叶提取物是一种著名的药用植物,可诱导干细胞中神经营养素基因的表达和增殖。人脂肪源性干细胞(hASCs)具有高增殖和分化能力,很容易获得,而且提取时损伤最小。本研究评估了迷迭香提取物(RE)对hASCs诱导后48小时神经营养素基因表达的影响。用含有40%肉毒杆菌素的不同剂量的RE(30至70微克/毫升)处理48小时。与在含血清培养基中培养的细胞相比,用 40-60 微克/毫升的 RE 处理 48 小时的细胞中 NTF3、NTF4 和神经生长因子基因的表达,以及用 50-70 微克/毫升的 RE 处理 48 小时的细胞中 GDNF 的表达均有显著增加。然而,不同剂量的 RE 对处理细胞中脑源性神经营养因子基因的表达没有影响。与常规细胞培养相比,RE(50、60 µg/ml)可导致 hASCs 中神经营养因子基因表达的增加。因此,该方案可用于制备细胞疗法的理想细胞源。
New method to induce neurotrophin gene expression in human adipose-derived stem cells in vitro.
Rosemary leaf extract, a well-known medicinal plant, can induce neurotrophin gene expression and proliferation in stem cells. Human adipose-derived stem cells (hASCs) with high proliferation and differentiation capacity are easily accessible and can be extracted with the least damage. This study evaluated the effect of rosemary extract (RE) on neurotrophin gene expression at 48 h postinduction in hASCs. hASCs were isolated from healthy female donors, aged 28-35 years, who had undergone abdominal liposuction. Passage-4 stem cells were cultured and treated with different doses of RE (from 30 to 70 µg/ml) containing 40% carnosic acid for 48 h. Reverse transcription-polymerase chain reaction was used to check the expression of neurotrophin genes. The expression of NTF3, NTF4, and nerve growth factor genes in cells treated with 40-60 µg/ml and the expression of GDNF in cells treated with 50-70 µg/ml of RE for 48 h showed a significant increase compared to cells cultured in serum-containing medium. However, different doses of RE showed no effect on brain-derived neurotrophic factor gene expression in the treated cells. RE (50, 60 µg/ml) leads to an increase of neurotrophin gene expression in hASCs as compared to routine cell culture. Hence, this protocol can be used to prepare ideal cell sources for cell therapy.
期刊介绍:
Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is an Official Publication of Society of Pharmaceutical Education & Research™. It is an international journal published Quarterly. Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is available in online and print version. It is a peer reviewed journal aiming to communicate high quality original research work, reviews, short communications, case report, Ethics Forum, Education Forum and Letter to editor that contribute significantly to further the scientific knowledge related to the field of Pharmacy i.e. Pharmaceutics, Pharmacology, Pharmacognosy, Pharmaceutical Chemistry. Articles with timely interest and newer research concepts will be given more preference.