辣木灌溉液对变异链球菌细胞代谢变化的作用

IF 1.4 Q3 Pharmacology, Toxicology and Pharmaceutics
Cut Soraya, Fitri Yunita Batubara, Saskia L Nasroen, Subhaini Jakfar, Basri A Gani
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引用次数: 0

摘要

龋齿的主要病原体是变异链球菌(S. mutans)。Moringa oleifera(M. oleifera)具有抗氧化和抗菌特性,可通过应对氧化应激发挥作用,从而影响细菌细胞的新陈代谢。本研究考察了油橄榄对突变桿菌生长、毒性、葡聚糖结合蛋白(GBP)表达和核酸结构的影响。研究方法包括用分光光度法分析生长情况、用酶联免疫吸附法测定 GBP 定量、用(3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四唑溴化物)MTT 法测定细胞毒性、用傅立叶变换红外线法测定核酸变化以及用对接模拟法测定配体与受体的亲和力。结果表明,在 24 小时和 48 小时内,油橄榄在所有浓度下都能显著抑制 S. mutans 的生长(光密度)。此外,浓度为 25% 和 3.125% 的油橄榄能明显改变 S. mutans 的核酸,并显示出与 GBP 基因受体的高结合亲和力。因此,M. oleifera 可以抑制 S. mutans 的生长和 GBP 的产生,导致核酸变形,并与 GBP 受体强结合,突出了其在预防龋齿方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of Moringa oleifera irrigation solution on the cell metabolism change of Streptococcus mutans.

The principal etiological agent responsible for dental caries is Streptococcus mutans (S. mutans). The Moringa oleifera (M. oleifera) possesses antioxidant and antibacterial properties that function through the response to oxidative stress, which affects bacterial cell metabolism. This research examined M. oleifera impact on S. mutans growth, toxicity, glucan-binding protein (GBP) expression, and nucleic acid structure. Methods included spectrophotometry for growth analysis, enzyme-linked immunosorbent assay for GBP quantification, the (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) MTT assay for cytotoxicity, Fourier transform infrared for nucleic acid changes, and docking simulation for ligand-receptor affinity. Results showed that M. oleifera significantly inhibited S. mutans growth at all concentrations over 24 and 48 h (optical density <0.1), comparable to <300 CFU/mL. At 72 h, 6.25% and 3.125% concentrations were most effective, with chlorhexidine also showing stability at these times. A 3.125% concentration of M. oleifera notably reduced GBP production to below 15% and caused cell toxicity. Furthermore, 25% and 3.125% concentrations significantly altered S. mutans nucleic acids, and M. oleifera showed high binding affinity to the GBP gene receptor. Thus, M. oleifera can inhibit S. mutans growth and GBP production, cause nucleic acid deformation, and strongly bind to the GBP receptor, highlighting its potential in dental caries prevention.

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来源期刊
CiteScore
2.00
自引率
7.10%
发文量
44
审稿时长
20 weeks
期刊介绍: Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is an Official Publication of Society of Pharmaceutical Education & Research™. It is an international journal published Quarterly. Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is available in online and print version. It is a peer reviewed journal aiming to communicate high quality original research work, reviews, short communications, case report, Ethics Forum, Education Forum and Letter to editor that contribute significantly to further the scientific knowledge related to the field of Pharmacy i.e. Pharmaceutics, Pharmacology, Pharmacognosy, Pharmaceutical Chemistry. Articles with timely interest and newer research concepts will be given more preference.
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