从约旦糖尿病足溃疡患者中分离出的金黄色葡萄球菌的抗生素敏感性和生物膜形成能力。

IF 1.3 Q4 MICROBIOLOGY
Dima Owais, Rania M Al-Groom, Tareq Nayef AlRamadneh, Laila Alsawalha, Mohd Sajjad Ahmad Khan, Omar H Yousef, Shereen Z Burjaq
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引用次数: 0

摘要

背景和目的:微生物生物膜的特点是浮游细胞不可逆转地附着在表面,通常与高抗菌药耐药性和伤口愈合恶化有关。本研究旨在确定糖尿病患者糖尿病足溃疡(DFU)中金黄色葡萄球菌的流行率,并调查这些分离物的抗生素敏感性模式。此外,还筛选分离出的金黄色葡萄球菌的生物膜形成能力:共收集了 112 份糖尿病足患者的非愈合伤口拭子,并在不同的培养基上进行培养,以鉴定 98 个分离菌株并确定其特征。对分离出的金黄色葡萄球菌进行了检测,以确定其对不同抗菌剂的抗生素敏感性。此外,还使用组织培养板法(TPC)评估了金黄色葡萄球菌分离物的生物膜生成能力。通过 RT-PCR 检测了 icaA 基因的表达水平:研究结果表明,这些非愈合伤口产生了阳性培养物,平均每个样本有 1.67 个微生物。分离菌株对奥沙西林的耐药性最高(95.2%),对利奈唑胺的耐药性最低(3.7%)。所有分离物都是生物膜生产者,且与 icaA 基因表达水平有显著关联:本研究表明,金黄色葡萄球菌分离株具有很强的生物膜生成能力,这与糖尿病患者伤口的慢性化有关。对慢性伤口中的生物膜形成者进行常规筛查和抗生素敏感性检测将有助于早期治疗和预防其他并发症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibiotic susceptibility and biofilm forming ability of Staphylococcus aureus isolated from Jordanian patients with diabetic foot ulcer.

Background and objectives: Microbial biofilm is characterized by the irreversible attachment of planktonic cells to a surface and is usually associated with high antimicrobial resistance with worsening the wound healing. The objective of the study was to determine the prevalence of Staphylococcus aureus in diabetic foot ulcers (DFUs) of diabetic patients and to investigate antibiotic susceptibility patterns of these isolates. In addition to screen biofilm forming ability of isolated S. aureus.

Materials and methods: A total of 112 non-healing wound swabs of diabetic foot patients were collected and cultured on different culture media to identify and characterize 98 isolates. The S. aureus isolates were examined for their antibiotic susceptibility to different antimicrobial agents. Furthermore, S. aureus isolates were evaluated for their biofilm production capability using the Tissue Culture Plate Method (TPC). The level of icaA gene expression was determined by RT-PCR.

Results: The results of this study showed that these non-healing wounds yield positive cultures, with an average of 1.67 organisms per sample. The isolates showed highest resistance against oxacillin (95.2%) and lowest resistance against linezolid (3.7%). All isolates were biofilm producers and a significant association with the icaA gene expression level was recorded.

Conclusion: This study showed that S. aureus isolates have a great ability to produce biofilms that are associated with the chronicity of wounds in diabetic patients. Routine screening for biofilm formers in chronic wounds and their antibiotic susceptibility testing will help in early treatment and prevent any other complications.

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来源期刊
CiteScore
2.40
自引率
7.10%
发文量
96
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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