肯尼亚海岸线部分红树林内生真菌分离物的分子特征和抗菌潜力。

IF 2.8 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2024-09-06 eCollection Date: 2024-01-01 DOI:10.1155/2024/1261721
Teresia Nyambura Wacira, Huxley Mae Makonde, Carren Moraa Bosire, Cromwell Mwiti Kibiti
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引用次数: 0

摘要

抗药性病原微生物的不断出现和再次出现对人类健康造成了威胁。科学家们一直在努力寻找新型生物活性化合物和药物来克服这些障碍。本研究旨在描述红树林内生真菌的特征并评估其抗菌活性。研究人员从都铎溪、密达溪和加济湾采集了滨海红树(Heritiera littoralis)、粘柄红树(Rhizophora mucronata)、体茎红树(Bruguiera gymnorrhiza)、滨海红树(Avicennia marina)和花叶红树(Xylocarpus granatum)。根据 ITS 基因区的分析,共对 30 个真菌分离物进行了分子鉴定。推断出的系统发生树中的分离物隶属于曲霉属。采用碟片扩散法对 76 个分离的真菌内生菌中 38.2%的乙酸乙酯和丁醇粗提取物以及 8 个菌丝体样品进行了抗菌活性筛选,这些样品分别针对金黄色葡萄球菌(ATCC 27853)、大肠杆菌(ATCC 25922)和铜绿假单胞菌(ATCC 25923)。A. marina 和 R. mucronata 真菌内生菌最多,抗菌活性最高。七种真菌肉汤提取物对受试微生物的抗菌活性高于阳性对照。分离物的最低抑菌浓度(MIC)活性表明,芒柄蜡树根内生真菌分离物(RC6)的乙酸乙酯提取物(3.31 ± 0.01)是一种强抑制剂,因为它对铜绿假单胞菌的最低抑菌浓度(MIC)活性明显低于阳性对照(3.84 ± 0.00)(P < 0.05)。因此,这项研究证实,红树林物种蕴藏着具有抗菌活性的真菌分离物,因此可作为一种新型抗生素来源。建议从这些提取物中分离出纯化合物,进行进一步的生物活性测试和结构阐明,以考虑作为药物发现的先导分子。此外,还可以对这些分离物中生物活性增强的基因进行表征和生物工程改造,以用于制药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Characterization and Antibacterial Potential of Endophytic Fungal Isolates from Selected Mangroves along the Coastline of Kenya.

The increasing emergence and re-emergence of resistant pathogenic microbes causes a health threat to the human population. Scientists have been striving to find novel bioactive compounds and drugs to overcome these obstacles. This study aimed to characterize mangrove endophytic fungi and evaluate their antibacterial activity. Heritiera littoralis, Rhizophora mucronata, Bruguiera gymnorrhiza, Avicennia marina, and Xylocarpus granatum species were collected from Tudor Creek, Mida Creek, and Gazi Bay. A total of 30 fungal isolates were subjected to molecular identification based on analysis of their ITS gene region. The isolates in the inferred phylogenetic trees were affiliated with the genus Aspergillus. Ethyl acetate and butanol crude extracts of 38.2% of the 76 isolated fungal endophytes and eight mycelia samples were screened for antibacterial activity against Staphylococcus aureus (ATCC 27853), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 25923) using the disc diffusion method. A. marina and R. mucronata harbored the most fungal endophytes that showed the highest antibacterial activity. Seven fungal broth extracts exhibited higher antibacterial activities against the tested microorganisms than the positive control. The minimum inhibitory concentration (MIC) activity for the isolates demonstrated that the ethyl acetate extract of a root endophytic fungal isolate (RC6) (3.31 ± 0.01) of A. marina is a strong inhibitor since it showed significantly lower MIC activity compared to the positive control (3.84 ± 0.00) against Pseudomonas aeruginosa (P < 0.05). Therefore, this study confirms that mangrove species harbor fungal isolates that have antibacterial activity and hence could serve as a novel source of antibiotics. It is recommended that the pure compounds from these extracts be isolated for further bioactivity tests and structural elucidation for consideration as lead molecules in drug discovery. In addition, the genes responsible for the enhanced bioactivity in these isolates can be characterized and bioengineered for pharmaceutical application.

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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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