在黑暗中觅食:条斑跳甲地下幼虫嗅觉对十字花科挥发性物质的高度选择性调节

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yong Xiao, Chunmei Lei, Xue Wang, Raufa Batool, Fei Yin, Zhengke Peng, Xiangfeng Jing, Zhenyu Li
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引用次数: 0

摘要

地面昆虫的嗅觉系统是描述得最清楚的感知结构之一。然而,人们对地下昆虫如何在黑暗中导航觅食却知之甚少。在这里,我们研究了条纹蚤甲虫(Phyllotreta striolata Fabricius,鞘翅目:蝶形目)地下幼虫对寄主植物的偏好、嗅觉感受器以及嗅觉蛋白的特征。这种鞘翅目害虫的成虫和幼虫分别在地上和地下对十字花科作物造成严重危害。为了阐明嗅觉系统在地下幼虫寄主定位中的作用,我们首先通过二选一行为生物测定证明了芸苔科幼虫对芸苔属植物的明显偏爱。随后,通过扫描电子显微镜观察 SFB 幼虫头部的感觉器,发现与成虫相比,幼虫的嗅觉感觉器数量显著减少。然而,基本嗅觉感受器(sensilla basiconica)等重要的嗅觉感受器强调了幼虫嗅觉系统的不可或缺性。我们从先前的转录组数据中选择了四个幼虫特异性气味结合蛋白进行功能验证。功能研究发现,PstrOBP23 与 24 种十字花科植物的挥发性物质(包括 7 种异硫氰酸盐化合物)具有很强的结合亲和力。这表明 PstrOBP23 在幼虫的地下觅食行为中起着关键作用。此外,两种结合能力强的配体对 SFB 幼虫表现出明显的吸引或排斥活性。我们的研究结果为了解SFB地下幼虫的嗅觉系统提供了一个重要的视角,突出了幼虫特异性OBP对寄主植物挥发性物质的高度选择性调节。这些结果为制定有效的虫害防治策略提供了潜在的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Foraging in the darkness: Highly selective tuning of below-ground larval olfaction to Brassicaceae volatiles in striped flea beetle.

The olfactory system of above-ground insects is among the best described perceptual architectures. However, remarkably little is known about how below-ground insects navigate in the dark for foraging. Here, we investigated host plant preferences, olfactory sensilla and characterise olfactory proteins in below-ground larvae of the striped flea beetle (SFB) Phyllotreta striolata Fabricius (Coleoptera: Chrysomelidae). Both the adults and larvae of this coleopteran pest cause serious damage to Brassicaceous crops above and below ground, respectively. To elucidate the role of olfactory system in host location of below-ground larvae, we initially demonstrated that SFB larvae distinctly favoured Brassicaceae over other plant families by two-choice behavioural bioassay. Subsequently, scanning electron microscopy of sensilla in SFB larval head showed a significant reduction in the number of olfactory sensilla in larvae compared with adults. However, essential olfactory sensilla such as sensilla basiconica are underscoring the indispensability of the larval olfactory system. We selected four larval-specific odorant binding proteins for functional validation from our previous transcriptome data. Functional studies revealed that PstrOBP23 exhibits robust binding affinity to 24 volatiles of Brassicaceae plants, including seven isothiocyanate compounds. This suggests a pivotal role of PstrOBP23 in the foraging behaviour of the larvae below the ground. Moreover, two ligands displaying strong binding capacity exhibit apparent attractive or repellent activity towards SFB larvae. Our findings provide a crucial insight into the olfactory system of below-ground larvae in SFB, highlighting the highly selective tuning of larvae specific OBP to host plant volatiles. These results offer potential avenues for developing effective pest control strategies against SFB.

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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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