Savitha B Naik, N Kiran Kumar, P Annie Swathisha, Biji Brigit, H S Preetham, Chella S Karumaran
{"title":"穿心莲草本提取物对人牙髓干细胞细胞毒性、增殖和成骨/成牙分化的影响：体外研究。","authors":"Savitha B Naik, N Kiran Kumar, P Annie Swathisha, Biji Brigit, H S Preetham, Chella S Karumaran","doi":"10.4103/ijdr.ijdr_907_22","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Research has been conducted to assess the regenerative potential of dental pulp stem cells (DPSCs) following pretreatment of stem cells with certain molecules, bioactive compounds, plant extract and physical stimulation. Andrographis paniculata (AP) herbal extract with important medicinal properties is proven to have a preosteogenic effect on osteoblasts.</p><p><strong>Aim: </strong>This study aimed to determine the effect of various concentrations of AP extract on the cytotoxicity and osteogenic and odontogenic potential of DPSCs.</p><p><strong>Methods and material: </strong>Dental pulp stem cells were subjected to treatment with various concentrations of AP herbal extract (7 ug/ml, 5.2 ug/ml, 3.5 ug/ml, 1.7 ug/ml and 0.8 ug/ml), following which the cells were subjected to tests-3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) analysis for cytotoxicity and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) test for expression of genes (bone morphogenetic protein (BMP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP) and alkaline phosphatase (ALP)).</p><p><strong>Results: </strong>AP extract at concentration of 0.8 ug/ml-5.2 ug/ml had no cytotoxicity supporting cell growth. 3.5 ug/ml showed significant upregulation of genes on the third day.</p><p><strong>Conclusion: </strong>AP, a commonly occurring medicinal plant through its effect on DPSCs, could serve as an effective pretreatment modality for cell-based regenerative therapy and vital pulp therapy.</p>","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":" ","pages":"196-200"},"PeriodicalIF":0.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Andrographis paniculata Herbal Extract on Cytotoxicity, Proliferation and Osteogenic/Odontogenic Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study.\",\"authors\":\"Savitha B Naik, N Kiran Kumar, P Annie Swathisha, Biji Brigit, H S Preetham, Chella S Karumaran\",\"doi\":\"10.4103/ijdr.ijdr_907_22\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Research has been conducted to assess the regenerative potential of dental pulp stem cells (DPSCs) following pretreatment of stem cells with certain molecules, bioactive compounds, plant extract and physical stimulation. Andrographis paniculata (AP) herbal extract with important medicinal properties is proven to have a preosteogenic effect on osteoblasts.</p><p><strong>Aim: </strong>This study aimed to determine the effect of various concentrations of AP extract on the cytotoxicity and osteogenic and odontogenic potential of DPSCs.</p><p><strong>Methods and material: </strong>Dental pulp stem cells were subjected to treatment with various concentrations of AP herbal extract (7 ug/ml, 5.2 ug/ml, 3.5 ug/ml, 1.7 ug/ml and 0.8 ug/ml), following which the cells were subjected to tests-3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) analysis for cytotoxicity and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) test for expression of genes (bone morphogenetic protein (BMP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP) and alkaline phosphatase (ALP)).</p><p><strong>Results: </strong>AP extract at concentration of 0.8 ug/ml-5.2 ug/ml had no cytotoxicity supporting cell growth. 3.5 ug/ml showed significant upregulation of genes on the third day.</p><p><strong>Conclusion: </strong>AP, a commonly occurring medicinal plant through its effect on DPSCs, could serve as an effective pretreatment modality for cell-based regenerative therapy and vital pulp therapy.</p>\",\"PeriodicalId\":13311,\"journal\":{\"name\":\"Indian Journal of Dental Research\",\"volume\":\" \",\"pages\":\"196-200\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Dental Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/ijdr.ijdr_907_22\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/9/14 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"Dentistry\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Dental Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/ijdr.ijdr_907_22","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/14 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Dentistry","Score":null,"Total":0}
引用次数: 0
摘要
背景：已有研究评估了牙髓干细胞（DPSCs）在使用某些分子、生物活性化合物、植物提取物和物理刺激对干细胞进行预处理后的再生潜力。目的：本研究旨在确定不同浓度的穿心莲（AP）提取物对牙髓干细胞的细胞毒性、成骨和成牙潜能的影响：将牙髓干细胞置于不同浓度的 AP 草药提取物（7 ug/ml、5.2 ug/ml、3.5 ug/ml、1.7 ug/ml 和 0.8微克/毫升），然后对细胞进行3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑（MTT）细胞毒性分析和实时逆转录聚合酶链反应（RT-PCR）基因（骨形态发生蛋白（BMP）、骨钙素（OCN）、牙本质糖磷蛋白（DSPP）和碱性磷酸酶（ALP））表达检测：浓度为 0.8 微克/毫升-5.2 微克/毫升的 AP 提取物对细胞生长无细胞毒性。结论：AP 是一种常见的药用植物：AP是一种常见的药用植物，通过其对DPSCs的影响，AP可作为一种有效的预处理方式，用于基于细胞的再生疗法和牙髓治疗。
Effect of Andrographis paniculata Herbal Extract on Cytotoxicity, Proliferation and Osteogenic/Odontogenic Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study.
Background: Research has been conducted to assess the regenerative potential of dental pulp stem cells (DPSCs) following pretreatment of stem cells with certain molecules, bioactive compounds, plant extract and physical stimulation. Andrographis paniculata (AP) herbal extract with important medicinal properties is proven to have a preosteogenic effect on osteoblasts.
Aim: This study aimed to determine the effect of various concentrations of AP extract on the cytotoxicity and osteogenic and odontogenic potential of DPSCs.
Methods and material: Dental pulp stem cells were subjected to treatment with various concentrations of AP herbal extract (7 ug/ml, 5.2 ug/ml, 3.5 ug/ml, 1.7 ug/ml and 0.8 ug/ml), following which the cells were subjected to tests-3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) analysis for cytotoxicity and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) test for expression of genes (bone morphogenetic protein (BMP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP) and alkaline phosphatase (ALP)).
Results: AP extract at concentration of 0.8 ug/ml-5.2 ug/ml had no cytotoxicity supporting cell growth. 3.5 ug/ml showed significant upregulation of genes on the third day.
Conclusion: AP, a commonly occurring medicinal plant through its effect on DPSCs, could serve as an effective pretreatment modality for cell-based regenerative therapy and vital pulp therapy.
期刊介绍：
Indian Journal of Dental Research (IJDR) is the official publication of the Indian Society for Dental Research (ISDR), India section of the International Association for Dental Research (IADR), published quarterly. IJDR publishes scientific papers on well designed and controlled original research involving orodental sciences. Papers may also include reports on unusual and interesting case presentations and invited review papers on significant topics.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
