金黄色葡萄球菌脂质因子调节黑色素瘤细胞的聚集和侵袭

IF 4 3区 医学 Q2 CELL BIOLOGY
Disease Models & Mechanisms Pub Date : 2024-09-01 Epub Date: 2024-09-16 DOI:10.1242/dmm.050770
Morgan A Giese, Gayathri Ramakrishnan, Laura H Steenberge, Jerome X Dovan, John-Demian Sauer, Anna Huttenlocher
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引用次数: 0

摘要

微生物群可影响癌症的发生和发展。然而,人们对皮肤微生物群在黑色素瘤中的作用知之甚少。在这里,我们利用斑马鱼黑色素瘤模型来探究金黄色葡萄球菌对黑色素瘤侵袭的影响。我们发现,金黄色葡萄球菌产生的因子能增强黑色素瘤在斑马鱼幼体中的侵袭和扩散。我们使用了一种已发表的体外三维集群形成试验,该试验将集群的增加与肿瘤的侵袭相关联。金黄色葡萄球菌上清液可增加黑色素瘤细胞的集群,Rho-激酶抑制剂可抑制集群,这表明 Rho-GTP 酶在其中发挥了作用。黑色素瘤集聚反应对金黄色葡萄球菌具有特异性,而对包括表皮葡萄球菌在内的其他葡萄球菌没有特异性。我们的研究结果表明,金黄色葡萄球菌通过脂肪酶 Sal2(正式名称为 GehB)产生的脂质促进黑色素瘤的聚集和侵袭。综上所述,这些研究结果表明,特定的细菌产物介导了斑马鱼黑色素瘤的侵袭性迁移。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Staphylococcus aureus lipid factors modulate melanoma cell clustering and invasion.

The microbiome can influence cancer development and progression. However, less is known about the role of the skin microbiota in melanoma. Here, we took advantage of a zebrafish melanoma model to probe the effects of Staphylococcus aureus on melanoma invasion. We found that S. aureus produces factors that enhance melanoma invasion and dissemination in zebrafish larvae. We used a published in vitro 3D cluster formation assay that correlates increased clustering with tumor invasion. S. aureus supernatant increased clustering of melanoma cells and was abrogated by a Rho-Kinase inhibitor, implicating a role for Rho-GTPases. The melanoma clustering response was specific to S. aureus but not to other staphylococcal species, including S. epidermidis. Our findings suggest that S. aureus promotes melanoma clustering and invasion via lipids generated by the lipase Sal2 (officially known as GehB). Taken together, these findings suggest that specific bacterial products mediate melanoma invasive migration in zebrafish.

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来源期刊
Disease Models & Mechanisms
Disease Models & Mechanisms 医学-病理学
CiteScore
6.60
自引率
7.00%
发文量
203
审稿时长
6-12 weeks
期刊介绍: Disease Models & Mechanisms (DMM) is an online Open Access journal focusing on the use of model systems to better understand, diagnose and treat human disease.
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