体外消化研究：比较预消化甘油分解产物和富含长链多不饱和脂肪酸的油类 (LCPUFA)，作为向早产新生儿提供 LCPUFA 的一种策略。

IF 2.9 Q3 NUTRITION & DIETETICS

Clinical nutrition ESPEN Pub Date : 2024-09-19 DOI:10.1016/j.clnesp.2024.09.011

Assamae Chabni , Blanca Pardo de Donlebún , Celia Bañares , Carlos F. Torres

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引用次数: 0

摘要

背景与目的：保持花生四烯酸（ARA）和二十二碳六烯酸（DHA）的充足供应对早产儿的最佳生长至关重要。本研究旨在通过体外消化模型评估和比较 ARA 和 DHA 油与其预消化产物的消化率和生物可及性：方法：体外胃肠道消化模型分为两个阶段：胃消化和肠道消化。将两种富含多不饱和脂肪酸的油（ARA 和 DHA 油）样本及其通过酶甘油分解产生的预消化产物（2:1，ARA: DHA）消化 120 分钟。最终得到的消化产物由三相组成：上层油相（OP）含有未消化的物种，中间胶束相（MP）含有消化的和生物可获取的脂类，沉淀相（PP）含有不溶性化合物。取等分样品监测反应，然后进行脂质提取和分析：结果：与甘油分解产物（GP）油相（13.9%）相比，根据油相的百分比（分别为 26.7% 和 20%）观察到 ARA 和 DHA 油的消化率较低。GP 中的胶束相最高（约 83%）。另一方面，在 ARA 和 DHA 油的消化产物（DP）中，单甘油酯（MAG）含量较低，分别为 4.3% 和 9.2%，而在 GP 的消化产物（DP）中，单甘油酯（MAG）含量为 15%：考虑到油相、胶束相和 MAG 含量的百分比，可以得出结论：GP 比其前体油更容易消化，ARA 和 DHA 更容易被生物体吸收。

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In vitro digestion study comparing a predigested glycerolysis product versus long-chain polyunsaturated fatty acid-rich oils (LCPUFA) as a strategy for administering LCPUFA to preterm neonates

Background & aims

Maintaining an adequate supply of arachidonic acid (ARA) and docosahexaenoic acid (DHA) is essential for optimal growth of preterm infants. This study aims to evaluate and compare the digestibility and bioaccessibility of ARA and DHA oils compared to their predigested product through an in vitro digestion model.

Methods

An in vitro gastrointestinal digestion model was used in two stages: gastric digestion and intestinal digestion. Samples of two polyunsaturated rich oils (ARA and DHA oils) and their predigested product (2:1, ARA: DHA) produced by enzymatic glycerolysis have been digested for 120 min. The final digestion product obtained was composed of three phases: an upper oily phase (OP) containing the undigested species, an intermediate micellar phase (MP) containing digested and bioaccessible lipids, and a precipitate phase (PP) with insoluble compounds. The reaction was monitored by taking aliquots and their subsequent lipid extraction and analysis.

Results

Poorer digestibility for ARA and DHA oils was observed based on the percentage of the oily phase (26.7% and 20%, respectively) found compared to the glycerolysis product (GP) oily phase (13.9%). The highest micellar phase was found in the GP (approx. 83%). On the other hand, the monoglyceride (MAG) content was lower in the digestion product (DP) from ARA and DHA oils, 4.3% and 9.2%, respectively, compared to the MAG observed in the DP of GP (15%).

Conclusion

Considering the percentage of oily phase, micellar phase, and the MAG content, it can be concluded that the GP is more digestible and ARA and DHA are more bioaccessible than in its precursor oils.

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来源期刊

Clinical nutrition ESPEN NUTRITION & DIETETICS-

CiteScore

4.90

自引率

3.30%

发文量

512

期刊介绍： Clinical Nutrition ESPEN is an electronic-only journal and is an official publication of the European Society for Clinical Nutrition and Metabolism (ESPEN). Nutrition and nutritional care have gained wide clinical and scientific interest during the past decades. The increasing knowledge of metabolic disturbances and nutritional assessment in chronic and acute diseases has stimulated rapid advances in design, development and clinical application of nutritional support. The aims of ESPEN are to encourage the rapid diffusion of knowledge and its application in the field of clinical nutrition and metabolism. Published bimonthly, Clinical Nutrition ESPEN focuses on publishing articles on the relationship between nutrition and disease in the setting of basic science and clinical practice. Clinical Nutrition ESPEN is available to all members of ESPEN and to all subscribers of Clinical Nutrition.