通过表型和蛋白质组评估解码蛋鸡的生育期。

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
M A Kabir, H Ruan, L Rong, M A Horaira, X Wu, L Wang, Y Wang, J Cai, S Han, S Li
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引用次数: 0

摘要

1.本研究确定了参与鸡长生育期(DF)的有效指标和蛋白质。 2. 利用7个表型性状指标对中国新华鸡(900只)的3个品系进行了比较,并根据重复性值确定了最佳品系。随后,进行了差异表达分析、功能注释和蛋白质相互作用(PPI)网络分析,以研究通路和枢纽蛋白。研究发现,受精卵数(FN)和最大受精卵数(MCF)是七项指标中重复性最高的。研究发现了 231 个差异表达蛋白,其中 144 个下调,87 个上调。差异表达的蛋白质在细胞的各个区室(包括细胞膜、细胞质和 GTP 结合)中表现出高度的聚集性。研究发现了多种通路,包括紧密连接和粘连连接、TGF-beta 信号传导、自噬-动物、肌动蛋白细胞骨架调控和核糖体,这些通路可能会调控该性状。三个枢纽蛋白 KRAS、RPL5(p p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Decoding the duration of fertility of laying chicken through phenotypic and proteomic evaluation.

1. This study determined the effective indicators and proteins involved in long-duration fertility (DF) in chickens.2. Three lines of Chinese Xinhua chickens (900) were compared using seven phenotypic trait indicators, and the best was determined based on repeatability value. Subsequently, differential expression analysis, functional annotation and protein-protein interaction (PPI) network analyses were performed to investigate the pathways and hub proteins. Finally, qPCR analysis was conducted to validate the expression of identified hub proteins, and functional annotation with previously published genes was performed to explain how hub proteins work to maintain the trait.3. The study found that the number of fertilised eggs (FN) and maximum fertilised eggs (MCF) were the most repeatable among the seven indicators. It identified 231 differentially expressed proteins, with 144 being down-regulated and 87 being up-regulated. The differentially expressed proteins exhibited high clustering within various cellular compartments, including the cytosol and cytoplasm and GTP binding. Multiple pathways were identified, including tight and adherens junctions, TGF-beta signalling, autophagy-animal, regulation of actin cytoskeleton and the ribosome that may regulate the trait. Three hub proteins, KRAS, RPL5 (p < 0.001), and HSPA4 (p < 0.01), were significantly differentially expressed between high and low DF groups.4. This study identified FN and MCF as effective indicators for addressing DF. As it is a quantitative trait, KRAS, HSPA4, and RPL5 are potential hub proteins that work with other genes to maintain the trait.

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来源期刊
British Poultry Science
British Poultry Science 农林科学-奶制品与动物科学
CiteScore
3.90
自引率
5.00%
发文量
88
审稿时长
4.5 months
期刊介绍: From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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