血浆外泌体 miR-203a-3p 是评估乳腺癌患者肿瘤进展的潜在液体活检标记物

IF 3.3 4区 医学 Q2 ONCOLOGY
Breast Cancer : Targets and Therapy Pub Date : 2024-09-18 eCollection Date: 2024-01-01 DOI:10.2147/BCTT.S478328
Xin Yang, Lei Fan, Jicheng Huang, Yongjun Li
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引用次数: 0

摘要

背景:及时检测乳腺癌(BC)患者的肿瘤进展对治疗管理和预后至关重要。血浆外泌体 miRNA 是监测肿瘤进展的潜在液体活检标记物,但它们在 BC 中的作用仍不清楚:在TCGA数据库中,我们首先通过比较癌旁组织、I期BC组织和II-III期BC组织(n = 1026)中的miRNA表达,筛选出与BC进展显著相关的miRNA。对候选 miRNA 进行了 Cox 回归分析和生存分析,以探讨其预后价值(n = 848)。KEGG、GO和PPI分析用于确定与癌症相关的富集通路。最后,通过对我们收集的 45 例 BC 患者(14 例 I 期,31 例 II-III 期)和 5 例健康对照的血浆外泌体 miRNA 进行测序和分析,结合 qRT-PCR 分析评估血浆外泌体和 BC 组织中候选基因表达的相关性,从而评估候选 miRNA 作为液体活检标志物的潜力:结果:我们发现,在TCGA数据集中,只有miR-203a-3p随着BC的进展而逐渐升高,并与不良预后相关。miR-203a-3p的潜在靶基因富集在与肿瘤进展相关的通路中,其中48个基因的下调与预后不良有关。更重要的是,研究还发现血浆外泌体 miR-203a-3p 会随着 BC 的进展而逐渐增加,其表达量与 BC 组织中的 miR-203a-3p 呈正相关。这一结果表明,血浆外泌体miR-203a-3p可能反映了肿瘤组织中miR-203a-3p的表达,可作为监测BC进展的潜在液体活检标志物:我们首次发现,miR-203a-3p 的升高与 BC 病程进展和预后不良有关。我们的研究结果表明,血浆外泌体miR-203a-3p有可能成为一种液体活检标志物,用于评估BC患者的病情进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Plasma Exosome miR-203a-3p is a Potential Liquid Biopsy Marker for Assessing Tumor Progression in Breast Cancer Patients.

Background: Timely detection of tumor progression in breast cancer (BC) patients is critical for therapeutic management and prognosis. Plasma exosomal miRNAs are potential liquid biopsy markers for monitoring tumor progression, but their roles in BC remain unclear.

Methods: In the TCGA database, we first screened for miRNAs significantly associated with BC progression by comparing miRNA expression in para-carcinoma tissues, stage I BC tissues, and stage II-III BC tissues (n = 1026). Cox regression analyses and survival analyses were performed on candidate miRNAs to explore their prognostic value (n = 848). KEGG, GO, and PPI analyses were used to identify enriched pathways associated with cancer. Finally, the potential of candidate miRNAs as liquid biopsy markers was evaluated by sequencing and analyzing plasma exosomal miRNAs from our collection of 45 BC patients (14 in stage I, 31 in stage II-III) and 5 healthy controls, combined with qRT-PCR analysis to assess the correlation of candidate gene expression in plasma exosomes and BC tissues.

Results: We found that only miR-203a-3p was progressively elevated with BC progression and was associated with poor prognosis in the TCGA dataset. Its potential target genes were enriched in pathways related to tumor progression, and the downregulation of 48 of these genes was associated with poor prognosis. More importantly, plasma exosomal miR-203a-3p was also found to gradually increase with BC progression, and its expression was positively correlated with miR-203a-3p in BC tissues. This result suggests that plasma exosomal miR-203a-3p may reflect the expression of miR-203a-3p in tumor tissues and serve as a potential liquid biopsy marker for monitoring BC progressions.

Conclusion: We found for the first time that elevated miR-203a-3p was associated with BC progression and poor prognosis. Our findings suggested that plasma exosomal miR-203a-3p could hold potential as a liquid biopsy marker for evaluating BC progression in patients.

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来源期刊
CiteScore
4.10
自引率
0.00%
发文量
40
审稿时长
16 weeks
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