重组 SARS-CoV-2 穗状病毒蛋白变体对红细胞参数和红细胞分布宽度的影响

IF 4.1 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal Pub Date : 2024-09-07 DOI:10.1016/j.bj.2024.100787

Francesco Dima, Gian Luca Salvagno, Giuseppe Lippi

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引用次数: 0

摘要

我们计划进行一系列实验，研究不同 SARS-CoV-2 变体的尖峰蛋白在影响红细胞生物学特性方面可能发挥的作用。在使用四种不同浓度的 SARS-CoV-2 重组尖峰蛋白的所有样本中，红细胞计数、血红蛋白和平均血红蛋白 (MHC) 的值均无变化。重组 SARS-CoV-2 Alpha 和 Delta 加标蛋白的最终浓度为 2 和 20 纳克/毫升时，平均血球容积（MCV）均有明显增加。在使用 20 纳克/毫升所有 SARS-CoV-2 重组尖峰蛋白处理的样本中，红细胞分布宽度（RDW）值显著增加，在使用 Omicron 重组尖峰蛋白处理的样本中达到最高值。在添加了重组 SARS-CoV-2 加穗蛋白的样本中，尤其是在α和δ变体样本中，血涂片检查结果显示有血凝和胭脂虫现象。

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Effects of recombinant SARS-CoV-2 spike protein variants on red blood cells parameters and red blood cell distribution width

We planned a series of experiments to investigate the possible role of spike protein of different SARS-CoV-2 variants in influencing erythrocyte biology. The values of erythrocyte count, hemoglobin, and mean corpuscular hemoglobin (MHC) did not vary across all samples challenged with both concentrations of the four different SARS-CoV-2 recombinant spike proteins. A significant increase in mean corpuscular volume (MCV) was observed with the recombinant SARS-CoV-2 Alpha and Delta spike proteins at both 2 and 20 ng/mL final concentrations. Red blood cell distribution width (RDW) values increased significantly in samples treated with 20 ng/mL of all SARS-CoV-2 recombinant spike proteins and reached the highest values in samples treated with Omicron recombinant spike protein. Blood smear revision evidenced hemagglutination and rouleaux in samples to which recombinant SARS-CoV-2 spike proteins were added, especially in those with Alpha and Delta variants.

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来源期刊

Biomedical Journal Medicine-General Medicine

CiteScore

11.60

自引率

1.80%

发文量

128

审稿时长

42 days

期刊介绍： Biomedical Journal publishes 6 peer-reviewed issues per year in all fields of clinical and biomedical sciences for an internationally diverse authorship. Unlike most open access journals, which are free to readers but not authors, Biomedical Journal does not charge for subscription, submission, processing or publication of manuscripts, nor for color reproduction of photographs. Clinical studies, accounts of clinical trials, biomarker studies, and characterization of human pathogens are within the scope of the journal, as well as basic studies in model species such as Escherichia coli, Caenorhabditis elegans, Drosophila melanogaster, and Mus musculus revealing the function of molecules, cells, and tissues relevant for human health. However, articles on other species can be published if they contribute to our understanding of basic mechanisms of biology. A highly-cited international editorial board assures timely publication of manuscripts. Reviews on recent progress in biomedical sciences are commissioned by the editors.