利用 DCE-MRI 绘制小鼠脑肿瘤间质流体动力学图的新方法。

IF 4.2 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Methods Pub Date : 2024-09-14 DOI:10.1016/j.ymeth.2024.09.008

Cora Carman-Esparza , Kathryn Kingsmore , Andrea Vaccari , Skylar Davis , Jessica Cunningham , Maosen Wang , Jennifer Munson

{"title":"利用 DCE-MRI 绘制小鼠脑肿瘤间质流体动力学图的新方法。","authors":"Cora Carman-Esparza , Kathryn Kingsmore , Andrea Vaccari , Skylar Davis , Jessica Cunningham , Maosen Wang , Jennifer Munson","doi":"10.1016/j.ymeth.2024.09.008","DOIUrl":null,"url":null,"abstract":"<div><div>We present a comprehensive methodology for measuring heterogeneous interstitial fluid flow in murine brain tumors using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) coupled with the computational tool, <em>Lymph4D</em>. This four-part protocol encompasses glioma cell preparation, tumor inoculation, MRI imaging protocol, and histological verification using Evans Blue. While conventional DCE-MRI analysis primarily focuses on vascular perfusion, our methods reveal untapped potential to extract crucial information about interstitial fluid dynamics, including directions, velocities, and diffusion coefficients. This methodology extends beyond glioma research, with applicability to conditions routinely imaged with DCE-MRI, thereby offering a versatile tool for investigating interstitial fluid dynamics across a wide range of diseases and conditions. Our methodology holds promise for accelerating discoveries and advancements in biomedical research, ultimately enhancing diagnostic and therapeutic strategies for a wide range of diseases and conditions.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"231 ","pages":"Pages 78-93"},"PeriodicalIF":4.2000,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A novel methodology for mapping interstitial fluid dynamics in murine brain tumors using DCE-MRI\",\"authors\":\"Cora Carman-Esparza , Kathryn Kingsmore , Andrea Vaccari , Skylar Davis , Jessica Cunningham , Maosen Wang , Jennifer Munson\",\"doi\":\"10.1016/j.ymeth.2024.09.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>We present a comprehensive methodology for measuring heterogeneous interstitial fluid flow in murine brain tumors using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) coupled with the computational tool, <em>Lymph4D</em>. This four-part protocol encompasses glioma cell preparation, tumor inoculation, MRI imaging protocol, and histological verification using Evans Blue. While conventional DCE-MRI analysis primarily focuses on vascular perfusion, our methods reveal untapped potential to extract crucial information about interstitial fluid dynamics, including directions, velocities, and diffusion coefficients. This methodology extends beyond glioma research, with applicability to conditions routinely imaged with DCE-MRI, thereby offering a versatile tool for investigating interstitial fluid dynamics across a wide range of diseases and conditions. Our methodology holds promise for accelerating discoveries and advancements in biomedical research, ultimately enhancing diagnostic and therapeutic strategies for a wide range of diseases and conditions.</div></div>\",\"PeriodicalId\":390,\"journal\":{\"name\":\"Methods\",\"volume\":\"231 \",\"pages\":\"Pages 78-93\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2024-09-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Methods\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1046202324002068\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1046202324002068","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

摘要

我们介绍了一种利用动态对比增强磁共振成像（DCE-MRI）和计算工具 Lymph4D 测量小鼠脑肿瘤异质性间质流的综合方法。该方案由四个部分组成，包括胶质瘤细胞制备、肿瘤接种、磁共振成像方案和使用伊文思蓝进行组织学验证。传统的 DCE-MRI 分析主要关注血管灌注，而我们的方法揭示了提取间质流体动力学关键信息（包括方向、速度和扩散系数）的未开发潜力。这种方法不仅适用于胶质瘤研究，还适用于 DCE-MRI 常规成像的病症，从而为研究各种疾病和病症的间质流体动力学提供了多功能工具。我们的方法有望加速生物医学研究的发现和进步，最终提高各种疾病和病症的诊断和治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

A novel methodology for mapping interstitial fluid dynamics in murine brain tumors using DCE-MRI

We present a comprehensive methodology for measuring heterogeneous interstitial fluid flow in murine brain tumors using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) coupled with the computational tool, Lymph4D. This four-part protocol encompasses glioma cell preparation, tumor inoculation, MRI imaging protocol, and histological verification using Evans Blue. While conventional DCE-MRI analysis primarily focuses on vascular perfusion, our methods reveal untapped potential to extract crucial information about interstitial fluid dynamics, including directions, velocities, and diffusion coefficients. This methodology extends beyond glioma research, with applicability to conditions routinely imaged with DCE-MRI, thereby offering a versatile tool for investigating interstitial fluid dynamics across a wide range of diseases and conditions. Our methodology holds promise for accelerating discoveries and advancements in biomedical research, ultimately enhancing diagnostic and therapeutic strategies for a wide range of diseases and conditions.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Methods 生物-生化研究方法

CiteScore

9.80

自引率

2.10%

发文量

222

审稿时长

11.3 weeks

期刊介绍： Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.