戊四唑诱导的癫痫发作会导致幼年雄性 Wistar 大鼠海马和颞皮层小胶质细胞和星形胶质细胞表型的短期改变

IF 2.9 3区 医学 Q2 NEUROSCIENCES
Maria V. Zakharova, Anna A. Kovalenko, Olga E. Zubareva, Alexander P. Schwarz, Tatiana Y. Postnikova, Alina M. Trofimova, Aleksey V. Zaitsev
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引用次数: 0

摘要

在各种病理过程中,星形胶质细胞和小胶质细胞可采用两种不同的表型:神经毒性 A1/M1 和神经保护性 A2/M2。最近的证据表明,这些细胞在癫痫发生过程中扮演着重要角色。本研究旨在描述戊四唑诱导癫痫发作后 3 h、1、3 和 7 天,年轻雄性 Wistar 大鼠海马和颞叶皮层中星形胶质细胞和小胶质细胞的表型。采用 RT-qPCR 检测神经胶质基因(Gfap、Aif1、Slc1a1、Slc1a2、Slc1a3、Itpr2、Gdnf、Bdnf、Fgf2、Tgfb、Il1b、Tnf、Il1rn、Lcn2、S100a10、Nlrp3、Arg1)的表达。在颞叶皮层癫痫发作后的第一天,神经胶质基因的表达发生了最显著的变化。观察到 Gfap、Slc1a2、Slc1a1、Il1b、Tnfa、Bdnf 和 Fgf2 基因以及 A2 星形胶质细胞条件标记 S100a10 的表达增加。在癫痫发作后的第一天,在海马中观察到 Gfap 和 Slc1a2 基因的表达增加。然而,与皮层中观察到的变化相反,海马中的 Il1rn、Bdnf、Tgfb 和 Arg1 基因的变化却与之相反。然而,Western 印迹分析并未证实 GFAP 和 EAAT2 蛋白水平的变化。相反,更全面的免疫组化分析证实,癫痫发作 1 天后,海马中 GFAP 阳性细胞的数量有所增加。根据以上证据,我们可以得出结论:3 周大的大鼠发生一次惊厥发作后,星形胶质细胞会被短暂激活并极化为神经保护表型(A2)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Pentylenetetrazole-Induced Seizures Cause Short-Term Changes in the Phenotype of Microglial and Astroglial Cells in the Hippocampus and Temporal Cortex of Young Male Wistar Rats

Pentylenetetrazole-Induced Seizures Cause Short-Term Changes in the Phenotype of Microglial and Astroglial Cells in the Hippocampus and Temporal Cortex of Young Male Wistar Rats

Astrocytes and microglia can adopt two distinct phenotypes in various pathological processes: neurotoxic A1/M1 and neuroprotective A2/M2. Recent evidence suggests that these cells play a significant role in epileptogenesis. The objective of this study was to characterize the phenotype of astrocytes and microglial cells in the hippocampus and temporal cortex of young male Wistar rats at 3 h, 1, 3, and 7 days after pentylenetetrazole-induced seizures. RT-qPCR was employed to examine the expression of glial genes (Gfap, Aif1, Slc1a1, Slc1a2, Slc1a3, Itpr2, Gdnf, Bdnf, Fgf2, Tgfb, Il1b, Tnf, Il1rn, Lcn2, S100a10, Nlrp3, Arg1). The most notable alterations in the expression of glial genes were observed on the first day following seizures in the temporal cortex. An increase in the expression of the Gfap, Slc1a2, Slc1a1, Il1b, Tnfa, Bdnf, and Fgf2 genes, and the A2 astrocyte condition marker S100a10, was observed. An increase in the expression of the Gfap and Slc1a2 genes was observed in the hippocampus on the first day after seizures. However, in contrast to the changes observed in the cortex, the changes in the hippocampus were opposite for the Il1rn, Bdnf, Tgfb, and Arg1 genes. Nevertheless, the alterations in GFAP and EAAT2 protein levels were not corroborated by Western blot analysis. Conversely, a more comprehensive immunohistochemical analysis confirmed an augmentation in the number of GFAP-positive cells in the hippocampus 1 day after seizures. Based on the presented evidence, we can conclude that a single convulsive seizure episode in 3-week-old rats results in transient astroglial activation and polarization to a neuroprotective phenotype (A2).

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来源期刊
Journal of Neuroscience Research
Journal of Neuroscience Research 医学-神经科学
CiteScore
9.50
自引率
2.40%
发文量
145
审稿时长
1 months
期刊介绍: The Journal of Neuroscience Research (JNR) publishes novel research results that will advance our understanding of the development, function and pathophysiology of the nervous system, using molecular, cellular, systems, and translational approaches. JNR covers both basic research and clinical aspects of neurology, neuropathology, psychiatry or psychology. The journal focuses on uncovering the intricacies of brain structure and function. Research published in JNR covers all species from invertebrates to humans, and the reports inform the readers about the function and organization of the nervous system, with emphasis on how disease modifies the function and organization.
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