{"title":"代谢组学揭示 AgNPs 在环境相关浓度下对大型蚤的持久毒性机制","authors":"Qianqian Xiang, Qin Qin Li, Peng Wang, Hao-Cheng Yang, Zi-Hao Fu, Xiang Liang, Li Qiang Chen","doi":"10.1039/d4en00350k","DOIUrl":null,"url":null,"abstract":"Although the ecotoxicity of silver nanoparticles (AgNPs) has been of great concern, the persistence and underlying mechanisms of AgNPs toxicity remain understudied. This study explored the persistent mechanisms of AgNPs toxicity at two sizes (AgNP-10 nm and AgNP-70 nm at 2 μg/L) to Daphnia magna using traditional toxicological methods alongside metabolomics analyses during exposure and recovery phases. After 24 h, both AgNP-10 and -70 nm exposures resulted in high silver accumulation levels in D. magna, leading to reduced heart rate and paddling frequency. Despite a significant decrease in silver content after 24 h of recovery, the heart rate reduction persisted in AgNP-exposed D. magna. Metabolomics analysis revealed differential expression of 53 and 54 metabolites induced by AgNP-10 and -70 nm exposures, respectively, primarily enriched in lipid metabolism pathways. Following the recovery period, AgNP-10 and -70 nm induced differential expression of 71 and 110 metabolites, respectively, mainly enriched in lipid metabolism and protein digestion and uptake pathways. These findings indicate that the persistence of toxicity of D. magna induced by AgNPs at physiological and metabolomic levels, predominantly attributed to silver retention and damage to D. magna’s digestive system. Overall, this study provides novel insights into the mechanism underlying the persistence of AgNPs toxicity to aquatic organism.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":null,"pages":null},"PeriodicalIF":5.8000,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Metabolomics reveals the mechanism of persistent toxicity of AgNPs at environmentally relevant concentrations to Daphnia magna\",\"authors\":\"Qianqian Xiang, Qin Qin Li, Peng Wang, Hao-Cheng Yang, Zi-Hao Fu, Xiang Liang, Li Qiang Chen\",\"doi\":\"10.1039/d4en00350k\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Although the ecotoxicity of silver nanoparticles (AgNPs) has been of great concern, the persistence and underlying mechanisms of AgNPs toxicity remain understudied. This study explored the persistent mechanisms of AgNPs toxicity at two sizes (AgNP-10 nm and AgNP-70 nm at 2 μg/L) to Daphnia magna using traditional toxicological methods alongside metabolomics analyses during exposure and recovery phases. After 24 h, both AgNP-10 and -70 nm exposures resulted in high silver accumulation levels in D. magna, leading to reduced heart rate and paddling frequency. Despite a significant decrease in silver content after 24 h of recovery, the heart rate reduction persisted in AgNP-exposed D. magna. Metabolomics analysis revealed differential expression of 53 and 54 metabolites induced by AgNP-10 and -70 nm exposures, respectively, primarily enriched in lipid metabolism pathways. Following the recovery period, AgNP-10 and -70 nm induced differential expression of 71 and 110 metabolites, respectively, mainly enriched in lipid metabolism and protein digestion and uptake pathways. These findings indicate that the persistence of toxicity of D. magna induced by AgNPs at physiological and metabolomic levels, predominantly attributed to silver retention and damage to D. magna’s digestive system. Overall, this study provides novel insights into the mechanism underlying the persistence of AgNPs toxicity to aquatic organism.\",\"PeriodicalId\":73,\"journal\":{\"name\":\"Environmental Science: Nano\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.8000,\"publicationDate\":\"2024-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Science: Nano\",\"FirstCategoryId\":\"6\",\"ListUrlMain\":\"https://doi.org/10.1039/d4en00350k\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Science: Nano","FirstCategoryId":"6","ListUrlMain":"https://doi.org/10.1039/d4en00350k","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Metabolomics reveals the mechanism of persistent toxicity of AgNPs at environmentally relevant concentrations to Daphnia magna
Although the ecotoxicity of silver nanoparticles (AgNPs) has been of great concern, the persistence and underlying mechanisms of AgNPs toxicity remain understudied. This study explored the persistent mechanisms of AgNPs toxicity at two sizes (AgNP-10 nm and AgNP-70 nm at 2 μg/L) to Daphnia magna using traditional toxicological methods alongside metabolomics analyses during exposure and recovery phases. After 24 h, both AgNP-10 and -70 nm exposures resulted in high silver accumulation levels in D. magna, leading to reduced heart rate and paddling frequency. Despite a significant decrease in silver content after 24 h of recovery, the heart rate reduction persisted in AgNP-exposed D. magna. Metabolomics analysis revealed differential expression of 53 and 54 metabolites induced by AgNP-10 and -70 nm exposures, respectively, primarily enriched in lipid metabolism pathways. Following the recovery period, AgNP-10 and -70 nm induced differential expression of 71 and 110 metabolites, respectively, mainly enriched in lipid metabolism and protein digestion and uptake pathways. These findings indicate that the persistence of toxicity of D. magna induced by AgNPs at physiological and metabolomic levels, predominantly attributed to silver retention and damage to D. magna’s digestive system. Overall, this study provides novel insights into the mechanism underlying the persistence of AgNPs toxicity to aquatic organism.
期刊介绍:
Environmental Science: Nano serves as a comprehensive and high-impact peer-reviewed source of information on the design and demonstration of engineered nanomaterials for environment-based applications. It also covers the interactions between engineered, natural, and incidental nanomaterials with biological and environmental systems. This scope includes, but is not limited to, the following topic areas:
Novel nanomaterial-based applications for water, air, soil, food, and energy sustainability
Nanomaterial interactions with biological systems and nanotoxicology
Environmental fate, reactivity, and transformations of nanoscale materials
Nanoscale processes in the environment
Sustainable nanotechnology including rational nanomaterial design, life cycle assessment, risk/benefit analysis