Telonemia 原生动物的全球淡水分布。

Roudaina Boukheloua,Indranil Mukherjee,Hongjae Park,Karel Šimek,Vojtěch Kasalický,Maxon Ngochera,Hans-Peter Grossart,Antonio Picazo-Mozo,Antonio Camacho,Pedro J Cabello-Yeves,Francisco Rodriguez-Valera,Cristiana Callieri,Adrian-Stefan Andrei,Jakob Pernthaler,Thomas Posch,Albin Alfreider,Ruben Sommaruga,Martin W Hahn,Bettina Sonntag,Purificacion Lopez-Garcia,David Moreira,Ludwig Jardillier,Cécile Lepère,Corinne Biderre-Petit,Anna Bednarska,Mirosław Ślusarczyk,Viktor R Tóth,Horia L Banciu,Konstantinos Kormas,Sandi Orlic,Danijela Šantić,Gerard Muyzer,Daniel P R Herlemann,Helen Tammert,Stefan Bertilsson,Silke Langenheder,Thomas Zechmeister,Nico Salmaso,Nicola Storelli,Camilla Capelli,Fabio Lepori,Vojtěch Lanta,Helena Henriques Vieira,Fran Kostanjšek,Kateřina Kabeláčová,Maria-Cecilia Chiriac,Markus Haber,Tanja Shabarova,Clafy Fernandes,Pavel Rychtecký,Petr Znachor,Tiberiu Szőke-Nagy,Paul Layoun,Hon Lun Wong,Vinicius Silva Kavagutti,Paul-Adrian Bulzu,Michaela M Salcher,Kasia Piwosz,Rohit Ghai
{"title":"Telonemia 原生动物的全球淡水分布。","authors":"Roudaina Boukheloua,Indranil Mukherjee,Hongjae Park,Karel Šimek,Vojtěch Kasalický,Maxon Ngochera,Hans-Peter Grossart,Antonio Picazo-Mozo,Antonio Camacho,Pedro J Cabello-Yeves,Francisco Rodriguez-Valera,Cristiana Callieri,Adrian-Stefan Andrei,Jakob Pernthaler,Thomas Posch,Albin Alfreider,Ruben Sommaruga,Martin W Hahn,Bettina Sonntag,Purificacion Lopez-Garcia,David Moreira,Ludwig Jardillier,Cécile Lepère,Corinne Biderre-Petit,Anna Bednarska,Mirosław Ślusarczyk,Viktor R Tóth,Horia L Banciu,Konstantinos Kormas,Sandi Orlic,Danijela Šantić,Gerard Muyzer,Daniel P R Herlemann,Helen Tammert,Stefan Bertilsson,Silke Langenheder,Thomas Zechmeister,Nico Salmaso,Nicola Storelli,Camilla Capelli,Fabio Lepori,Vojtěch Lanta,Helena Henriques Vieira,Fran Kostanjšek,Kateřina Kabeláčová,Maria-Cecilia Chiriac,Markus Haber,Tanja Shabarova,Clafy Fernandes,Pavel Rychtecký,Petr Znachor,Tiberiu Szőke-Nagy,Paul Layoun,Hon Lun Wong,Vinicius Silva Kavagutti,Paul-Adrian Bulzu,Michaela M Salcher,Kasia Piwosz,Rohit Ghai","doi":"10.1093/ismejo/wrae177","DOIUrl":null,"url":null,"abstract":"Telonemia are one of the oldest identified marine protists that for most part of their history have been recognized as a distinct incertae sedis lineage. Today, their evolutionary proximity to the SAR supergroup (Stramenopiles, Alveolates, and Rhizaria) is firmly established. However, their ecological distribution and importance as a natural predatory flagellate, especially in freshwater food webs, still remains unclear. To unravel the distribution and diversity of the phylum Telonemia in freshwater habitats, we examined over a thousand freshwater metagenomes from all over the world. In addition, to directly quantify absolute abundances, we analysed 407 samples from 97 lakes and reservoirs using Catalyzed Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH). We recovered Telonemia 18S rRNA gene sequences from hundreds of metagenomic samples from a wide variety of habitats, indicating a global distribution of this phylum. However, even after this extensive sampling, our phylogenetic analysis did not reveal any new major clades, suggesting current molecular surveys are near to capturing the full diversity within this group. We observed excellent concordance between CARD-FISH analyses and estimates of abundances from metagenomes. Both approaches suggest that Telonemia are largely absent from shallow lakes and prefer to inhabit the colder hypolimnion of lakes and reservoirs in the Northern Hemisphere, where they frequently bloom, reaching 10-20% of the total heterotrophic flagellate population, making them important predatory flagellates in the freshwater food web.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Global freshwater distribution of Telonemia protists.\",\"authors\":\"Roudaina Boukheloua,Indranil Mukherjee,Hongjae Park,Karel Šimek,Vojtěch Kasalický,Maxon Ngochera,Hans-Peter Grossart,Antonio Picazo-Mozo,Antonio Camacho,Pedro J Cabello-Yeves,Francisco Rodriguez-Valera,Cristiana Callieri,Adrian-Stefan Andrei,Jakob Pernthaler,Thomas Posch,Albin Alfreider,Ruben Sommaruga,Martin W Hahn,Bettina Sonntag,Purificacion Lopez-Garcia,David Moreira,Ludwig Jardillier,Cécile Lepère,Corinne Biderre-Petit,Anna Bednarska,Mirosław Ślusarczyk,Viktor R Tóth,Horia L Banciu,Konstantinos Kormas,Sandi Orlic,Danijela Šantić,Gerard Muyzer,Daniel P R Herlemann,Helen Tammert,Stefan Bertilsson,Silke Langenheder,Thomas Zechmeister,Nico Salmaso,Nicola Storelli,Camilla Capelli,Fabio Lepori,Vojtěch Lanta,Helena Henriques Vieira,Fran Kostanjšek,Kateřina Kabeláčová,Maria-Cecilia Chiriac,Markus Haber,Tanja Shabarova,Clafy Fernandes,Pavel Rychtecký,Petr Znachor,Tiberiu Szőke-Nagy,Paul Layoun,Hon Lun Wong,Vinicius Silva Kavagutti,Paul-Adrian Bulzu,Michaela M Salcher,Kasia Piwosz,Rohit Ghai\",\"doi\":\"10.1093/ismejo/wrae177\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Telonemia are one of the oldest identified marine protists that for most part of their history have been recognized as a distinct incertae sedis lineage. Today, their evolutionary proximity to the SAR supergroup (Stramenopiles, Alveolates, and Rhizaria) is firmly established. However, their ecological distribution and importance as a natural predatory flagellate, especially in freshwater food webs, still remains unclear. To unravel the distribution and diversity of the phylum Telonemia in freshwater habitats, we examined over a thousand freshwater metagenomes from all over the world. In addition, to directly quantify absolute abundances, we analysed 407 samples from 97 lakes and reservoirs using Catalyzed Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH). We recovered Telonemia 18S rRNA gene sequences from hundreds of metagenomic samples from a wide variety of habitats, indicating a global distribution of this phylum. However, even after this extensive sampling, our phylogenetic analysis did not reveal any new major clades, suggesting current molecular surveys are near to capturing the full diversity within this group. We observed excellent concordance between CARD-FISH analyses and estimates of abundances from metagenomes. Both approaches suggest that Telonemia are largely absent from shallow lakes and prefer to inhabit the colder hypolimnion of lakes and reservoirs in the Northern Hemisphere, where they frequently bloom, reaching 10-20% of the total heterotrophic flagellate population, making them important predatory flagellates in the freshwater food web.\",\"PeriodicalId\":516554,\"journal\":{\"name\":\"The ISME Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The ISME Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1093/ismejo/wrae177\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The ISME Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/ismejo/wrae177","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

Telonemia 是已发现的最古老的海洋原生生物之一,在其历史的大部分时间里,一直被认为是一个独特的非定居系。如今,它们在进化上与 SAR 超群(Stramenopiles、Alveolates 和 Rhizaria)的亲缘关系已得到牢固确立。然而,它们的生态分布以及作为自然界捕食性鞭毛虫的重要性(尤其是在淡水食物网中)仍不清楚。为了揭示淡水生境中 Telonemia 门的分布和多样性,我们研究了来自世界各地的一千多个淡水元基因组。此外,为了直接量化绝对丰度,我们使用催化报告沉积-荧光原位杂交(CARD-FISH)技术分析了来自 97 个湖泊和水库的 407 份样本。我们从来自不同生境的数百个元基因组样本中恢复了 Telonemia 18S rRNA 基因序列,这表明该菌门分布于全球。然而,即使进行了如此广泛的取样,我们的系统发育分析也没有发现任何新的主要支系,这表明目前的分子调查还接近于捕捉到该门类的全部多样性。我们观察到,CARD-FISH 分析与元基因组对丰度的估计之间存在极好的一致性。这两种方法都表明,浅水湖泊中基本上没有 Telonemia,它们更喜欢栖息在北半球湖泊和水库较冷的下盐层中,在那里它们经常大量繁殖,数量达到异养鞭毛虫总数的 10-20%,成为淡水食物网中重要的捕食性鞭毛虫。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Global freshwater distribution of Telonemia protists.
Telonemia are one of the oldest identified marine protists that for most part of their history have been recognized as a distinct incertae sedis lineage. Today, their evolutionary proximity to the SAR supergroup (Stramenopiles, Alveolates, and Rhizaria) is firmly established. However, their ecological distribution and importance as a natural predatory flagellate, especially in freshwater food webs, still remains unclear. To unravel the distribution and diversity of the phylum Telonemia in freshwater habitats, we examined over a thousand freshwater metagenomes from all over the world. In addition, to directly quantify absolute abundances, we analysed 407 samples from 97 lakes and reservoirs using Catalyzed Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH). We recovered Telonemia 18S rRNA gene sequences from hundreds of metagenomic samples from a wide variety of habitats, indicating a global distribution of this phylum. However, even after this extensive sampling, our phylogenetic analysis did not reveal any new major clades, suggesting current molecular surveys are near to capturing the full diversity within this group. We observed excellent concordance between CARD-FISH analyses and estimates of abundances from metagenomes. Both approaches suggest that Telonemia are largely absent from shallow lakes and prefer to inhabit the colder hypolimnion of lakes and reservoirs in the Northern Hemisphere, where they frequently bloom, reaching 10-20% of the total heterotrophic flagellate population, making them important predatory flagellates in the freshwater food web.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信