{"title":"采用光照优化策略,提高利用浓缩多底物原料进行光发酵的水发罗杆菌 KKU-PS1 的氢气生产率和光转换效率","authors":"","doi":"10.1016/j.ijhydene.2024.09.132","DOIUrl":null,"url":null,"abstract":"<div><p>With the use of biotechnology, hydrogen can be produced from wastewater rich in short-chain fatty acids. A previous study revealed the ability of <em>Rhodobacter sphaeroides</em> KKU-PS1 to produce biohydrogen from substrates mimicking succinate fermentation effluent. However, the process still requires optimization. Before illumination optimization, due to high concentration of the effluent, various effluent dilution factors ranging from 10 to 100 were compared, and the optimal dilution factor was determined to be 50. Light-emitting diode (LED) setups consisting of bands and tubes were compared, and various illuminated surface-to-volume ratios (S/V) were obtained. LED tubes were subsequently used for light intensity optimization in the range of 5–23 klux, revealing optimum light intensity at 15 klux, yielding 2202 mL H<sub>2</sub>/L and 13.8 mL H<sub>2</sub>/L/h as the cumulative hydrogen and maximum output rate, respectively. The lighting protocol at 15 klux and with a 6h–6h light-dark cycle improved the total light conversion efficiency by up to 3.1%. The study successfully optimized the process, with results rivalling those of a previous study using malate.</p></div>","PeriodicalId":337,"journal":{"name":"International Journal of Hydrogen Energy","volume":null,"pages":null},"PeriodicalIF":8.1000,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock\",\"authors\":\"\",\"doi\":\"10.1016/j.ijhydene.2024.09.132\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>With the use of biotechnology, hydrogen can be produced from wastewater rich in short-chain fatty acids. A previous study revealed the ability of <em>Rhodobacter sphaeroides</em> KKU-PS1 to produce biohydrogen from substrates mimicking succinate fermentation effluent. However, the process still requires optimization. Before illumination optimization, due to high concentration of the effluent, various effluent dilution factors ranging from 10 to 100 were compared, and the optimal dilution factor was determined to be 50. Light-emitting diode (LED) setups consisting of bands and tubes were compared, and various illuminated surface-to-volume ratios (S/V) were obtained. LED tubes were subsequently used for light intensity optimization in the range of 5–23 klux, revealing optimum light intensity at 15 klux, yielding 2202 mL H<sub>2</sub>/L and 13.8 mL H<sub>2</sub>/L/h as the cumulative hydrogen and maximum output rate, respectively. The lighting protocol at 15 klux and with a 6h–6h light-dark cycle improved the total light conversion efficiency by up to 3.1%. The study successfully optimized the process, with results rivalling those of a previous study using malate.</p></div>\",\"PeriodicalId\":337,\"journal\":{\"name\":\"International Journal of Hydrogen Energy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":8.1000,\"publicationDate\":\"2024-09-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Hydrogen Energy\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0360319924038412\",\"RegionNum\":2,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Hydrogen Energy","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0360319924038412","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
摘要
利用生物技术,可以从富含短链脂肪酸的废水中生产氢气。之前的一项研究表明,水合罗杆菌 KKU-PS1 能够利用模拟琥珀酸发酵废水的底物生产生物氢。然而,该过程仍需优化。在优化照明之前,由于废水浓度较高,对 10 至 100 的各种废水稀释因子进行了比较,确定最佳稀释因子为 50。比较了由灯带和灯管组成的发光二极管(LED)装置,得出了不同的照明表面体积比(S/V)。随后,在 5-23 klux 范围内对 LED 灯管进行了光强优化,发现 15 klux 时光强最佳,累积氢气和最大输出率分别为 2202 mL H2/L 和 13.8 mL H2/L/h。在 15 klux 和 6 小时-6 小时的光-暗循环下的光照方案将总光转换效率提高了 3.1%。这项研究成功地优化了工艺,其结果可与之前使用苹果酸盐的研究相媲美。
Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock
With the use of biotechnology, hydrogen can be produced from wastewater rich in short-chain fatty acids. A previous study revealed the ability of Rhodobacter sphaeroides KKU-PS1 to produce biohydrogen from substrates mimicking succinate fermentation effluent. However, the process still requires optimization. Before illumination optimization, due to high concentration of the effluent, various effluent dilution factors ranging from 10 to 100 were compared, and the optimal dilution factor was determined to be 50. Light-emitting diode (LED) setups consisting of bands and tubes were compared, and various illuminated surface-to-volume ratios (S/V) were obtained. LED tubes were subsequently used for light intensity optimization in the range of 5–23 klux, revealing optimum light intensity at 15 klux, yielding 2202 mL H2/L and 13.8 mL H2/L/h as the cumulative hydrogen and maximum output rate, respectively. The lighting protocol at 15 klux and with a 6h–6h light-dark cycle improved the total light conversion efficiency by up to 3.1%. The study successfully optimized the process, with results rivalling those of a previous study using malate.
期刊介绍:
The objective of the International Journal of Hydrogen Energy is to facilitate the exchange of new ideas, technological advancements, and research findings in the field of Hydrogen Energy among scientists and engineers worldwide. This journal showcases original research, both analytical and experimental, covering various aspects of Hydrogen Energy. These include production, storage, transmission, utilization, enabling technologies, environmental impact, economic considerations, and global perspectives on hydrogen and its carriers such as NH3, CH4, alcohols, etc.
The utilization aspect encompasses various methods such as thermochemical (combustion), photochemical, electrochemical (fuel cells), and nuclear conversion of hydrogen, hydrogen isotopes, and hydrogen carriers into thermal, mechanical, and electrical energies. The applications of these energies can be found in transportation (including aerospace), industrial, commercial, and residential sectors.