发情早期卵泡内注射 PGE2 或 PGF2α 对母马排卵、出血性无排卵卵泡形成、孕酮分泌和妊娠结果的影响

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY
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引用次数: 0

摘要

本实验旨在评估在发情早期对母马卵泡内注射 PGE2 或 PGF2α 是否能诱导正常排卵、孕酮分泌(实验 1)和妊娠(实验 2)。在实验 1 中,发情母马在子宫内膜水肿 2 天后,在所有最大的优势卵泡(直径 28-35 mm)中注射 0.5 mL 含有 500 μg PGE2(n = 6)、125 μg PGF2α (n = 6)或安慰剂(n = 7)的无菌水(第 0 小时)。每天进行超声波检查,直到检测到排卵或无排卵,并在 8 天内每天采集血液样本。在实验 2 中,对至少三天后出现轻度至中度子宫内膜水肿且优势卵泡≥35 mm 的母马注射 500 μg PGE2(PGE2 组;n = 9 头母马和 11 个优势卵泡),稀释在 0.5 mL 无菌水中,注射到卵泡中(PGE2 组;n = 9 头母马和 11 个优势卵泡)。对照组(n = 9 头母马和 11 个优势卵泡)不进行穿刺。两组母马都进行了人工授精。在实验 1 中,PGE2 组的所有母马(6/6)均在治疗后 24 小时内排卵。PGE2 组母马从卵泡内注射到排卵的平均时间间隔(24 ± 0 h)比对照组母马(77 ± 9 h)短(P < 0.001)。PGF2α 组母马出现出血性无排卵卵泡(HAF)的频率(7/7)高于对照组母马(2/7);P < 0.05)。在排卵后早期,PGF2α组母马的孕酮浓度低于对照组母马(P < 0.004)。与 PGF2α 组和 PGE2 组母马相比,对照组母马排卵后孕酮浓度首次显著增加的时间更早(P < 0.05)。在实验 2 中,对照组(7/9,78%)比 PGE2 组(2/9,22%)更多的母马怀孕(P = 0.015)。总之,单用 PGE2 可在给药后 24 小时内诱导所有受试母马的卵泡破裂,而 PGF2α 可阻止排卵并诱导形成 HAF。然而,与对照组母马相比,PGE2诱导排卵的母马排卵后孕酮分泌上升延迟,繁殖力降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of intrafollicular administration of PGE2 or PGF2α in early estrus on ovulation, hemorrhagic anovulatory follicles formation, progesterone secretion and pregnancy outcome in the mare

This experiment was performed to evaluate whether intrafollicular treatment of PGE2 or PGF2α administered in early estrus would induce normal ovulation, progesterone production (Experiment 1) and pregnancy (Experiment 2). In Experiment 1, mares in estrus after 2 days of endometrial edema were injected in all largest dominant follicles (28–35 mm in diameter) with 0.5 mL of sterile water containing 500 μg PGE2 (n = 6), 125 μg PGF2α (n = 6) or placebo (n = 7) (Hour 0). Ultrasound examinations were performed daily, until ovulation or anovulation was detected, and daily blood samples were taken for 8 days. In Experiment 2, mares with a dominant follicle ≥35 mm after at least three days of slight-to-moderate endometrial edema, were injected with 500 μg PGE2 diluted in 0.5 mL of sterile water for injection in the follicle (PGE2 group; n = 9 mares and 11 dominant follicles). No puncture was performed in the control group (n = 9 mares and 11 dominant follicles). Mares from both groups were inseminated. In Experiment 1, all mares (6/6) in the PGE2 group ovulated within 24 h of treatment. The mean interval from intrafollicular injection to ovulation was shorter (P < 0.001) in PGE2 mares (24 ± 0 h) than in control mares (77 ± 9 h). Mares from the PGF2α group developed hemorrhagic anovulatory follicles (HAF) more often (7/7) than control mares (2/7); P < 0.05). The progesterone concentration in mares from the PGF2α group was lower (P < 0.004) than control mares in the early post-ovulatory period. The first significant increase in post-ovulatory progesterone concentration occurred earlier (P < 0.05) in mares from the control group than in mares from the PGF2α and PGE2 groups. In Experiment 2, more mares from the control group (7/9, 78 %) became pregnant than from the PGE2 group (2/9, 22 %) (P = 0.015). In conclusion, PGE2 alone induced follicle collapse in all treated mares within 24 h of administrations, while PGF2α blocked ovulation and induced formation of HAFs. However, the post-ovulatory rise in progesterone production was delayed and the fertility reduced in mares with ovulation induced by PGE2 compared to control mares.

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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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