{"title":"棉籽粕蛋白水解物对黄羽肉鸡肠道微生物群的影响","authors":"Xiaoyang Zhang, Hailiang Wang, Yujie Niu, Cheng Chen, Wenju Zhang","doi":"10.3389/fmicb.2024.1434252","DOIUrl":null,"url":null,"abstract":"We evaluated the effects of cottonseed meal protein hydrolysate (CPH) on the intestinal microbiota of yellow-feather broilers. We randomly divided 240 chicks into four groups with six replicates: basal diet with 0% (CON), 1% (LCPH), 3% (MCPH), or 5% (HCPH) CPH. The test lasted 63 days and included days 1–21, 22–42, and 43–63 phases. The ACE, Chao1, and Shannon indices in the MCPH and HCPH groups of 42-day-old broilers were higher than those in the CON group (<jats:italic>p</jats:italic> &lt; 0.05), indicating that the cecum microbial diversity and richness were higher in these groups. <jats:italic>Firmicutes</jats:italic> and <jats:italic>Bacteroidetes</jats:italic> were the dominant phyla; however, the main genera varied during the different periods. The abundance of <jats:italic>Lactobacillus</jats:italic> in CPH treatment groups of 21-day-old broilers was high (<jats:italic>p</jats:italic> &lt; 0.05); in the 42-day-old broilers, the abundances of <jats:italic>Barnesiella</jats:italic>, <jats:italic>Clostridia_vadinBB60_group</jats:italic>, and <jats:italic>Parasutterella</jats:italic> in the LCPH group, <jats:italic>Desulfovibrio</jats:italic>, <jats:italic>Lactobacillus</jats:italic>, <jats:italic>Clostridia_vadinBB60_group</jats:italic>, and <jats:italic>Butyricicoccus</jats:italic> in the MCPH group, and <jats:italic>Megamonas</jats:italic> and <jats:italic>Streptococcus</jats:italic> in the HCPH group increased; in the 63-day-old broilers, the abundance of <jats:italic>Clostridia_UCG-014</jats:italic> and <jats:italic>Synergistes</jats:italic> in the LCPH and HCPH group, respectively, increased (<jats:italic>p</jats:italic> &lt; 0.05), and that of <jats:italic>Alistipes</jats:italic> in the LCPH and MCPH groups decreased (<jats:italic>p</jats:italic> &lt; 0.05). And changes in the abundance of probiotics were beneficial to improve the intestinal morphology and growth performance. In addition, the LCPH treatment increased the complexity of the microbial network, while the MCPH treatment had the same effect in 42-day-old broilers. Thus, CPH increased the relative abundance of beneficial intestinal microbiota and enhanced the richness and diversity of the bacterial microbiota in broilers aged &lt;42 days; this effect was weakened after 42 days.","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of cottonseed meal protein hydrolysate on intestinal microbiota of yellow-feather broilers\",\"authors\":\"Xiaoyang Zhang, Hailiang Wang, Yujie Niu, Cheng Chen, Wenju Zhang\",\"doi\":\"10.3389/fmicb.2024.1434252\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We evaluated the effects of cottonseed meal protein hydrolysate (CPH) on the intestinal microbiota of yellow-feather broilers. We randomly divided 240 chicks into four groups with six replicates: basal diet with 0% (CON), 1% (LCPH), 3% (MCPH), or 5% (HCPH) CPH. The test lasted 63 days and included days 1–21, 22–42, and 43–63 phases. The ACE, Chao1, and Shannon indices in the MCPH and HCPH groups of 42-day-old broilers were higher than those in the CON group (<jats:italic>p</jats:italic> &lt; 0.05), indicating that the cecum microbial diversity and richness were higher in these groups. <jats:italic>Firmicutes</jats:italic> and <jats:italic>Bacteroidetes</jats:italic> were the dominant phyla; however, the main genera varied during the different periods. The abundance of <jats:italic>Lactobacillus</jats:italic> in CPH treatment groups of 21-day-old broilers was high (<jats:italic>p</jats:italic> &lt; 0.05); in the 42-day-old broilers, the abundances of <jats:italic>Barnesiella</jats:italic>, <jats:italic>Clostridia_vadinBB60_group</jats:italic>, and <jats:italic>Parasutterella</jats:italic> in the LCPH group, <jats:italic>Desulfovibrio</jats:italic>, <jats:italic>Lactobacillus</jats:italic>, <jats:italic>Clostridia_vadinBB60_group</jats:italic>, and <jats:italic>Butyricicoccus</jats:italic> in the MCPH group, and <jats:italic>Megamonas</jats:italic> and <jats:italic>Streptococcus</jats:italic> in the HCPH group increased; in the 63-day-old broilers, the abundance of <jats:italic>Clostridia_UCG-014</jats:italic> and <jats:italic>Synergistes</jats:italic> in the LCPH and HCPH group, respectively, increased (<jats:italic>p</jats:italic> &lt; 0.05), and that of <jats:italic>Alistipes</jats:italic> in the LCPH and MCPH groups decreased (<jats:italic>p</jats:italic> &lt; 0.05). And changes in the abundance of probiotics were beneficial to improve the intestinal morphology and growth performance. In addition, the LCPH treatment increased the complexity of the microbial network, while the MCPH treatment had the same effect in 42-day-old broilers. Thus, CPH increased the relative abundance of beneficial intestinal microbiota and enhanced the richness and diversity of the bacterial microbiota in broilers aged &lt;42 days; this effect was weakened after 42 days.\",\"PeriodicalId\":12466,\"journal\":{\"name\":\"Frontiers in Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3389/fmicb.2024.1434252\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fmicb.2024.1434252","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Effects of cottonseed meal protein hydrolysate on intestinal microbiota of yellow-feather broilers
We evaluated the effects of cottonseed meal protein hydrolysate (CPH) on the intestinal microbiota of yellow-feather broilers. We randomly divided 240 chicks into four groups with six replicates: basal diet with 0% (CON), 1% (LCPH), 3% (MCPH), or 5% (HCPH) CPH. The test lasted 63 days and included days 1–21, 22–42, and 43–63 phases. The ACE, Chao1, and Shannon indices in the MCPH and HCPH groups of 42-day-old broilers were higher than those in the CON group (p < 0.05), indicating that the cecum microbial diversity and richness were higher in these groups. Firmicutes and Bacteroidetes were the dominant phyla; however, the main genera varied during the different periods. The abundance of Lactobacillus in CPH treatment groups of 21-day-old broilers was high (p < 0.05); in the 42-day-old broilers, the abundances of Barnesiella, Clostridia_vadinBB60_group, and Parasutterella in the LCPH group, Desulfovibrio, Lactobacillus, Clostridia_vadinBB60_group, and Butyricicoccus in the MCPH group, and Megamonas and Streptococcus in the HCPH group increased; in the 63-day-old broilers, the abundance of Clostridia_UCG-014 and Synergistes in the LCPH and HCPH group, respectively, increased (p < 0.05), and that of Alistipes in the LCPH and MCPH groups decreased (p < 0.05). And changes in the abundance of probiotics were beneficial to improve the intestinal morphology and growth performance. In addition, the LCPH treatment increased the complexity of the microbial network, while the MCPH treatment had the same effect in 42-day-old broilers. Thus, CPH increased the relative abundance of beneficial intestinal microbiota and enhanced the richness and diversity of the bacterial microbiota in broilers aged <42 days; this effect was weakened after 42 days.
期刊介绍:
Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.