批准基于成熟小鼠树突状细胞的抗肿瘤疫苗同源模型，以研究细胞产品的生物分布情况

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-17 DOI:10.1007/s10517-024-06226-5

T. L. Nekhaeva, I. D. Laskov, E. I. Fedoros, A. B. Danilova, M. N. Yurova, M. L. Tyndyk, E. D. Ermakova, N. V. Emelyanova, N. A. Efremova, A. V. Grigorevskaya, M. A. Nekrasova, I. A. Baldueva

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引用次数: 0

摘要

根据为雌性 BALB/c 小鼠制定的方案获得同源动物细胞产品。评估了树突状细胞（DC）从注射部位向引流淋巴结的迁移。引流淋巴结中用羧基荧光素琥珀酰亚胺酯（CFSE）标记的树突状细胞数量从5.3%（16小时）增加到13.3%（48小时）（p=0.028），72小时达到最大值（15.4%，p=0.003）。在小鼠淋巴结中检测到的 CFSE-DC 的免疫表型与成熟疫苗 DC 的免疫表型一致：它们表达分化标记 CD11c、CD80、CD83 和 CD86（与初始 DC 相比，p>0.05）。

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Approbation of a Homologous Model of the Antitumor Vaccine Based on Mature Mouse Dendritic Cells to Study the Biodistribution of the Cell Product

Homologous animal cell product was obtained in protocol developed for female BALB/c mice. Dendritic cell (DC) migration from the injection site into the draining lymph nodes was evaluated. The number of DC labeled with carboxyfluorescein succinimidyl ester (CFSE) in draining lymph nodes increased from 5.3% (16 h) to 13.3% (48 h) (p=0.028) with a maximum at 72 h (15.4%, p=0.003). The immunophenotype of CFSE-DC detected in murine lymph nodes corresponded to the immunophenotype of mature vaccine DCs: they expressed differentiation markers CD11c, CD80, CD83, and CD86 (p>0.05 vs initial DC).

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.