负载羟基磷灰石纳米颗粒的琼脂/羧甲基纤维素复合薄膜用于骨再生

IF 5.4 3区 材料科学 Q2 CHEMISTRY, PHYSICAL
Kiseok Han, Anbazhagan Sathiyaseelan, Yuting Lu, Kristine M. Kim, Myeong-Hyeon Wang
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引用次数: 0

摘要

治疗骨缺损的生物材料工程方法包括利用强效生物活性分子的组合来刺激细胞增殖,为再生过程创造有利的环境和支架。由于全球人口老龄化,骨再生和伤口愈合方面的研究迫在眉睫。羟基磷灰石(HAP)是骨组织的主要矿物成分,具有很高的生物相容性和生物活性。琼脂和羧甲基纤维素(CMC)都具有生物材料的基本特征,可以单独使用,也可以混合使用。因此,本研究旨在制备负载 HAP 纳米粒子的琼脂/CMC 复合薄膜,以增强骨再生应用。研究采用 XRD、SEM、TGA 和 FTIR 对薄膜复合材料的晶体结构、形态、相组成、热稳定性和化学状态进行了表征。对大鼠成纤维细胞进行的细胞毒性评估表明,薄膜复合材料的生物相容性超过 90%。此外,在伤口愈合实验中,纳米复合膜处理组(98.14 ± 0.15%)的伤口闭合率比阴性对照组(62.08 ± 1.87%)高出 35%。茜素红染色检测显示,与阴性对照组相比,经处理的 MC3T3-E1 细胞的钙沉积增加了 20.89 ± 6.9%,这肯定了它们的成骨潜力。这些结果表明,所开发的纳米复合薄膜是一种很有前景的治疗平台,可有效解决复杂的骨相关疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Agar/carboxymethyl cellulose composite film loaded with hydroxyapatite nanoparticles for bone regeneration

Agar/carboxymethyl cellulose composite film loaded with hydroxyapatite nanoparticles for bone regeneration

Biomaterials engineering approaches for treating bone defects involve utilizing a combination of potent bioactive molecules to stimulate cell proliferation, fostering a conducive environment and scaffold for the regeneration process. Due to the aging global population, there is an urgent need for research in bone regeneration and wound healing. Hydroxyapatite (HAP) is a major mineral component of bone tissue with high biocompatibility and bioactivity. Agar and carboxymethyl cellulose (CMC) both exhibit the essential characteristics of biomaterials, either separately or in combination. Hence, this present study aimed to prepare HAP nanoparticles loaded Agar/CMC composite film for enhanced bone regenerative applications. The crystal structure, morphology, phase composition, thermal stability, and chemical state of the film composites were characterized using XRD, SEM, TGA, and FTIR. Cytotoxicity evaluation on rat fibroblasts cells indicated over 90% biocompatibility for the film composites. Moreover, in wound healing assays, the nanocomposite film-treated group (98.14 ± 0.15%) exhibited a 35% higher wound closure rate compared to the negative control group (62.08 ± 1.87%). Alizarin Red Staining assay revealed a 20.89 ± 6.9% increase in calcium deposition in treated MC3T3-E1 cells compared to the negative control, affirming their osteogenic potential. These results demonstrate that the developed nanocomposite film is a promising therapeutic platform for effectively addressing complex bone-related ailments.

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来源期刊
ACS Applied Energy Materials
ACS Applied Energy Materials Materials Science-Materials Chemistry
CiteScore
10.30
自引率
6.20%
发文量
1368
期刊介绍: ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.
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