亚临床坏死性肠炎挑战期间补充精氨酸对肉鸡肠道微生物稳态的影响

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Shahna Fathima, Walid G. Al Hakeem, Revathi Shanmugasundaram, Jeferson Lourenco, Ramesh K. Selvaraj
{"title":"亚临床坏死性肠炎挑战期间补充精氨酸对肉鸡肠道微生物稳态的影响","authors":"Shahna Fathima, Walid G. Al Hakeem, Revathi Shanmugasundaram, Jeferson Lourenco, Ramesh K. Selvaraj","doi":"10.3389/fphys.2024.1463420","DOIUrl":null,"url":null,"abstract":"Necrotic enteritis (NE) is an enteric disease of poultry that alters the structure of the gut microbial community causing dysbiosis. This 28 day experiment investigated the effects of 125% and 135% arginine diets on the gut microbial diversity and composition of broilers during a subclinical NE challenge. One hundred and twenty one-day-old chicks were randomly allocated to 4 treatments with six replicates each- Uninfected + Basal, NE + Basal, NE + Arg 125%, and NE + Arg 135% diet groups. NE was induced by inoculating 1 × 10<jats:sup>4</jats:sup> <jats:italic>E. maxima</jats:italic> sporulated oocysts on day 14 and 1 × 10<jats:sup>8</jats:sup> CFU <jats:italic>C. perfringens</jats:italic> on days 19, 20, and 21 of age. The NE challenge significantly decreased the number of observed amplicon sequence variants (<jats:italic>p</jats:italic> = 0.03), the abundance of the phylum Firmicutes (<jats:italic>p</jats:italic> &amp;lt; 0.01), and the species <jats:italic>Mediterraneibacter cottocaccae</jats:italic> (<jats:italic>p</jats:italic> = 0.01) in the ceca of birds on day 21. The NE challenge significantly increased the Bray-Curtis index (<jats:italic>p</jats:italic> &amp;lt; 0.01), and the abundance of the phylum Bacteroidota (<jats:italic>p</jats:italic> &amp;lt; 0.01), family Odoribacteraceae (<jats:italic>p</jats:italic> &amp;lt; 0.01), genus <jats:italic>Odoribacter</jats:italic> (<jats:italic>p</jats:italic> &amp;lt; 0.01), and species <jats:italic>O. splanchnicus</jats:italic> (<jats:italic>p</jats:italic> = 0.01) on day 21. During NE, the 125% arginine diet restored the abundance of the phylum Bacteroidota (<jats:italic>p</jats:italic> = 0.03), family Odoribacteraceae (<jats:italic>p</jats:italic> = 0.03) and Oscillospiraceae (<jats:italic>p</jats:italic> = 0.03), genus <jats:italic>Odoribacter</jats:italic> (<jats:italic>p</jats:italic> = 0.03), and species <jats:italic>O. splanchnicus</jats:italic> (<jats:italic>p</jats:italic> = 0.03) and <jats:italic>M. cottocaccae</jats:italic> (<jats:italic>p</jats:italic> &amp;lt; 0.01) on day 21. The 135% arginine diet effectively restored the loss in alpha diversity (<jats:italic>p</jats:italic> = 0.01) caused by NE, the abundance of the phylum Firmicutes (<jats:italic>p</jats:italic> = 0.01) and Bacteroidota (<jats:italic>p</jats:italic> &amp;lt; 0.01), family Oscillospiraceae (<jats:italic>p</jats:italic> = 0.03) and Odoribacteraceae (<jats:italic>p</jats:italic> &amp;lt; 0.01), genus <jats:italic>Odoribacter</jats:italic> (<jats:italic>p</jats:italic> &amp;lt; 0.01), and species <jats:italic>O. splanchnicus</jats:italic> (<jats:italic>p</jats:italic> &amp;lt; 0.01) and <jats:italic>M. cottocaccae</jats:italic> (<jats:italic>p</jats:italic> &amp;lt; 0.01) on day 21. On day 28, the treatments had a significant effect on the cecal propionate (<jats:italic>p</jats:italic> = 0.01), butyrate (<jats:italic>p</jats:italic> = 0.04), and total SCFA (<jats:italic>p</jats:italic> = 0.04) concentrations. In conclusion, the 125% and 135% arginine diets restored gut microbial composition during a subclinical NE challenge, but not the cecal SCFA profile. Hence, arginine in combination with other feed additives could be used in restoring gut microbial homeostasis during NE in poultry.","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effect of supplemental arginine on the gut microbial homeostasis of broilers during sub-clinical necrotic enteritis challenge\",\"authors\":\"Shahna Fathima, Walid G. Al Hakeem, Revathi Shanmugasundaram, Jeferson Lourenco, Ramesh K. Selvaraj\",\"doi\":\"10.3389/fphys.2024.1463420\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Necrotic enteritis (NE) is an enteric disease of poultry that alters the structure of the gut microbial community causing dysbiosis. This 28 day experiment investigated the effects of 125% and 135% arginine diets on the gut microbial diversity and composition of broilers during a subclinical NE challenge. One hundred and twenty one-day-old chicks were randomly allocated to 4 treatments with six replicates each- Uninfected + Basal, NE + Basal, NE + Arg 125%, and NE + Arg 135% diet groups. NE was induced by inoculating 1 × 10<jats:sup>4</jats:sup> <jats:italic>E. maxima</jats:italic> sporulated oocysts on day 14 and 1 × 10<jats:sup>8</jats:sup> CFU <jats:italic>C. perfringens</jats:italic> on days 19, 20, and 21 of age. The NE challenge significantly decreased the number of observed amplicon sequence variants (<jats:italic>p</jats:italic> = 0.03), the abundance of the phylum Firmicutes (<jats:italic>p</jats:italic> &amp;lt; 0.01), and the species <jats:italic>Mediterraneibacter cottocaccae</jats:italic> (<jats:italic>p</jats:italic> = 0.01) in the ceca of birds on day 21. The NE challenge significantly increased the Bray-Curtis index (<jats:italic>p</jats:italic> &amp;lt; 0.01), and the abundance of the phylum Bacteroidota (<jats:italic>p</jats:italic> &amp;lt; 0.01), family Odoribacteraceae (<jats:italic>p</jats:italic> &amp;lt; 0.01), genus <jats:italic>Odoribacter</jats:italic> (<jats:italic>p</jats:italic> &amp;lt; 0.01), and species <jats:italic>O. splanchnicus</jats:italic> (<jats:italic>p</jats:italic> = 0.01) on day 21. During NE, the 125% arginine diet restored the abundance of the phylum Bacteroidota (<jats:italic>p</jats:italic> = 0.03), family Odoribacteraceae (<jats:italic>p</jats:italic> = 0.03) and Oscillospiraceae (<jats:italic>p</jats:italic> = 0.03), genus <jats:italic>Odoribacter</jats:italic> (<jats:italic>p</jats:italic> = 0.03), and species <jats:italic>O. splanchnicus</jats:italic> (<jats:italic>p</jats:italic> = 0.03) and <jats:italic>M. cottocaccae</jats:italic> (<jats:italic>p</jats:italic> &amp;lt; 0.01) on day 21. The 135% arginine diet effectively restored the loss in alpha diversity (<jats:italic>p</jats:italic> = 0.01) caused by NE, the abundance of the phylum Firmicutes (<jats:italic>p</jats:italic> = 0.01) and Bacteroidota (<jats:italic>p</jats:italic> &amp;lt; 0.01), family Oscillospiraceae (<jats:italic>p</jats:italic> = 0.03) and Odoribacteraceae (<jats:italic>p</jats:italic> &amp;lt; 0.01), genus <jats:italic>Odoribacter</jats:italic> (<jats:italic>p</jats:italic> &amp;lt; 0.01), and species <jats:italic>O. splanchnicus</jats:italic> (<jats:italic>p</jats:italic> &amp;lt; 0.01) and <jats:italic>M. cottocaccae</jats:italic> (<jats:italic>p</jats:italic> &amp;lt; 0.01) on day 21. On day 28, the treatments had a significant effect on the cecal propionate (<jats:italic>p</jats:italic> = 0.01), butyrate (<jats:italic>p</jats:italic> = 0.04), and total SCFA (<jats:italic>p</jats:italic> = 0.04) concentrations. In conclusion, the 125% and 135% arginine diets restored gut microbial composition during a subclinical NE challenge, but not the cecal SCFA profile. Hence, arginine in combination with other feed additives could be used in restoring gut microbial homeostasis during NE in poultry.\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3389/fphys.2024.1463420\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fphys.2024.1463420","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0

摘要

坏死性肠炎 (NE) 是一种家禽肠道疾病,会改变肠道微生物群落的结构,导致菌群失调。这项为期 28 天的实验研究了 125% 和 135% 精氨酸日粮在亚临床 NE 挑战期间对肉鸡肠道微生物多样性和组成的影响。120 只一天龄的雏鸡被随机分配到 4 个处理中,每个处理有 6 个重复--未感染 + 基础日粮组、NE + 基础日粮组、NE + Arg 125% 日粮组和 NE + Arg 135% 日粮组。第 14 天接种 1 × 104 E. maxima 孢子卵囊,第 19、20 和 21 天接种 1 × 108 CFU C. perfringens,诱导 NE。NE 挑战明显降低了第 21 天鸟类盲肠中观察到的扩增子序列变异的数量(p = 0.03)、真菌门的丰度(p &lt; 0.01)以及棉状地中海杆菌的种类(p = 0.01)。NE 挑战明显增加了第 21 天鸟类盲肠中的 Bray-Curtis 指数(p & p; lt; 0.01)和类杆菌门(p & p; lt; 0.01)、Odoribacteraceae 科(p & p; lt; 0.01)、Odoribacter 属(p & p; lt; 0.01)和 O. splanchnicus 种(p = 0.01)。在 NE 期间,125% 精氨酸日粮在第 21 天恢复了类杆菌门(p = 0.03)、Odoribacteraceae(p = 0.03)和 Oscillospiraceae(p = 0.03)、Odoribacter 属(p = 0.03)、O. splanchnicus(p = 0.03)和 M. cottocaccae(p &lt; 0.01)的丰度。135% 的精氨酸日粮有效地恢复了 NE 导致的 alpha 多样性损失(p = 0.01)、真菌门(p = 0.01)和类杆菌门(p &lt; 0.01)、鹅膏菌科(p = 0.03)和臭菌科(p &lt; 0.01)、臭菌属(p &lt; 0.01)、O. splanchnicus(p &lt; 0.01)和 M. cottocaccae(p &lt; 0.01)。在第 28 天,处理对盲肠丙酸盐(p = 0.01)、丁酸盐(p = 0.04)和总 SCFA(p = 0.04)浓度有显著影响。总之,125% 和 135% 精氨酸膳食可恢复亚临床 NE 挑战期间的肠道微生物组成,但不能恢复盲肠 SCFA 图谱。因此,精氨酸与其他饲料添加剂结合可用于恢复家禽 NE 期间的肠道微生物平衡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of supplemental arginine on the gut microbial homeostasis of broilers during sub-clinical necrotic enteritis challenge
Necrotic enteritis (NE) is an enteric disease of poultry that alters the structure of the gut microbial community causing dysbiosis. This 28 day experiment investigated the effects of 125% and 135% arginine diets on the gut microbial diversity and composition of broilers during a subclinical NE challenge. One hundred and twenty one-day-old chicks were randomly allocated to 4 treatments with six replicates each- Uninfected + Basal, NE + Basal, NE + Arg 125%, and NE + Arg 135% diet groups. NE was induced by inoculating 1 × 104 E. maxima sporulated oocysts on day 14 and 1 × 108 CFU C. perfringens on days 19, 20, and 21 of age. The NE challenge significantly decreased the number of observed amplicon sequence variants (p = 0.03), the abundance of the phylum Firmicutes (p &lt; 0.01), and the species Mediterraneibacter cottocaccae (p = 0.01) in the ceca of birds on day 21. The NE challenge significantly increased the Bray-Curtis index (p &lt; 0.01), and the abundance of the phylum Bacteroidota (p &lt; 0.01), family Odoribacteraceae (p &lt; 0.01), genus Odoribacter (p &lt; 0.01), and species O. splanchnicus (p = 0.01) on day 21. During NE, the 125% arginine diet restored the abundance of the phylum Bacteroidota (p = 0.03), family Odoribacteraceae (p = 0.03) and Oscillospiraceae (p = 0.03), genus Odoribacter (p = 0.03), and species O. splanchnicus (p = 0.03) and M. cottocaccae (p &lt; 0.01) on day 21. The 135% arginine diet effectively restored the loss in alpha diversity (p = 0.01) caused by NE, the abundance of the phylum Firmicutes (p = 0.01) and Bacteroidota (p &lt; 0.01), family Oscillospiraceae (p = 0.03) and Odoribacteraceae (p &lt; 0.01), genus Odoribacter (p &lt; 0.01), and species O. splanchnicus (p &lt; 0.01) and M. cottocaccae (p &lt; 0.01) on day 21. On day 28, the treatments had a significant effect on the cecal propionate (p = 0.01), butyrate (p = 0.04), and total SCFA (p = 0.04) concentrations. In conclusion, the 125% and 135% arginine diets restored gut microbial composition during a subclinical NE challenge, but not the cecal SCFA profile. Hence, arginine in combination with other feed additives could be used in restoring gut microbial homeostasis during NE in poultry.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信