亚临床坏死性肠炎挑战期间补充精氨酸对肉鸡肠道微生物稳态的影响

IF 3.2 3区医学 Q2 PHYSIOLOGY

Frontiers in Physiology Pub Date : 2024-09-18 DOI:10.3389/fphys.2024.1463420

Shahna Fathima, Walid G. Al Hakeem, Revathi Shanmugasundaram, Jeferson Lourenco, Ramesh K. Selvaraj

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引用次数: 0

摘要

坏死性肠炎 (NE) 是一种家禽肠道疾病，会改变肠道微生物群落的结构，导致菌群失调。这项为期 28 天的实验研究了 125% 和 135% 精氨酸日粮在亚临床 NE 挑战期间对肉鸡肠道微生物多样性和组成的影响。120 只一天龄的雏鸡被随机分配到 4 个处理中，每个处理有 6 个重复--未感染 + 基础日粮组、NE + 基础日粮组、NE + Arg 125% 日粮组和 NE + Arg 135% 日粮组。第 14 天接种 1 × 104 E. maxima 孢子卵囊，第 19、20 和 21 天接种 1 × 108 CFU C. perfringens，诱导 NE。NE 挑战明显降低了第 21 天鸟类盲肠中观察到的扩增子序列变异的数量（p = 0.03）、真菌门的丰度（p < 0.01）以及棉状地中海杆菌的种类（p = 0.01）。NE 挑战明显增加了第 21 天鸟类盲肠中的 Bray-Curtis 指数（p & p; lt; 0.01）和类杆菌门（p & p; lt; 0.01）、Odoribacteraceae 科（p & p; lt; 0.01）、Odoribacter 属（p & p; lt; 0.01）和 O. splanchnicus 种（p = 0.01）。在 NE 期间，125% 精氨酸日粮在第 21 天恢复了类杆菌门（p = 0.03）、Odoribacteraceae（p = 0.03）和 Oscillospiraceae（p = 0.03）、Odoribacter 属（p = 0.03）、O. splanchnicus（p = 0.03）和 M. cottocaccae（p < 0.01）的丰度。135% 的精氨酸日粮有效地恢复了 NE 导致的 alpha 多样性损失（p = 0.01）、真菌门（p = 0.01）和类杆菌门（p < 0.01）、鹅膏菌科（p = 0.03）和臭菌科（p < 0.01）、臭菌属（p < 0.01）、O. splanchnicus（p < 0.01）和 M. cottocaccae（p < 0.01）。在第 28 天，处理对盲肠丙酸盐（p = 0.01）、丁酸盐（p = 0.04）和总 SCFA（p = 0.04）浓度有显著影响。总之，125% 和 135% 精氨酸膳食可恢复亚临床 NE 挑战期间的肠道微生物组成，但不能恢复盲肠 SCFA 图谱。因此，精氨酸与其他饲料添加剂结合可用于恢复家禽 NE 期间的肠道微生物平衡。

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The effect of supplemental arginine on the gut microbial homeostasis of broilers during sub-clinical necrotic enteritis challenge

Necrotic enteritis (NE) is an enteric disease of poultry that alters the structure of the gut microbial community causing dysbiosis. This 28 day experiment investigated the effects of 125% and 135% arginine diets on the gut microbial diversity and composition of broilers during a subclinical NE challenge. One hundred and twenty one-day-old chicks were randomly allocated to 4 treatments with six replicates each- Uninfected + Basal, NE + Basal, NE + Arg 125%, and NE + Arg 135% diet groups. NE was induced by inoculating 1 × 104 E. maxima sporulated oocysts on day 14 and 1 × 108 CFU C. perfringens on days 19, 20, and 21 of age. The NE challenge significantly decreased the number of observed amplicon sequence variants (p = 0.03), the abundance of the phylum Firmicutes (p < 0.01), and the species Mediterraneibacter cottocaccae (p = 0.01) in the ceca of birds on day 21. The NE challenge significantly increased the Bray-Curtis index (p < 0.01), and the abundance of the phylum Bacteroidota (p < 0.01), family Odoribacteraceae (p < 0.01), genus Odoribacter (p < 0.01), and species O. splanchnicus (p = 0.01) on day 21. During NE, the 125% arginine diet restored the abundance of the phylum Bacteroidota (p = 0.03), family Odoribacteraceae (p = 0.03) and Oscillospiraceae (p = 0.03), genus Odoribacter (p = 0.03), and species O. splanchnicus (p = 0.03) and M. cottocaccae (p < 0.01) on day 21. The 135% arginine diet effectively restored the loss in alpha diversity (p = 0.01) caused by NE, the abundance of the phylum Firmicutes (p = 0.01) and Bacteroidota (p < 0.01), family Oscillospiraceae (p = 0.03) and Odoribacteraceae (p < 0.01), genus Odoribacter (p < 0.01), and species O. splanchnicus (p < 0.01) and M. cottocaccae (p < 0.01) on day 21. On day 28, the treatments had a significant effect on the cecal propionate (p = 0.01), butyrate (p = 0.04), and total SCFA (p = 0.04) concentrations. In conclusion, the 125% and 135% arginine diets restored gut microbial composition during a subclinical NE challenge, but not the cecal SCFA profile. Hence, arginine in combination with other feed additives could be used in restoring gut microbial homeostasis during NE in poultry.

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来源期刊

Frontiers in Physiology PHYSIOLOGY-

CiteScore

6.50

自引率

5.00%

发文量

2608

审稿时长

14 weeks

期刊介绍： Frontiers in Physiology is a leading journal in its field, publishing rigorously peer-reviewed research on the physiology of living systems, from the subcellular and molecular domains to the intact organism, and its interaction with the environment. Field Chief Editor George E. Billman at the Ohio State University Columbus is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.