{"title":"基于生物信息学分析的 miR-625-5p 在脓毒症诱发急性肾损伤患者中的临床意义","authors":"Baoying Gong, Jiayi Chen, Haizhen Yu, Shizhen Li","doi":"10.1007/s11255-024-04209-z","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Purpose</h3><p>Sepsis often leads to a cluster of life-threatening symptoms affecting multiple organ systems. Among the organs most vulnerable to damage in this state is the kidney; those afflicted with severe sepsis frequently encounter acute kidney injury (AKI). The study was to investigate the diagnostic role of miR-625-5p in sepsis and sepsis-induced acute kidney injury (SI-AKI) and predict the possible pathways of miR-625-5p involved in SI-AKI by bioinformatics method.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>RT-qPCR was used to detect the level of miR-625-5p, and the diagnostic value of miR-625-5p was analyzed using ROC curve. The proliferation and the concentration of inflammatory factors of HK-2 cells induced by LPS were detected by CCK-8 and ELISA. The pathway of miR-625-5p involved in SI-AKI was analyzed by bioinformatics method.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>The miR-625-5p expression was downregulated in sepsis as well as SI-AKI and has predictive value for sepsis as well as SI-AKI. In addition, miR-625-5p promoted LPS-induced cell proliferation and inhibited the levels of inflammatory cytokines.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>miR-625-5p may be a diagnostic biomarker for SI-AKI.</p>","PeriodicalId":14454,"journal":{"name":"International Urology and Nephrology","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Clinical significance of miR-625-5p in patients with sepsis-induced acute kidney injury based on bioinformatics analysis\",\"authors\":\"Baoying Gong, Jiayi Chen, Haizhen Yu, Shizhen Li\",\"doi\":\"10.1007/s11255-024-04209-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Purpose</h3><p>Sepsis often leads to a cluster of life-threatening symptoms affecting multiple organ systems. Among the organs most vulnerable to damage in this state is the kidney; those afflicted with severe sepsis frequently encounter acute kidney injury (AKI). The study was to investigate the diagnostic role of miR-625-5p in sepsis and sepsis-induced acute kidney injury (SI-AKI) and predict the possible pathways of miR-625-5p involved in SI-AKI by bioinformatics method.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>RT-qPCR was used to detect the level of miR-625-5p, and the diagnostic value of miR-625-5p was analyzed using ROC curve. The proliferation and the concentration of inflammatory factors of HK-2 cells induced by LPS were detected by CCK-8 and ELISA. The pathway of miR-625-5p involved in SI-AKI was analyzed by bioinformatics method.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>The miR-625-5p expression was downregulated in sepsis as well as SI-AKI and has predictive value for sepsis as well as SI-AKI. In addition, miR-625-5p promoted LPS-induced cell proliferation and inhibited the levels of inflammatory cytokines.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusion</h3><p>miR-625-5p may be a diagnostic biomarker for SI-AKI.</p>\",\"PeriodicalId\":14454,\"journal\":{\"name\":\"International Urology and Nephrology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Urology and Nephrology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s11255-024-04209-z\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"UROLOGY & NEPHROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Urology and Nephrology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11255-024-04209-z","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"UROLOGY & NEPHROLOGY","Score":null,"Total":0}
Clinical significance of miR-625-5p in patients with sepsis-induced acute kidney injury based on bioinformatics analysis
Purpose
Sepsis often leads to a cluster of life-threatening symptoms affecting multiple organ systems. Among the organs most vulnerable to damage in this state is the kidney; those afflicted with severe sepsis frequently encounter acute kidney injury (AKI). The study was to investigate the diagnostic role of miR-625-5p in sepsis and sepsis-induced acute kidney injury (SI-AKI) and predict the possible pathways of miR-625-5p involved in SI-AKI by bioinformatics method.
Methods
RT-qPCR was used to detect the level of miR-625-5p, and the diagnostic value of miR-625-5p was analyzed using ROC curve. The proliferation and the concentration of inflammatory factors of HK-2 cells induced by LPS were detected by CCK-8 and ELISA. The pathway of miR-625-5p involved in SI-AKI was analyzed by bioinformatics method.
Results
The miR-625-5p expression was downregulated in sepsis as well as SI-AKI and has predictive value for sepsis as well as SI-AKI. In addition, miR-625-5p promoted LPS-induced cell proliferation and inhibited the levels of inflammatory cytokines.
Conclusion
miR-625-5p may be a diagnostic biomarker for SI-AKI.
期刊介绍:
International Urology and Nephrology publishes original papers on a broad range of topics in urology, nephrology and andrology. The journal integrates papers originating from clinical practice.