对dsRNA诱导的RNA干扰和模式触发免疫的比较分析表明,对马铃薯病毒X的抗病毒反应效率不同

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Khouloud Necira, Lorenzo Contreras, Efstratios Kamargiakis, Mohamed Selim Kamoun, Tomás Canto, Francisco Tenllado
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引用次数: 0

摘要

双链 RNA(dsRNA)诱导的抗病毒反应包括 RNA 干扰(RNAi)和模式触发免疫(PTI),但它们对抗病毒防御的相对贡献尚不十分清楚。我们的目的是测试外源应用的 dsRNA 对烟曲霉中表达 GFP(PVX-GFP)的马铃薯病毒 X 的两层防御的影响。用序列特异性 dsRNA(RNAi)或非特异性 dsRNA(PTI)共同接种 PVX-GFP,结果表明,非特异性 dsRNA 可减少接种叶片和系统叶片中的病毒积累。然而,与能够触发 RNAi 反应的序列特异性 dsRNA 相比,非特异性 dsRNA 的抗病毒免疫诱导能力较差,而且植物容易受到系统性感染。对无法在细胞间移动的 PVX 突变体的研究表明,非特异性 dsRNA 对 PVX-GFP 的干扰是在单细胞水平上进行的。接下来,我们进行了RNA-seq分析,研究dsRNA单独或与携带dsRNA靶标序列或未携带dsRNA序列的病毒结合引发的转录组的异同。富集分析表明,钙、乙烯和 MAPK 信号转导等植物病原体信号通路的代表性过高,而这些通路是抗微生物 PTI 的典型通路。此外,与非靶标病毒相比,dsRNA 靶标病毒的转录组反应对防御的影响更大,这凸显了序列特异性 RNAi 与非特异性 PTI 反应之间的质的差异。总之,这些结果进一步加深了我们对植物抗病毒防御的理解,尤其是对非特异性 dsRNA 介导的 PTI 所作贡献的理解。我们设想,序列特异性 RNAi 和非特异性 PTI 途径都可能通过局部施用 dsRNA 触发,从而共同促进植物的抗病毒保护。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative analysis of RNA interference and pattern‐triggered immunity induced by dsRNA reveals different efficiencies in the antiviral response to potato virus X
Antiviral responses induced by double‐stranded RNA (dsRNA) include RNA interference (RNAi) and pattern‐triggered immunity (PTI), but their relative contributions to antiviral defence are not well understood. We aimed at testing the impact of exogenous applied dsRNA on both layers of defence against potato virus X expressing GFP (PVX‐GFP) in Nicotiana benthamiana. Co‐inoculation of PVX‐GFP with either sequence‐specific (RNAi) or nonspecific dsRNA (PTI) showed that nonspecific dsRNA reduced virus accumulation in both inoculated and systemic leaves. However, nonspecific dsRNA was a poor inducer of antiviral immunity compared to a sequence‐specific dsRNA capable of triggering the RNAi response, and plants became susceptible to systemic infection. Studies with a PVX mutant unable to move from cell to cell indicated that the interference with PVX‐GFP triggered by nonspecific dsRNA operated at the single‐cell level. Next, we performed RNA‐seq analysis to examine similarities and differences in the transcriptome triggered by dsRNA alone or in combination with viruses harbouring sequences targeted or not by dsRNA. Enrichment analysis showed an over‐representation of plant‐pathogen signalling pathways, such as calcium, ethylene and MAPK signalling, which are typical of antimicrobial PTI. Moreover, the transcriptomic response to the virus targeted by dsRNA had a greater impact on defence than the non‐targeted virus, highlighting qualitative differences between sequence‐specific RNAi and nonspecific PTI responses. Together, these results further our understanding of plant antiviral defence, particularly the contribution of nonspecific dsRNA‐mediated PTI. We envisage that both sequence‐specific RNAi and nonspecific PTI pathways may be triggered via topical application of dsRNA, contributing cumulatively to plant protection against viruses.
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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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