水解牛初乳提取物(BCFM)通过调节 MC1R-cAMP 信号通路抑制 B16F1 细胞中α-MSH 诱导的黑色素生成

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Jae Hyeok Choi, Taeil Kwak, Heejung Shin, Yang Hee Jo, Junil Kim, Younghwa Kim, Junoh Kim, Woo-Ram Lee
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引用次数: 0

摘要

牛初乳是牛出生后产生的第一种乳汁,是营养物质、免疫球蛋白、肽和生长因子的丰富天然来源。牛奶和乳清的生物转化改变了其固定性和生化特性。然而,水解牛初乳提取物(BCFM)在α-MSH 诱导的 B16F1 细胞中的细胞机制和抗黑色素合成作用尚未得到研究。在本研究中,我们通过检测 BCFM 对α-MSH 诱导的 B16F1 细胞的影响,研究了其抗黑色素生成的机制。在α-MSH存在或不存在的情况下,用BCFM处理细胞并共同培养24、48和72小时。此外,MC1R 和 cAMP 的表达水平,以及 CREB 和 PKA 的磷酸化水平都因α-MSH 的处理而增加。然而,同时用 BCFM 处理会导致这些因子和磷酸化的 MITF 显著下降。同时,B16F1 细胞中 TRP-1 和酪氨酸酶的表达量也有所下降。这些结果表明,BCFM 有可能通过调节α-MSH 诱导的 B16F1 细胞中的 MC1R-cAMP 信号通路来阻止黑色素生成过程。通过调节 B16F1 细胞中的 CREB/MITF 信号通路,施用 BCFM 可抑制 TRP-1 和/或酪氨酸酶的表达。我们认为水解牛初乳提取物(BCFM)适合用作皮肤美白或医药应用的新型活性剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Hydrolyzed cow colostrum extract (BCFM) inhibits alpha-MSH-induced melanogenesis in B16F1 cells via regulation of the MC1R-cAMP signaling pathway

Hydrolyzed cow colostrum extract (BCFM) inhibits alpha-MSH-induced melanogenesis in B16F1 cells via regulation of the MC1R-cAMP signaling pathway

Cow colostrum is the first milk produced after birth and is a rich natural source of nutrients, immunoglobulins, peptides, and growth factors. The bioconversion of milk and whey changes the immobilization and biochemical characterization. However, the cellular mechanism and the anti-melanin synthesis effects of hydrolyzed cow colostrum extract (BCFM) in alpha-MSH-induced B16F1 cells have not been examined. In this study, we investigated the anti-melanogenesis mechanism by examining the effects of BCFM in alpha-MSH-induced B16F1 cells. Cells were treated with BCFM in the presence or absence of alpha-MSH and co-cultured for 24, 48, and 72 h. The treatment of B16F1 cells with alpha-MSH resulted in the darkening of the color of the cells and induction of melanin synthesis. In addition, the expression levels of MC1R and cAMP, as well as phosphorylation levels of CREB and PKA, were increased by alpha-MSH treatment. However, concomitant treatment with BCFM resulted in a significant decrease in these factors and phosphorylated MITF. At the same time, the expressive amount of TRP-1 and tyrosinase was also decreased in B16F1 cells. These results demonstrate the potential of BCFM for the prevention of melanogenesis progression via the regulation of the MC1R-cAMP signaling pathway in alpha-MSH-induced B16F1 cells. The administration of BCFM suppressed the expression of TRP-1 and/or tyrosinase by regulating the CREB/MITF signaling pathways in the B16F1 cells. We propose that hydrolyzed cow colostrum extract (BCFM) is suitable for use as a novel active agent for skin whitening or pharmaceutical applications.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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