Ling Wu, Sihui Zhang, Mi Zhang, Yanjing Ou, Jiang Chen
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The role of target mRNA in OD-induced anxiety-like behaviours was evaluated and we preliminarily explored the possible downstream pathways.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>The results suggested that OD can induce and promote anxiety-like behaviours with/without chronic unpredictable mild stress. We found that Sirt1 was significantly downregulated within the hippocampus in OD mice. In addition, the downregulation of Sirt1 within the hippocampus in OD and control mice promoted anxiety-like behaviours, increased acetylated histone H3 expression and decreased Dnah12 transcription levels. 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引用次数: 0
摘要
背景以前的研究表明,咬合不协调(OD)可促进焦虑样行为。方法OD由雌性小鼠前交叉咬合产生。我们测量了各组小鼠的焦虑水平,并筛选了对照组和 OD 组小鼠的海马 mRNA 表达谱。结果表明,在有/无慢性不可预知轻度应激的情况下,OD可诱导和促进焦虑样行为。我们发现 Sirt1 在 OD 小鼠的海马中明显下调。此外,OD 小鼠和对照组小鼠海马中 Sirt1 的下调促进了焦虑样行为,增加了乙酰化组蛋白 H3 的表达,降低了 Dnah12 的转录水平。相反,在注射白藜芦醇的 OD 小鼠中,焦虑样行为得到缓解,海马中的 Sirt1 上调,同时乙酰化组蛋白 H3 表达减少,Dnah12 转录水平增加。在这一过程中,Sirt1 是调节 OD 诱导的焦虑样行为的有效因子。本研究探讨了 OD(一种应激源)对主要神经精神疾病病理生理学分子基因的影响。
Occlusal disharmony promotes anxiety-like behaviours by suppressing Sirt1
Background
Previous studies have indicated that occlusal disharmony (OD) can promote anxiety-like behaviours. However, the specific molecules involved in the development of anxiety-like behaviours and their underlying mechanisms remain unknown.
Methods
OD was produced by anterior crossbite of female mice. We measured the anxiety levels of mice in each group and screened the hippocampal mRNA expression profiles of mice in the control group and OD group. The role of target mRNA in OD-induced anxiety-like behaviours was evaluated and we preliminarily explored the possible downstream pathways.
Results
The results suggested that OD can induce and promote anxiety-like behaviours with/without chronic unpredictable mild stress. We found that Sirt1 was significantly downregulated within the hippocampus in OD mice. In addition, the downregulation of Sirt1 within the hippocampus in OD and control mice promoted anxiety-like behaviours, increased acetylated histone H3 expression and decreased Dnah12 transcription levels. In contrast, in OD mice subjected to an injection of resveratrol, there was a remission of anxiety-like behaviours and an upregulation of Sirt1 in the hippocampus, the effects of which were accompanied by decreased acetylated histone H3 expression and increased Dnah12 transcription levels.
Conclusions
OD leads to increased sensitivity to chronic stress in mice, resulting in anxiety-like behaviours. During this process, Sirt1 acts as an effective factor in the regulation of OD-induced anxiety-like behaviours.
Clinical relevance
OD, as a stressor, could induce anxiety-like behaviours. It investigates the impact of OD (a stressor) on the molecular genetic of the pathophysiology of major neuropsychiatric disorders.
期刊介绍:
The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.