检测榴莲糖中的葡萄球菌肠毒素 B (SEB) 的侧流免疫测定装置。

IF 1.8 4区 化学 Q3 CHEMISTRY, ANALYTICAL
Paquito E Relox,Katherine Ann C Israel,Yuki Hiruta,Daniel Citterio
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引用次数: 0

摘要

金黄色葡萄球菌肠毒素 B(SEB)是由金黄色葡萄球菌产生的一种强效肠毒素,与菲律宾的葡萄球菌食物中毒事件有关。使用横向流动免疫测定装置在采样点检测固体食品样本(如榴莲糖)中的这种毒素受到了样本净化(如离心)要求的限制。LFIA 设备在食品样品中的其他应用也存在这一问题。为了克服这一难题,本研究开发了一种横向流动免疫分析(LFIA)装置,能够检测未经纯化的榴莲糖样品中的 SEB。改进后的 LFIA 设备由三层玻璃纤维垫代替传统的纤维素纤维垫作为样品垫。与纤维素纤维垫不同,玻璃纤维样品垫起到了过滤的作用,允许 1:5 稀释的榴莲糖流动。将 LFIA 装置应用于加标 1:5 稀释的榴莲糖样品,SEB 的目测检测限为 5 纳克/毫升,比以前设计用于检测食品样品中 SEB 的 LFIA 装置低两倍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lateral flow immunoassay device to detect staphylococcal enterotoxin B (SEB) in durian candy.
Staphylococcal enterotoxin B (SEB), a potent enterotoxin produced by Staphylococcus aureus, has been implicated in incidences of Staphylococcal food poisoning in the Philippines. The use of lateral flow immunoassay devices to detect this toxin in solid food samples, like durian candy, at the point of sampling is constrained by the requirement for sample purification (e.g. centrifugation). This problem is also true with the other applications of LFIA devices on food samples. To overcome this challenge, a lateral flow immunoassay (LFIA) device capable of detecting SEB in unpurified durian candy sample was developed in this study. A modified LFIA device was assembled with three layers of glass fiber pads functioning as sample pads instead of a conventional cellulose fiber pad. Unlike with the cellulose fiber pad, the glass fiber sample pads acted as filter and allowed the flow of a 1:5 dilution of durian candy. The LFIA device applied to spiked 1:5 diluted durian candy samples achieved a visual limit of detection of 5 ng/mL for SEB, which is twofold lower than reported for previous LFIA devices designed to detect SEB in food samples.
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来源期刊
Analytical Sciences
Analytical Sciences 化学-分析化学
CiteScore
2.90
自引率
18.80%
发文量
232
审稿时长
1 months
期刊介绍: Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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