利用顶空气相色谱-串联质谱法对马血浆中的全氟化碳进行兴奋剂控制分析。

IF 2.6 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Drug Testing and Analysis Pub Date : 2024-09-18 DOI:10.1002/dta.3801

Fred K W Kong,April S Y Wong,Raymond K K Cheung,Terence S M Wan,Emmie N M Ho

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引用次数: 0

摘要

为了控制马匹运动中可能滥用全氟化碳作为氧气载体的情况，我们开发了一种简单灵敏的方法，以甲烷为试剂气体，利用负化学电离的气相色谱/串联质谱法检测马血浆中的全氟化碳，并进行了全面验证。该方法涵盖了血液替代产品中的七种活性成分--全氟化碳，具有良好的灵敏度和稳健性。该方法的检测限低至 0.01 纳克/毫升。据我们所知，这是首次报道用于筛查马生物样本中全氟碳化物的检测方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Doping Control Analysis of Perfluorocarbons in Equine Plasma by Headspace Gas Chromatography-Tandem Mass Spectrometry.

In order to control the potential misuse of perfluorocarbons as an oxygen carrier in equine sports, a simple and sensitive method for detecting perfluorocarbons in equine plasma by gas chromatography/tandem mass spectrometry using negative chemical ionization with methane as reagent gas has been developed and fully validated. The method covers seven perfluorocarbons, which are the active components in blood substitute products, and shows good sensitivity and robustness. Limits of detection as low as 0.01 ng/mL could be achieved by the method. To the best of our knowledge, this is the first report of a detection method for the screening of perfluorocarbons in equine biological samples.

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.