Ondrej Soucek, Marian Kacerovsky, Ivana Kacerovska Musilova, Jaroslav Stranik, Rudolf Kukla, Radka Bolehovska, Helena Hornychova, Ctirad Andrys
{"title":"与羊膜腔内炎症和胎儿炎症反应综合征有关的早产胎膜破裂妇女外周血和脐带血中不同白细胞亚群相对计数的变化","authors":"Ondrej Soucek, Marian Kacerovsky, Ivana Kacerovska Musilova, Jaroslav Stranik, Rudolf Kukla, Radka Bolehovska, Helena Hornychova, Ctirad Andrys","doi":"10.1111/aji.13926","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>The aim of this study was to evaluate changes in the relative counts of different leukocyte subsets in peripheral and umbilical cord blood in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to the presence of intraamniotic inflammation (IAI) and fetal inflammatory response syndrome (FIRS).</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Fifty-two women with singleton pregnancies complicated by PPROM were included in this study. From samples of peripheral and umbilical cord blood, relative counts of these leukocyte subpopulations were determined using multicolor flow cytometry: granulocytes, monocytes, lymphocytes, T cells and their subpopulations, B cells and their subpopulations, and NK cells and their subpopulations. IAI was defined as increased concentrations of interleukin 6 in the amniotic fluid. Amniotic fluid samples were obtained by transabdominal amniocentesis.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Women with IAI had higher relative counts of monocytes (<i>p</i> = 0.04) in peripheral blood. There was an increased relative number of granulocytes (<i>p</i> = 0.003) and a decreased number of lymphocytes (<i>p</i> = 0.0048), helper CD4+ T cells (<i>p</i> = 0.019), NK cells (<i>p</i> = 0.0001) within leukocytes, NK cells within lymphocytes (<i>p</i> = 0.003) and CD16+ NK cells within NK cells (<i>p</i> = 0.005) in umbilical cord blood samples of women with FIRS. However, after adjusting the results for gestational age at sampling, all differences disappeared.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The presence of IAI or FIRS is not accompanied by significant changes in the relative counts of immune cells in peripheral blood or umbilical cord blood in pregnancies complicated by PPROM.</p>\n </section>\n </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"92 3","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Changes in Relative Counts of Different Leukocyte Subpopulations in Peripheral and Umbilical Cord Blood of Women With Preterm Prelabor Rupture of Membranes With Respect to Intraamniotic Inflammation and Fetal Inflammatory Response Syndrome\",\"authors\":\"Ondrej Soucek, Marian Kacerovsky, Ivana Kacerovska Musilova, Jaroslav Stranik, Rudolf Kukla, Radka Bolehovska, Helena Hornychova, Ctirad Andrys\",\"doi\":\"10.1111/aji.13926\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>The aim of this study was to evaluate changes in the relative counts of different leukocyte subsets in peripheral and umbilical cord blood in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to the presence of intraamniotic inflammation (IAI) and fetal inflammatory response syndrome (FIRS).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Fifty-two women with singleton pregnancies complicated by PPROM were included in this study. From samples of peripheral and umbilical cord blood, relative counts of these leukocyte subpopulations were determined using multicolor flow cytometry: granulocytes, monocytes, lymphocytes, T cells and their subpopulations, B cells and their subpopulations, and NK cells and their subpopulations. IAI was defined as increased concentrations of interleukin 6 in the amniotic fluid. Amniotic fluid samples were obtained by transabdominal amniocentesis.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Women with IAI had higher relative counts of monocytes (<i>p</i> = 0.04) in peripheral blood. There was an increased relative number of granulocytes (<i>p</i> = 0.003) and a decreased number of lymphocytes (<i>p</i> = 0.0048), helper CD4+ T cells (<i>p</i> = 0.019), NK cells (<i>p</i> = 0.0001) within leukocytes, NK cells within lymphocytes (<i>p</i> = 0.003) and CD16+ NK cells within NK cells (<i>p</i> = 0.005) in umbilical cord blood samples of women with FIRS. 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Changes in Relative Counts of Different Leukocyte Subpopulations in Peripheral and Umbilical Cord Blood of Women With Preterm Prelabor Rupture of Membranes With Respect to Intraamniotic Inflammation and Fetal Inflammatory Response Syndrome
Objective
The aim of this study was to evaluate changes in the relative counts of different leukocyte subsets in peripheral and umbilical cord blood in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to the presence of intraamniotic inflammation (IAI) and fetal inflammatory response syndrome (FIRS).
Methods
Fifty-two women with singleton pregnancies complicated by PPROM were included in this study. From samples of peripheral and umbilical cord blood, relative counts of these leukocyte subpopulations were determined using multicolor flow cytometry: granulocytes, monocytes, lymphocytes, T cells and their subpopulations, B cells and their subpopulations, and NK cells and their subpopulations. IAI was defined as increased concentrations of interleukin 6 in the amniotic fluid. Amniotic fluid samples were obtained by transabdominal amniocentesis.
Results
Women with IAI had higher relative counts of monocytes (p = 0.04) in peripheral blood. There was an increased relative number of granulocytes (p = 0.003) and a decreased number of lymphocytes (p = 0.0048), helper CD4+ T cells (p = 0.019), NK cells (p = 0.0001) within leukocytes, NK cells within lymphocytes (p = 0.003) and CD16+ NK cells within NK cells (p = 0.005) in umbilical cord blood samples of women with FIRS. However, after adjusting the results for gestational age at sampling, all differences disappeared.
Conclusions
The presence of IAI or FIRS is not accompanied by significant changes in the relative counts of immune cells in peripheral blood or umbilical cord blood in pregnancies complicated by PPROM.
期刊介绍：
The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.
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