对来自 Odontobuthus doriae 蝎毒的两种新型多肽的蛋白质组分析。

IF 1.3 3区医学 Q3 CHEMISTRY, APPLIED

Journal of Asian Natural Products Research Pub Date : 2024-09-17 DOI:10.1080/10286020.2024.2403612

Jamil Zargan,Ehsan Jahangirian,Haider A Khan,Shakir Ali

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引用次数: 0

摘要

流行于东亚和伊朗的多瑞亚蝎毒尚未完全定性。本研究首次对多利亚蝎毒进行了蛋白质组学分析，以探索其作为药物的潜力。2D-PAGE 分析显示有 96 个蛋白质点，等电点在 3 到 9 之间，分子量在 6.6 到 205 kDa 之间。通过 HPLC 分离出 14 个毒素馏分，SDS-PAGE 显示出 7 条蛋白质带，分子量在 3.8 至 182 kDa 之间。MALDI-TOF MS 鉴定出了肽 1 和肽 2，它们与血红蛋白 beta-2 链和伴侣素 HSP60 相似，并提出了 P1 和 P2 的潜在治疗用途。

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Proteomic analysis of two novel peptides from the Odontobuthus doriae scorpion venom.

The venom of the Odontobuthus doriae scorpion, prevalent in East Asia and Iran, has not been fully characterized. This study provides the first proteomic profile of O. doriae venom to explore its potential as a medical. 2D-PAGE analysis revealed 96 protein spots with isoelectric points from 3 to 9 and molecular weights between 6.6 to 205 kDa. Fourteen toxin fractions were isolated via HPLC, and SDS-PAGE showed seven protein bands ranging from 3.8 to 182 kDa. MALDI-TOF MS identified Peptide 1 and Peptide 2, resembling Hemoglobin beta-2 chain and Chaperonin HSP60 and suggest potential therapeutic applications for P1 and P2.

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来源期刊

Journal of Asian Natural Products Research 医学-药学

CiteScore

3.20

自引率

5.90%

发文量

审稿时长

2.3 months

期刊介绍： The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures. All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.