BIRC5-206 的减少通过海绵状 miR-145-5p 促进鼻咽癌的上皮-间质转化

IF 1.7 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Weihua Xu, Junjie Hu, Zhichao Ma, Wanyi Feng, Wei Gong, Shengmiao Fu, Xinping Chen
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引用次数: 0

摘要

转移是导致晚期鼻咽癌(NPC)预后不良的重要原因。我们之前的研究表明,鼻咽癌中 BIRC5-206 的表达减少会促进疾病进展。然而,BIRC5-206 在鼻咽癌的侵袭和转移中的作用尚未完全阐明。在本研究中,我们的目的是探索 BIRC5-206 在鼻咽癌中的生物学功能和潜在机制。此外,我们还利用 C666 细胞建立了肺侵袭性鼻咽癌小鼠模型,以评估 BIRC5-206 对鼻咽癌转移的影响。我们的研究结果表明,沉默 BIRC5-206 会抑制鼻咽癌细胞的凋亡并增强其侵袭性,而过表达 BIRC5-206 则会逆转这些影响。此外,无论是在体内还是体外,BIRC5-206表达量减少都会显著增加N-粘连蛋白和Vimentin的表达,同时降低E-粘连蛋白和occludin的水平。此外,沉默 BIRC5-206 能明显增加肺组织中侵袭性病灶的形成。拯救实验进一步证实,BIRC5-206表达的减少通过调节miR-145-5p/CD40信号通路促进了鼻咽癌的转移。总之,我们的研究表明,BIRC5-206 可作为诊断和治疗鼻咽癌的潜在预后生物标志物和治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Decreased BIRC5-206 promotes epithelial–mesenchymal transition in nasopharyngeal carcinoma through sponging miR-145-5p
Metastasis significantly contributes to the poor prognosis of advanced nasopharyngeal carcinoma (NPC). Our prior studies have demonstrated a decrease in BIRC5-206 expression in NPC, which promotes disease progression. However, the role of BIRC5-206 in the invasion and metastasis of NPC has not been fully elucidated. In this study, our objective was to explore the biological function and underlying mechanisms of BIRC5-206 in NPC. Additionally, we established an NPC mouse model of lung invasiveness using C666 cells to assess the impact of BIRC5-206 on NPC metastasis. Our results revealed that silencing BIRC5-206 inhibited apoptosis and enhanced the invasion of NPC cells, whereas its overexpression reversed these effects. Moreover, decreased BIRC5-206 expression significantly increased N-cadherin and Vimentin expression while reducing E-cadherin and occludin levels, both in vivo and in vitro. Additionally, silencing BIRC5-206 markedly augmented the formation of invasive foci in lung tissues. Rescue experiments further confirmed that decreased BIRC5-206 expression facilitates NPC metastasis via modulation of the miR-145-5p/CD40 signaling pathway. In summary, our study suggests that BIRC5-206 may serve as a potential prognostic biomarker and therapeutic target in the diagnosis and treatment of NPC.
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来源期刊
Open Medicine
Open Medicine Medicine-General Medicine
CiteScore
3.00
自引率
0.00%
发文量
153
审稿时长
20 weeks
期刊介绍: Open Medicine is an open access journal that provides users with free, instant, and continued access to all content worldwide. The primary goal of the journal has always been a focus on maintaining the high quality of its published content. Its mission is to facilitate the exchange of ideas between medical science researchers from different countries. Papers connected to all fields of medicine and public health are welcomed. Open Medicine accepts submissions of research articles, reviews, case reports, letters to editor and book reviews.
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