{"title":"金属硫蛋白基因 OsMT2b 在水稻干旱和镉离子胁迫中的参与","authors":"Yanxin Chen, Ying He, Yibin Pan, Yunyi Wen, Lili Zhu, Jieer Gao, Weiting Chen, Dagang Jiang","doi":"10.1186/s12284-024-00740-w","DOIUrl":null,"url":null,"abstract":"<p>Abiotic stress is one of the major factors restricting the production of rice (<i>Oryza sativa</i> L.). Developing rice varieties with dual abiotic stress tolerance is essential to ensure sustained rice production, which is necessary to illustrate the regulation mechanisms underlying dual stress tolerance. At present, only a few genes that regulate dual abiotic stress tolerance have been reported. In this study, we determined that the expression of <i>OsMT2b</i> was induced by both drought and Cd<sup>2+</sup> stress. After stress treatment, <i>OsMT2b</i>-overexpression lines exhibited enhanced drought tolerance and better physiological performance in terms of relative water content and electrolyte leakage compared with wild-type (WT). Further analysis indicated that ROS levels were lower in <i>OsMT2b</i>-overexpression lines than in WT following stress treatment, suggesting that <i>OsMT2b</i>-overexpression lines had a stronger ability to scavenge ROS under stress. Reverse transcription-quantitative PCR (RT-qPCR) results demonstrated that under drought stress, <i>OsMT2b</i> influenced the expression of genes involved in ROS scavenging to enhance drought tolerance in rice. In addition, <i>OsMT2b</i>-overexpression plants displayed increased tolerance to Cd<sup>2+</sup> stress, and physiological assessment results were consistent with the observed phenotypic improvements. Thus, enhancing ROS scavenging ability through <i>OsMT2b</i> overexpression is a novel strategy to boost rice tolerance to both drought and Cd<sup>2+</sup> stress, offering a promising approach for developing rice germplasm with enhanced resistance to the abiotic stressors.</p>","PeriodicalId":21408,"journal":{"name":"Rice","volume":"4 1","pages":""},"PeriodicalIF":4.8000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Involvement of the Metallothionein gene OsMT2b in Drought and Cadmium Ions Stress in Rice\",\"authors\":\"Yanxin Chen, Ying He, Yibin Pan, Yunyi Wen, Lili Zhu, Jieer Gao, Weiting Chen, Dagang Jiang\",\"doi\":\"10.1186/s12284-024-00740-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Abiotic stress is one of the major factors restricting the production of rice (<i>Oryza sativa</i> L.). Developing rice varieties with dual abiotic stress tolerance is essential to ensure sustained rice production, which is necessary to illustrate the regulation mechanisms underlying dual stress tolerance. At present, only a few genes that regulate dual abiotic stress tolerance have been reported. In this study, we determined that the expression of <i>OsMT2b</i> was induced by both drought and Cd<sup>2+</sup> stress. After stress treatment, <i>OsMT2b</i>-overexpression lines exhibited enhanced drought tolerance and better physiological performance in terms of relative water content and electrolyte leakage compared with wild-type (WT). Further analysis indicated that ROS levels were lower in <i>OsMT2b</i>-overexpression lines than in WT following stress treatment, suggesting that <i>OsMT2b</i>-overexpression lines had a stronger ability to scavenge ROS under stress. Reverse transcription-quantitative PCR (RT-qPCR) results demonstrated that under drought stress, <i>OsMT2b</i> influenced the expression of genes involved in ROS scavenging to enhance drought tolerance in rice. In addition, <i>OsMT2b</i>-overexpression plants displayed increased tolerance to Cd<sup>2+</sup> stress, and physiological assessment results were consistent with the observed phenotypic improvements. Thus, enhancing ROS scavenging ability through <i>OsMT2b</i> overexpression is a novel strategy to boost rice tolerance to both drought and Cd<sup>2+</sup> stress, offering a promising approach for developing rice germplasm with enhanced resistance to the abiotic stressors.</p>\",\"PeriodicalId\":21408,\"journal\":{\"name\":\"Rice\",\"volume\":\"4 1\",\"pages\":\"\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rice\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1186/s12284-024-00740-w\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rice","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s12284-024-00740-w","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Involvement of the Metallothionein gene OsMT2b in Drought and Cadmium Ions Stress in Rice
Abiotic stress is one of the major factors restricting the production of rice (Oryza sativa L.). Developing rice varieties with dual abiotic stress tolerance is essential to ensure sustained rice production, which is necessary to illustrate the regulation mechanisms underlying dual stress tolerance. At present, only a few genes that regulate dual abiotic stress tolerance have been reported. In this study, we determined that the expression of OsMT2b was induced by both drought and Cd2+ stress. After stress treatment, OsMT2b-overexpression lines exhibited enhanced drought tolerance and better physiological performance in terms of relative water content and electrolyte leakage compared with wild-type (WT). Further analysis indicated that ROS levels were lower in OsMT2b-overexpression lines than in WT following stress treatment, suggesting that OsMT2b-overexpression lines had a stronger ability to scavenge ROS under stress. Reverse transcription-quantitative PCR (RT-qPCR) results demonstrated that under drought stress, OsMT2b influenced the expression of genes involved in ROS scavenging to enhance drought tolerance in rice. In addition, OsMT2b-overexpression plants displayed increased tolerance to Cd2+ stress, and physiological assessment results were consistent with the observed phenotypic improvements. Thus, enhancing ROS scavenging ability through OsMT2b overexpression is a novel strategy to boost rice tolerance to both drought and Cd2+ stress, offering a promising approach for developing rice germplasm with enhanced resistance to the abiotic stressors.
期刊介绍:
Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.