金属硫蛋白基因 OsMT2b 在水稻干旱和镉离子胁迫中的参与

IF 4.8 1区 农林科学 Q1 AGRONOMY
Rice Pub Date : 2024-09-19 DOI:10.1186/s12284-024-00740-w
Yanxin Chen, Ying He, Yibin Pan, Yunyi Wen, Lili Zhu, Jieer Gao, Weiting Chen, Dagang Jiang
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引用次数: 0

摘要

非生物胁迫是制约水稻生产的主要因素之一。培育具有双重抗逆性的水稻品种对于确保水稻的持续生产至关重要,这就需要说明双重抗逆性的调控机制。目前,只有少数几个调控双重抗逆性的基因被报道。在本研究中,我们发现干旱和 Cd2+ 胁迫都会诱导 OsMT2b 的表达。与野生型(WT)相比,OsMT2b过表达株在胁迫处理后表现出更强的耐旱性,在相对含水量和电解质渗漏方面有更好的生理表现。进一步的分析表明,在胁迫处理后,OsMT2b表达株的ROS水平低于WT,这表明OsMT2b表达株在胁迫下清除ROS的能力更强。反转录定量 PCR(RT-qPCR)结果表明,在干旱胁迫下,OsMT2b 影响了参与清除 ROS 的基因的表达,从而增强了水稻的抗旱能力。此外,OsMT2b高表达植株对Cd2+胁迫的耐受性增强,生理评估结果与观察到的表型改善一致。因此,通过过表达 OsMT2b 来增强清除 ROS 的能力是提高水稻对干旱和 Cd2+ 胁迫耐受性的一种新策略,为开发具有更强抗非生物胁迫能力的水稻种质提供了一种可行的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Involvement of the Metallothionein gene OsMT2b in Drought and Cadmium Ions Stress in Rice

Involvement of the Metallothionein gene OsMT2b in Drought and Cadmium Ions Stress in Rice

Abiotic stress is one of the major factors restricting the production of rice (Oryza sativa L.). Developing rice varieties with dual abiotic stress tolerance is essential to ensure sustained rice production, which is necessary to illustrate the regulation mechanisms underlying dual stress tolerance. At present, only a few genes that regulate dual abiotic stress tolerance have been reported. In this study, we determined that the expression of OsMT2b was induced by both drought and Cd2+ stress. After stress treatment, OsMT2b-overexpression lines exhibited enhanced drought tolerance and better physiological performance in terms of relative water content and electrolyte leakage compared with wild-type (WT). Further analysis indicated that ROS levels were lower in OsMT2b-overexpression lines than in WT following stress treatment, suggesting that OsMT2b-overexpression lines had a stronger ability to scavenge ROS under stress. Reverse transcription-quantitative PCR (RT-qPCR) results demonstrated that under drought stress, OsMT2b influenced the expression of genes involved in ROS scavenging to enhance drought tolerance in rice. In addition, OsMT2b-overexpression plants displayed increased tolerance to Cd2+ stress, and physiological assessment results were consistent with the observed phenotypic improvements. Thus, enhancing ROS scavenging ability through OsMT2b overexpression is a novel strategy to boost rice tolerance to both drought and Cd2+ stress, offering a promising approach for developing rice germplasm with enhanced resistance to the abiotic stressors.

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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
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