Atanu Maji, Debarpan Mitra, Amitav Biswas, Moumita Ghosh, Rahul Naskar, Saswati Gharami Nabendu Murmu and Tapan K. Mondal
{"title":"调节 DNA 交互探针的结合位点:高效的 Al3+ 色荧光精细识别及其活细胞生物成像技术","authors":"Atanu Maji, Debarpan Mitra, Amitav Biswas, Moumita Ghosh, Rahul Naskar, Saswati Gharami Nabendu Murmu and Tapan K. Mondal","doi":"10.1039/D4SD00242C","DOIUrl":null,"url":null,"abstract":"<p >Herein, a chromone-based simple reversible fluorescent “turn-on” probe, HMCP [6-(hydroxymethyl)-<em>N</em>′-((6-methyl-4-oxo-4<em>H</em>-chromen-3-yl)methylene)picolinohydrazide], was successfully utilized to detect Al<small><sup>3+</sup></small> over a group of other coexisting metal cations in MeOH/H<small><sub>2</sub></small>O (9 : 1, v/v) (HEPES buffer, pH = 7.2). The “turn on” emission response along with the effective enhancement of the fluorescence intensity upon addition of Al<small><sup>3+</sup></small> can be attributed to the inhibition of photo-induced electron transfer (PET) and C<img>N isomerization, as well as the initiation of chelation-enhanced-fluorescence (CHEF). The HMCP sensor binds Al<small><sup>3+</sup></small> in a 1 : 1 stoichiometry with an excellent binding constant and good detection limit on the orders of 10<small><sup>3</sup></small> M<small><sup>−1</sup></small> and 10<small><sup>−7</sup></small> M, respectively. The mode of binding interaction between HMCP with Al<small><sup>3+</sup></small> was evidenced by <small><sup>1</sup></small>H NMR titration, HRMS, and Job's plot analyses. Theoretical calculations and molecular logic gate applications were also used to demonstrate the binding mode. A DNA binding study was also executed to elucidate the possible bioactivity of the probe and found that HMCP interacts with DNA more effectively than the other analogues studied. Furthermore, the applicability of the probe in a live cell imaging study indicated that HMCP is highly efficient for the detection of exogenous Al<small><sup>3+</sup></small> in living cells. In addition, real water sample analysis and a dip-stick experiment demonstrate that the probe can be used in a wide range of practical and convenient applications.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00242c?page=search","citationCount":"0","resultStr":"{\"title\":\"Modulation of the binding sites for an adaptable DNA interactive probe: efficient chromo-fluorogenic recognition of Al3+ and live cell bioimaging†\",\"authors\":\"Atanu Maji, Debarpan Mitra, Amitav Biswas, Moumita Ghosh, Rahul Naskar, Saswati Gharami Nabendu Murmu and Tapan K. Mondal\",\"doi\":\"10.1039/D4SD00242C\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Herein, a chromone-based simple reversible fluorescent “turn-on” probe, HMCP [6-(hydroxymethyl)-<em>N</em>′-((6-methyl-4-oxo-4<em>H</em>-chromen-3-yl)methylene)picolinohydrazide], was successfully utilized to detect Al<small><sup>3+</sup></small> over a group of other coexisting metal cations in MeOH/H<small><sub>2</sub></small>O (9 : 1, v/v) (HEPES buffer, pH = 7.2). The “turn on” emission response along with the effective enhancement of the fluorescence intensity upon addition of Al<small><sup>3+</sup></small> can be attributed to the inhibition of photo-induced electron transfer (PET) and C<img>N isomerization, as well as the initiation of chelation-enhanced-fluorescence (CHEF). The HMCP sensor binds Al<small><sup>3+</sup></small> in a 1 : 1 stoichiometry with an excellent binding constant and good detection limit on the orders of 10<small><sup>3</sup></small> M<small><sup>−1</sup></small> and 10<small><sup>−7</sup></small> M, respectively. The mode of binding interaction between HMCP with Al<small><sup>3+</sup></small> was evidenced by <small><sup>1</sup></small>H NMR titration, HRMS, and Job's plot analyses. Theoretical calculations and molecular logic gate applications were also used to demonstrate the binding mode. A DNA binding study was also executed to elucidate the possible bioactivity of the probe and found that HMCP interacts with DNA more effectively than the other analogues studied. Furthermore, the applicability of the probe in a live cell imaging study indicated that HMCP is highly efficient for the detection of exogenous Al<small><sup>3+</sup></small> in living cells. In addition, real water sample analysis and a dip-stick experiment demonstrate that the probe can be used in a wide range of practical and convenient applications.</p>\",\"PeriodicalId\":74786,\"journal\":{\"name\":\"Sensors & diagnostics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00242c?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sensors & diagnostics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/sd/d4sd00242c\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors & diagnostics","FirstCategoryId":"1085","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/sd/d4sd00242c","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Modulation of the binding sites for an adaptable DNA interactive probe: efficient chromo-fluorogenic recognition of Al3+ and live cell bioimaging†
Herein, a chromone-based simple reversible fluorescent “turn-on” probe, HMCP [6-(hydroxymethyl)-N′-((6-methyl-4-oxo-4H-chromen-3-yl)methylene)picolinohydrazide], was successfully utilized to detect Al3+ over a group of other coexisting metal cations in MeOH/H2O (9 : 1, v/v) (HEPES buffer, pH = 7.2). The “turn on” emission response along with the effective enhancement of the fluorescence intensity upon addition of Al3+ can be attributed to the inhibition of photo-induced electron transfer (PET) and CN isomerization, as well as the initiation of chelation-enhanced-fluorescence (CHEF). The HMCP sensor binds Al3+ in a 1 : 1 stoichiometry with an excellent binding constant and good detection limit on the orders of 103 M−1 and 10−7 M, respectively. The mode of binding interaction between HMCP with Al3+ was evidenced by 1H NMR titration, HRMS, and Job's plot analyses. Theoretical calculations and molecular logic gate applications were also used to demonstrate the binding mode. A DNA binding study was also executed to elucidate the possible bioactivity of the probe and found that HMCP interacts with DNA more effectively than the other analogues studied. Furthermore, the applicability of the probe in a live cell imaging study indicated that HMCP is highly efficient for the detection of exogenous Al3+ in living cells. In addition, real water sample analysis and a dip-stick experiment demonstrate that the probe can be used in a wide range of practical and convenient applications.