调节 DNA 交互探针的结合位点:高效的 Al3+ 色荧光精细识别及其活细胞生物成像技术

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Atanu Maji, Debarpan Mitra, Amitav Biswas, Moumita Ghosh, Rahul Naskar, Saswati Gharami Nabendu Murmu and Tapan K. Mondal
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引用次数: 0

摘要

本文成功引入了一种基于铬酮的简单可逆荧光 "开启 "探针 HMCP [6-(Hydroxymethyl)-N'-((6-methyl-4-oxo-4H-chromen-3-yl)methylene)picolinohydrazide] ,用于在 pH 值为 7.2 的甲醇中检测 Al3+,而不是其他共存金属离子。加入 Al3+ 后的 "开启 "发射响应和荧光强度的有效增强可归因于光诱导电子转移(PET)和 C=N 异构化的抑制以及螯合增强荧光(CHEF)的启动。传感器 HMCP 与 Al3+ 的结合比例为 1:1,其结合常数和检测限分别为 103 M-1 和 10-7 M。通过 1H-NMR 滴定、HRMS、约伯图分析、理论计算以及分子逻辑门应用,证明了 HMCP 与 Al3+ 的结合模式。为了阐明可能的生物活性,还进行了 DNA 结合研究,结果发现 HMCP 与 DNA 的相互作用比其他类似物更有效。此外,活细胞成像研究表明,HMCP 能高效检测活细胞中的外源 Al3+。此外,真实样品分析和浸渍棒实验也为探针的实际应用提供了广泛的便利。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Modulation of the binding sites for an adaptable DNA interactive probe: efficient chromo-fluorogenic recognition of Al3+ and live cell bioimaging†

Modulation of the binding sites for an adaptable DNA interactive probe: efficient chromo-fluorogenic recognition of Al3+ and live cell bioimaging†

Herein, a chromone-based simple reversible fluorescent “turn-on” probe, HMCP [6-(hydroxymethyl)-N′-((6-methyl-4-oxo-4H-chromen-3-yl)methylene)picolinohydrazide], was successfully utilized to detect Al3+ over a group of other coexisting metal cations in MeOH/H2O (9 : 1, v/v) (HEPES buffer, pH = 7.2). The “turn on” emission response along with the effective enhancement of the fluorescence intensity upon addition of Al3+ can be attributed to the inhibition of photo-induced electron transfer (PET) and CN isomerization, as well as the initiation of chelation-enhanced-fluorescence (CHEF). The HMCP sensor binds Al3+ in a 1 : 1 stoichiometry with an excellent binding constant and good detection limit on the orders of 103 M−1 and 10−7 M, respectively. The mode of binding interaction between HMCP with Al3+ was evidenced by 1H NMR titration, HRMS, and Job's plot analyses. Theoretical calculations and molecular logic gate applications were also used to demonstrate the binding mode. A DNA binding study was also executed to elucidate the possible bioactivity of the probe and found that HMCP interacts with DNA more effectively than the other analogues studied. Furthermore, the applicability of the probe in a live cell imaging study indicated that HMCP is highly efficient for the detection of exogenous Al3+ in living cells. In addition, real water sample analysis and a dip-stick experiment demonstrate that the probe can be used in a wide range of practical and convenient applications.

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