Jean Cappello, Jonas Miguet, Adrien Dewandre, Lucie Ergot, Sylvain Gabriele, Jean Septavaux and Benoit Scheid
{"title":"控制水基藻酸盐微珠的尺寸和弹性模量","authors":"Jean Cappello, Jonas Miguet, Adrien Dewandre, Lucie Ergot, Sylvain Gabriele, Jean Septavaux and Benoit Scheid","doi":"10.1039/D4SM00260A","DOIUrl":null,"url":null,"abstract":"<p >The fabrication of microgels, particularly those ranging from tens to hundreds of micrometers in size, represents a thriving area of research, particularly for biologists seeking controlled and isotropic media for cell encapsulation. In this article, we present a novel and robust method for producing structurally homogeneous alginate beads with a reduced environmental footprint, employing a co-flow focusing microfluidic device. These beads can be easily recovered in an oil-free aqueous medium, making the fabrication method highly suitable for diverse applications. We demonstrate precise control over the production of perfectly spherical beads across a wide range of diameters, from about 30 to 300 μm. We then measure Young's moduli of the beads, revealing a wide accessible range from 90 Pa to 11 kPa, contingent upon controlling the type (<em>e.g.</em> chain length) and concentration of alginate.</p>","PeriodicalId":103,"journal":{"name":"Soft Matter","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Controlling the size and elastic modulus of in-aqueous alginate micro-beads\",\"authors\":\"Jean Cappello, Jonas Miguet, Adrien Dewandre, Lucie Ergot, Sylvain Gabriele, Jean Septavaux and Benoit Scheid\",\"doi\":\"10.1039/D4SM00260A\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >The fabrication of microgels, particularly those ranging from tens to hundreds of micrometers in size, represents a thriving area of research, particularly for biologists seeking controlled and isotropic media for cell encapsulation. In this article, we present a novel and robust method for producing structurally homogeneous alginate beads with a reduced environmental footprint, employing a co-flow focusing microfluidic device. These beads can be easily recovered in an oil-free aqueous medium, making the fabrication method highly suitable for diverse applications. We demonstrate precise control over the production of perfectly spherical beads across a wide range of diameters, from about 30 to 300 μm. We then measure Young's moduli of the beads, revealing a wide accessible range from 90 Pa to 11 kPa, contingent upon controlling the type (<em>e.g.</em> chain length) and concentration of alginate.</p>\",\"PeriodicalId\":103,\"journal\":{\"name\":\"Soft Matter\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Soft Matter\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/sm/d4sm00260a\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Soft Matter","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/sm/d4sm00260a","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Controlling the size and elastic modulus of in-aqueous alginate micro-beads
The fabrication of microgels, particularly those ranging from tens to hundreds of micrometers in size, represents a thriving area of research, particularly for biologists seeking controlled and isotropic media for cell encapsulation. In this article, we present a novel and robust method for producing structurally homogeneous alginate beads with a reduced environmental footprint, employing a co-flow focusing microfluidic device. These beads can be easily recovered in an oil-free aqueous medium, making the fabrication method highly suitable for diverse applications. We demonstrate precise control over the production of perfectly spherical beads across a wide range of diameters, from about 30 to 300 μm. We then measure Young's moduli of the beads, revealing a wide accessible range from 90 Pa to 11 kPa, contingent upon controlling the type (e.g. chain length) and concentration of alginate.