利用金属光栅的相位表面等离子体共振技术实现对 SARS-CoV-2 病毒核酸的快速、灵敏和多重检测。

IF 2.9 2区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Biomedical optics express Pub Date : 2024-08-13 DOI:10.1364/boe.535051

Shuai Sun,Yuanyuan Zhao,Yuxuan Hao,Pan Xue,Xiaoyang Guo,Wei Zhang,Chunxiang Zhang,Qiongdi Zhang,Wenhua Zhou,Hock Chun Ong,Xuefeng Yu,Zeren Li,Jia Li,Jianquan Yao

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引用次数: 0

摘要

本文提出了一种基于金属光栅相位质子光谱学的快速、灵敏和易于实施的病原体核酸检测方法。利用干涉光刻技术制作了质子传感器，并用单链 DNA 探针对特定的 SARS-CoV-2 目标进行了功能化。该生物传感器可在 5 分钟内检测到 40 fM 的病毒核酸；此外，结合重组酶聚合酶扩增，其检测能力可达 1 aM（0.6 拷贝/微升）。此外，多路复用传感系统还证明可在单个传感芯片上同时检测三种基因组序列，从而提高了诊断准确性并实现了高通量检测。

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Rapid, sensitive and multiplexed detection of SARS-CoV-2 viral nucleic acids enabled by phase-based surface plasmon resonance of metallic gratings.

A rapid, sensitive and easy-to-implement approach is proposed for the detection of pathogenic nucleic acids based on phase-based plasmonic spectroscopy of metallic gratings. The plasmonic sensors were fabricated using interference lithography and functionalized with single-stranded DNA probes to specific target SARS-CoV-2. The biosensor achieved the detection of 40 fM viral nucleic acids within 5 min; furthermore, a detection capability of 1 aM (0.6 copies/µL) was acquired when combining with the recombinase polymerase amplification. Additionally, the multiplexed sensing system was demonstrated to simultaneously detect three genomic sequences on a single sensor chip, thereby enhancing diagnostic accuracy and enabling high-throughput detection.

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来源期刊

Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS

CiteScore

6.80

自引率

11.80%

发文量

633

审稿时长

1 months

期刊介绍： The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.