利用 Neocosmospora Solani 内生真菌提取物生物生产纳米银颗粒:其体外抗菌和抗癌特性

IF 3.9 3区 化学 Q2 POLYMER SCIENCE
Kistu Singh Nongthombam, Surendirakumar Kannaiah, Prabhu Raju, Lakshmanan Govindan, Shyamkesho Singh Mutum, Radha Raman Pandey
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引用次数: 0

摘要

纳米材料的生物合成具有很大的优势,生物相容性好,可生产有价值的材料。在本研究中,我们展示了一种可持续且生物兼容的方法,即利用内生真菌 Neocosmospora solani(NS)从 Anaphalis contorta 茎中提取的提取物合成银纳米粒子(Ag NPs)。体外研究评估了生物活性 NS 介导的 AgNPs 的抗癌和抗菌潜力。通过紫外可见光、XRD、傅立叶变换红外光谱、FE-SEM、DLS 和 Zeta 电位分析仪对合成的 NS-AgNPs 的理化特性进行了系统研究。具体而言,紫外可见光谱证实了生物合成的 NS-AgNPs 在 432 纳米波长处的存在,而 XRD 分析则证实了其晶体性质。傅立叶变换红外光谱证实了生物大分子中存在作为纳米粒子封端剂的官能团。扫描电镜用于评估 AgNPs 的形状。动态光散射(DLS)表明,平均粒径为 362.3 nm,zeta 电位为 - 0.168 mV,且只有一个峰值。生物系统技术可在合成后 2 个月内产生稳定的 AgNPs。此外,NS-AgNPs 对人类致病细菌菌株具有极佳的抗菌效果。NS-AgNPs对A549肺癌细胞的抗癌评估结果显示,在AO/EB和Hoechst染色试验中,NS-AgNPs都出现了剂量依赖性细胞毒性和形态学变化。未来,它可能会成为一种用于生物医学应用的优良抗生素和抗癌材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biogenic Production of Silver Nanoparticles Using Neocosmospora Solani Endophytic Fungal Extract: Their In Vitro Antibacterial and Anticancer Properties

Biogenic Production of Silver Nanoparticles Using Neocosmospora Solani Endophytic Fungal Extract: Their In Vitro Antibacterial and Anticancer Properties

The biogenic synthesis of nanomaterials has great advantages, biocompatible, and valuable material production. In this study, we demonstrated a sustainable and biocompatible approach for the synthesis of Ag nanoparticles (Ag NPs) using the endophytic fungi Neocosmospora solani (NS) extract isolated from the Anaphalis contorta stem. The anticancer and antibacterial potential of bioactive NS mediated AgNPs was evaluated by in vitro studies. The physicochemical characteristics of synthesized NS-AgNPs were systematically investigated by UV-vis, XRD, FTIR, FE-SEM, DLS and Zeta potential analyzer. In details, UV-visible spectroscopy confirmed the presence of biosynthesized NS-AgNPs at 432 nm, while XRD analysis confirmed their crystal nature. The FTIR spectrum confirmed the presence of functional groups in biomolecules that act as a capping agent for the nanoparticles. SEM was used to evaluate the shape of AgNPs. Dynamic light scattering (DLS) indicated that the average particle size is 362.3 nm, a zeta potential was − 0.168 mV with a single peak. The biosystem technique produced stable AgNPs up to 2 months following synthesis. In addition, the NS-AgNPs were exposed to excellent antibacterial efficacy against human pathogenic bacterial strains. The results of the anticancer assessment of NS-AgNPs against A549 lung cancer cells revealed that the dose-dependent cytotoxic and morphological changes have been reported in both AO/EB and Hoechst’s staining assays. In the future, it might be an excellent antibiotic and anticancer material for biomedical applications.

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来源期刊
CiteScore
8.30
自引率
7.50%
发文量
335
审稿时长
1.8 months
期刊介绍: Journal of Inorganic and Organometallic Polymers and Materials [JIOP or JIOPM] is a comprehensive resource for reports on the latest theoretical and experimental research. This bimonthly journal encompasses a broad range of synthetic and natural substances which contain main group, transition, and inner transition elements. The publication includes fully peer-reviewed original papers and shorter communications, as well as topical review papers that address the synthesis, characterization, evaluation, and phenomena of inorganic and organometallic polymers, materials, and supramolecular systems.
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